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The map label for this gene is nrdE

Identifier: 15672957

GI number: 15672957

Start: 1002635

End: 1004803

Strand: Reverse

Name: nrdE

Synonym: L0294

Alternate gene names: 15672957

Gene position: 1004803-1002635 (Counterclockwise)

Preceding gene: 15672958

Following gene: 15672956

Centisome position: 42.48

GC content: 35.45

Gene sequence:

>2169_bases
ATGTCTCTAAAACAACTTAAAGATGTAACTTATTTCAAGCTTAATAACGAGATTAATATTCCCGTTAATGGTAGCATTCC
TCTTGCTAAAGATAAAGAAGCAATTACTGCTTTTTTTAAAGAAAATGTCGAACCTAATCGTTATGAAGCCGATACATATA
TCGATAAACTCAATTGGCTCGTTAAAGAAGAATATCTAGAAGCTGAGTTTCTTGAAAAATATAAACCCGAATTTATTACC
AATCTTCGGAAAGAATTGAGTAAATTTGAATTTCATTTTGATTCATTTATGGCTGCTTACAAATTTTATAATCAATATGC
CATGAAAAACAATGACGGTTCTCTCTATCTTGAAGATTTTGAAGACCGTGTGTTAATGAATGCTTTATACATGGCTGACG
GCGATGAAAAATTAGCTAATAATCTTGCTTTTGCAATGATTAATCGTCGTTATCAACCTGCGACTCCGACTTTTCTAAAT
GCTGGACGTAAGCGTCGTGGTGAATTTGTTTCTTGTTTCCTTCTACAATTAACTGATGATATGAATTCAATTGGACGAAC
AATTAACTCTGCCTTGCAACTTTCTAAAAATGGTGGTGGTGTTGGTCTAAACTTGACTAATTTACGTGCTGCTGGTGCAC
CTATCAAAGGTTACGATGGAGTGGCAGCTGGTGTCGTTCCTGTCATGAAACTTTTTGAAGACGCCTTTTCTTATGCCAAT
CAATTGGGGCAACGTCAAGGTGCTGGTGTTGTTTATTTATCACTATTCCATCCAGATATTATGGAATTCTTGTCCACTAA
AAAAGAAAATGCGGATGAAAAAATTCGTGTTAAAACACTCTCTCTTGGGGTTACTGTTCCTGATAAATTCTATGAATTGG
TTAAAAAGGGAGAAACAATGTATCTCTTTGAGCCATATTTTGTTGAAAAATATTATGGTAAACCTTTTGCCGAAGTCGAT
ATTACAGCTGAATATGACAAAATGGTAGCCAATCCAGAAATTCGTAAAACATCGATTAATGCCCGTGAACTTGAACAAGA
ACTTTCAAAACTTCAACAAGAATCTGGTTATCCTTACATCGTAAATGTTGACACAGTCAATCAAGCTAACCCTGTTGATG
GTGTAATTTCAATGTCTAACCTTTGTTCTGAAATTTTGCAAGTCCAAACGCCTTCTGTTTTGAACGATGACCAAACTTAT
AAGGTGGTTGGAACTGACGTCGCTTGTAACTTGGGTTCAACTAATGTTTTGAATATGATGACTTCTAGCAATGAATTTGG
AACTTCTATTGAAGCAATGGTTCGCGCTTTAACTTTCATCTCCGAAACTTCAAACCTCGATACTGTTCCAACTGTTCGTA
AAGGTAATGATGAAATGCATGCGATTGGGCTTGGAGCAATGGGCTTACATTCTTTCTTAGCACAAAATCAAATTCATTAT
GACAGCAAAGAAGCCGTTGAATTTACTAGTGTTTATTTCATGTTGTTGAACTATTATACTTTAGTGGCTTCAAATAAATT
AGCTGTTGAAAAAGGGTCTAGCTTCAAGAATTTTGAAAAATCTGCCTATGCTGATGGTTCTTATTTTGATAAATATTTAA
CACATGATTATTTTGCAGCTGTCTCTTCTAAAGTTCAAGAACTTTTCAAAGGGATTGCTGTTCCAACAATCGAGGATTGG
ACTGCTTTACGTGACACAGTAAAAACTTCTGGACTCTATAATTCATACCGTATGGCTGTGGCTCCAAATGGTTCTATTTC
TTATATCAATGACTGTTCTTCTTCTATTCACCCAATTGTCAATCGCATCGAAGAACGTCAAGAAAAGAAAGTTGGGAAAA
TTTATTATCCAGCTGCTGGACTTGCCACTGAAACAATTCCTTATTACAAATCAGCTTATGATACAGATATGCGTGCAGTA
ATTGACGTTTATGCAGCTGCTACAGAACATGTTGACCAAGGTTTGTCACTCACTTTATTTATGCGTTCAACTTTACCTGA
AGGTCTCTATGAATGGAAAGTGGCAAATAATAAAATGACAACTCGTGACTTGTCTATTTTGCGTAACTATGCTTACAAAA
AAGGAATCAAAACAATTTACTACATTCGTACATTTACTGACGACCAAGAAGAAGTCGGTGCTAACCAATGTGAAAGTTGC
GTTATTTAA

Upstream 100 bases:

>100_bases
ATTTTTAACTTTTTATTTCTATTAATTAGTTTGAACTTATTATAATATTAGAGTTCATCCCTCTGTTCTTTATGGTTGAA
CGCTTTAAGAAAGGTCACTT

Downstream 100 bases:

>100_bases
TACAACAGAAGCATTGGTGCTAAAGCATCTATTGCTTCTACGGAAGCCGCTAAGACGGACTTTCTATTTTCTTACTTGTT
TTCATTCATTAAAGTATCAT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFIT
NLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLN
AGRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVD
ITAEYDKMVANPEIRKTSINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTY
KVVGTDVACNLGSTNVLNMMTSSNEFGTSIEAMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSSKVQELFKGIAVPTIEDW
TALRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAV
IDVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
VI

Sequences:

>Translated_722_residues
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFIT
NLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLN
AGRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVD
ITAEYDKMVANPEIRKTSINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTY
KVVGTDVACNLGSTNVLNMMTSSNEFGTSIEAMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSSKVQELFKGIAVPTIEDW
TALRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAV
IDVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
VI
>Mature_721_residues
SLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFITN
LRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNA
GRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYANQ
LGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVDI
TAEYDKMVANPEIRKTSINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYK
VVGTDVACNLGSTNVLNMMTSSNEFGTSIEAMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHYD
SKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSSKVQELFKGIAVPTIEDWT
ALRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVI
DVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESCV
I

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=603, Percent_Identity=26.865671641791, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI1789030, Length=715, Percent_Identity=49.6503496503497, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=569, Percent_Identity=27.768014059754, Blast_Score=182, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17555242, Length=647, Percent_Identity=26.4296754250386, Blast_Score=187, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=614, Percent_Identity=27.0358306188925, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=619, Percent_Identity=27.3021001615509, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17352481, Length=629, Percent_Identity=25.7551669316375, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81496; Mature: 81365

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWL
CCHHHHCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VKEEYLEAEFLEKYKPEFITNLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCH
EDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNAGRKRRGEFVSCFLLQLTDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHH
MNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
HHHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEE
YLFEPYFVEKYYGKPFAEVDITAEYDKMVANPEIRKTSINARELEQELSKLQQESGYPYI
EEECHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCCCEE
VNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYKVVGTDVACNLGSTNVLNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHH
TSSNEFGTSIEAMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
VSSKVQELFKGIAVPTIEDWTALRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHH
NRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVIDVYAAATEHVDQGLSLTLF
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
EHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHC
VI
CC
>Mature Secondary Structure 
SLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWL
CHHHHCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VKEEYLEAEFLEKYKPEFITNLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCH
EDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNAGRKRRGEFVSCFLLQLTDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHH
MNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
HHHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEE
YLFEPYFVEKYYGKPFAEVDITAEYDKMVANPEIRKTSINARELEQELSKLQQESGYPYI
EEECHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCCCEE
VNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYKVVGTDVACNLGSTNVLNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHH
TSSNEFGTSIEAMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
VSSKVQELFKGIAVPTIEDWTALRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHH
NRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVIDVYAAATEHVDQGLSLTLF
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
EHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHC
VI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]