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The map label for this gene is tra1077E

Identifier: 15672806

GI number: 15672806

Start: 837114

End: 837932

Strand: Reverse

Name: tra1077E

Synonym: L0470

Alternate gene names: 15672806

Gene position: 837932-837114 (Counterclockwise)

Preceding gene: 15672807

Following gene: 15672805

Centisome position: 35.42

GC content: 34.55

Gene sequence:

>819_bases
TTGAAAGTTACAGGATTCCCTAAAGCCACTTATTATTATTGGGTTAACTGTTTTGAACGGGTAAACAAAGATGAGCTTAT
CGAAAAAGAAATGCTTAAAATACGTCAAGAACATGCCAATGCAGGTTATCGTCCAATGCGTGAATTACTTAAGCAACGTG
GCTATCACGTCAACCACAAGAAGGTACAGCGCTTAATGAAGAAGCTAGGGCTTCGTGTGACGTCTTATTGGCACAAATCA
CGTAAATATAATTCATATAAAGGAAAAGTAGGGACGGTAGCTAAAAATAAATTGCACAGACGATTCAGAACTTCCATTCC
TCATCAAAAAATCACAACAGATACGACAGAATTCAAATATTATGAAGATGGAATTCAGAAAAAGTGTTATCTCAATCCTT
ACATTGATTTATTTAATAGCGAGGTGATTAGTTATCATATCTCTAAACAACCCTCTTATCAATCAATTGATATTGCTCTA
AATCAAGCCTTAGCTGTGACATCTGATTGTCCTTATCGACGTACTTTCCACTCAGATCAAGGATGGGGATACCAAATGAG
AGACTATGTTTCTAAATTAAAATCTCATAGAATTTTTCAGTCTATGAGCCGTAAAGGAAACTGTCATGATAATTCAGTTA
TGGAGAATTTCTTTGGGCTACTTAAACAAGAAATTTACTACGGACATATCTTCTCGTCTTTCGAAGAACTTGAACAGGTT
ATTGTAATCTGGATTAGGTACTATAATACAAAACGAATCAAACAAAAATTGAACTGGATGAGTCCAATTCAATTTCGCTT
GAATTACCAAAACAATTAA

Upstream 100 bases:

>100_bases
CTTATTTAAAAGGACTTCGGAGATTGGAGAAAAATGCTCTAATGAACAAAAATCAAGACTCATCTACAGTCTCCGTAAAA
CCTTCAAACTCAAAGAAATC

Downstream 100 bases:

>100_bases
AAAAATCCAAGTATTTTGGAAAGGTTATAATAAACTAGACACAAAGTTAAGAGAAATCGTGGAAAGGTTTATTATGGCAA
GAAGAAAATTCGATAAACAA

Product: IS1077E transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Sequences:

>Translated_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN
>Mature_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIDLFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=268, Percent_Identity=41.7910447761194, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788405, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787263, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786753, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786570, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786490, Length=275, Percent_Identity=29.4545454545455, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32857; Mature: 32857

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]