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The map label for this gene is nrdF

Identifier: 15672956

GI number: 15672956

Start: 1001308

End: 1002285

Strand: Reverse

Name: nrdF

Synonym: L0295

Alternate gene names: 15672956

Gene position: 1002285-1001308 (Counterclockwise)

Preceding gene: 15672957

Following gene: 15672952

Centisome position: 42.37

GC content: 34.97

Gene sequence:

>978_bases
ATGTCTGAAACTGAAAATCTTACTGTCCCCACTTATTACAGTGCCATTGACTGGAATTCTATTGAGGATTCTATTGACAA
ATATACTTGGGAAAAATTAACAAGTCAATTTTGGTTAGATACTCGTGTTCCTGTATCTAACGATTTAGATGACTGGCGTA
AACTTCCTCAATTGGAACGTGATACCTTTGCTAAGGCCTTTGCTGGTTTGACATTGCTTGATACTTTGCAATCTGTTGAT
GGTGCTGAAGTGCTCAAACATGATGCAAGAACACCTCAAGAAATTGCCTGTTTCAACAATATCCAATTTATGGAATCTGT
TCATGCAAAGTCTTATTCAACAATTTTTTCAACTCTAAACACGAAAAAAGAAATTGAAGAACTTTTTAATTGGGTAGATA
CCAATGTTTATATGCAAACAAAAGCCGAAATCATCAATAAAATTTATGAATCTGGTACTCCTTTGCAAAAGAAAGTGGCT
TCTGTTTTCCTTGAAACTTGCCTCTTCTACTCTGGTTTCTATACCCCTTTGCGCTATCTTGGAAATAACAAAATGATTAA
CTCAGCGGAAATTATTAAATTAATTATTCGTGATGAGTCAGTTCACGGTACTTATATTGGTTACAAATTCCAACTTGGTT
TTAATGAGTTATCTGAAGATGAGCAAGGTGAATTCCGTGATTGGATGTATGAATTGCTTTATCAACTTTATGAAAATGAA
GAAAAATATTCTCATTTACTTTATGACAAAGTTGGTTGGACTGATGAAGTTTTAACTTTCTTGCGTTATAATGCCAATAA
AGCTTTGATGAATCTTGGACAAGATCCACTTTTCCCTGATACCGCAGCAGACGTTAACCCAGTGGTTATGAATGGAATTT
CAACTTCGTCATCAAACCACGACTTCTTCTCACAAGTTGGTAATTCATACCTTCTTGGTGAAGTGGAAGCCATGTCTGAT
GAAGATTATGAGATCTAA

Upstream 100 bases:

>100_bases
TATGACTAAGCTAAAAATACTGACAGTATTTTCTGTCAGTAAATAAATGAGAGAAAGGAATTGAGTTTGCCCTTTTTAAA
GCAGGCTCATATCTTAAAAT

Downstream 100 bases:

>100_bases
TTAAACAAATTTAAGAGTCTGTCAGTAAATAAGTAAAAACTTATTTACTGACAGCTTTTTTTAGCAAAAAAAGAGAAGAT
TTAACTTCTCTTTTATTTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETENLTVPTYYSAIDWNSIEDSIDKYTWEKLTSQFWLDTRVPVSNDLDDWRKLPQLERDTFAKAFAGLTLLDTLQSVD
GAEVLKHDARTPQEIACFNNIQFMESVHAKSYSTIFSTLNTKKEIEELFNWVDTNVYMQTKAEIINKIYESGTPLQKKVA
SVFLETCLFYSGFYTPLRYLGNNKMINSAEIIKLIIRDESVHGTYIGYKFQLGFNELSEDEQGEFRDWMYELLYQLYENE
EKYSHLLYDKVGWTDEVLTFLRYNANKALMNLGQDPLFPDTAADVNPVVMNGISTSSSNHDFFSQVGNSYLLGEVEAMSD
EDYEI

Sequences:

>Translated_325_residues
MSETENLTVPTYYSAIDWNSIEDSIDKYTWEKLTSQFWLDTRVPVSNDLDDWRKLPQLERDTFAKAFAGLTLLDTLQSVD
GAEVLKHDARTPQEIACFNNIQFMESVHAKSYSTIFSTLNTKKEIEELFNWVDTNVYMQTKAEIINKIYESGTPLQKKVA
SVFLETCLFYSGFYTPLRYLGNNKMINSAEIIKLIIRDESVHGTYIGYKFQLGFNELSEDEQGEFRDWMYELLYQLYENE
EKYSHLLYDKVGWTDEVLTFLRYNANKALMNLGQDPLFPDTAADVNPVVMNGISTSSSNHDFFSQVGNSYLLGEVEAMSD
EDYEI
>Mature_324_residues
SETENLTVPTYYSAIDWNSIEDSIDKYTWEKLTSQFWLDTRVPVSNDLDDWRKLPQLERDTFAKAFAGLTLLDTLQSVDG
AEVLKHDARTPQEIACFNNIQFMESVHAKSYSTIFSTLNTKKEIEELFNWVDTNVYMQTKAEIINKIYESGTPLQKKVAS
VFLETCLFYSGFYTPLRYLGNNKMINSAEIIKLIIRDESVHGTYIGYKFQLGFNELSEDEQGEFRDWMYELLYQLYENEE
KYSHLLYDKVGWTDEVLTFLRYNANKALMNLGQDPLFPDTAADVNPVVMNGISTSSSNHDFFSQVGNSYLLGEVEAMSDE
DYEI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=311, Percent_Identity=49.1961414790997, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788567, Length=333, Percent_Identity=24.6246246246246, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37615; Mature: 37484

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETENLTVPTYYSAIDWNSIEDSIDKYTWEKLTSQFWLDTRVPVSNDLDDWRKLPQLER
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHH
DTFAKAFAGLTLLDTLQSVDGAEVLKHDARTPQEIACFNNIQFMESVHAKSYSTIFSTLN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHC
TKKEIEELFNWVDTNVYMQTKAEIINKIYESGTPLQKKVASVFLETCLFYSGFYTPLRYL
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNNKMINSAEIIKLIIRDESVHGTYIGYKFQLGFNELSEDEQGEFRDWMYELLYQLYENE
CCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHCH
EKYSHLLYDKVGWTDEVLTFLRYNANKALMNLGQDPLFPDTAADVNPVVMNGISTSSSNH
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCC
DFFSQVGNSYLLGEVEAMSDEDYEI
HHHHHCCCCEEEEEHHHCCCCCCCC
>Mature Secondary Structure 
SETENLTVPTYYSAIDWNSIEDSIDKYTWEKLTSQFWLDTRVPVSNDLDDWRKLPQLER
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHH
DTFAKAFAGLTLLDTLQSVDGAEVLKHDARTPQEIACFNNIQFMESVHAKSYSTIFSTLN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHC
TKKEIEELFNWVDTNVYMQTKAEIINKIYESGTPLQKKVASVFLETCLFYSGFYTPLRYL
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNNKMINSAEIIKLIIRDESVHGTYIGYKFQLGFNELSEDEQGEFRDWMYELLYQLYENE
CCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHCH
EKYSHLLYDKVGWTDEVLTFLRYNANKALMNLGQDPLFPDTAADVNPVVMNGISTSSSNH
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCC
DFFSQVGNSYLLGEVEAMSDEDYEI
HHHHHCCCCEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]