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The map label for this gene is exoA

Identifier: 15672773

GI number: 15672773

Start: 798032

End: 799036

Strand: Reverse

Name: exoA

Synonym: L198056

Alternate gene names: 15672773

Gene position: 799036-798032 (Counterclockwise)

Preceding gene: 15672791

Following gene: 15672772

Centisome position: 33.78

GC content: 37.91

Gene sequence:

>1005_bases
TTGAGAAGATTCTCATCTAAATATTATACCAAAAAACCTCTAAAATCGCATTGTCTGATTAAGTTTGTTATAATTATCAT
AGAAAAGTTAAGAATTCCTTTAAGTCACAGCTCTTTGGAAATTCTTTTAAAAAATAAAGGAACGATTATGAACTATAAAT
TTATCTCATGGAATATTGACTCACTCAATGCTGCATTGACTGGAACCTCAGAACGTGCTGCCCTTTCTCTTGCTGTTGTT
AAAAATCTTGCCCAAGCACAACCAGACGTTTTAGCCATTCAAGAAACCAAACTTCCTTCTACTGGACCTAAAAAAGCTCA
TTTGGAAGTTTTGGAAGAACTGTTTAAAGGTTATAAAATCGTCTGGCGTTCATCAGTAGAGCCTGCTCGTAAAGGATATT
CTGGAACAATGATTCTTTATAAAGAAACTCTACCAGAACCAGTCATCACTTTTCCTGAAATTGATGCTCCTGAACCTATG
GATGCCGAAGGTCGAATCATCACCCTTGAATTTCCTGATTTCTTTGTCACTACGGTTTATACACCAAATGCGCAAGAAGG
CCTGACACGACTTGATTTACGTGGAATTTGGGATGATAACTATCGTAACTATCTCACAAGCCTTGATGCTCAAAAACCTG
TCTTTGCTTGTGGAGATTTCAATGCGGCCTATACAGAAATTGACTTAGCCAATCCCCGTGGTAATCACAACTCAGCTGGA
TTTACTGACCAAGAACGTGAAAAATTTGGTCAACTTTTAGAGGCTGGATTTACTGATACCTTTCGTCAACTCCATGGTCA
AGTTGAGAAATTTTATGAAGAAGGACGTAGTATTTATACTTGGTTTGCCCAACGTGCAAAAACTTCAAAACTAAATAATT
CAGGTTGGAGAATTGACTACTGGCTGGTTTCCAATCGTCTTGCCCAAGCAGTTAAAGTTTCTGAACCCCTTGATTCTGGT
GAACGTTTAGACCACGTCCCCATTCTCTTAGAATTTGAACTCTAA

Upstream 100 bases:

>100_bases
AAGATGCAACTTGCCTGATGGATAATAAATTGGGGTTGTAATGTAAAAAGTTTTGTTTTCTGTCATATTTTCCTCTTTCC
GAGCAAATGAGGCTCGATAT

Downstream 100 bases:

>100_bases
AATAAAAGAACCTTAAAGGTTCTTTTTTATTGTCAATAAAACAAATAGAGACTATAATTGAGTAAAAATAGTTGAGGAGA
ACATGTTTATGGTTAATATA

Product: exodeoxyribonuclease A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRRFSSKYYTKKPLKSHCLIKFVIIIIEKLRIPLSHSSLEILLKNKGTIMNYKFISWNIDSLNAALTGTSERAALSLAVV
KNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPARKGYSGTMILYKETLPEPVITFPEIDAPEPM
DAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDLANPRGNHNSAG
FTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTSKLNNSGWRIDYWLVSNRLAQAVKVSEPLDSG
ERLDHVPILLEFEL

Sequences:

>Translated_334_residues
MRRFSSKYYTKKPLKSHCLIKFVIIIIEKLRIPLSHSSLEILLKNKGTIMNYKFISWNIDSLNAALTGTSERAALSLAVV
KNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPARKGYSGTMILYKETLPEPVITFPEIDAPEPM
DAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDLANPRGNHNSAG
FTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTSKLNNSGWRIDYWLVSNRLAQAVKVSEPLDSG
ERLDHVPILLEFEL
>Mature_334_residues
MRRFSSKYYTKKPLKSHCLIKFVIIIIEKLRIPLSHSSLEILLKNKGTIMNYKFISWNIDSLNAALTGTSERAALSLAVV
KNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPARKGYSGTMILYKETLPEPVITFPEIDAPEPM
DAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDLANPRGNHNSAG
FTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTSKLNNSGWRIDYWLVSNRLAQAVKVSEPLDSG
ERLDHVPILLEFEL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=289, Percent_Identity=35.9861591695502, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI18375503, Length=289, Percent_Identity=35.9861591695502, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI18375501, Length=289, Percent_Identity=35.9861591695502, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788046, Length=298, Percent_Identity=29.1946308724832, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71989536, Length=285, Percent_Identity=35.0877192982456, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71989539, Length=165, Percent_Identity=29.6969696969697, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330655, Length=286, Percent_Identity=34.6153846153846, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI17136678, Length=286, Percent_Identity=34.6153846153846, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 38077; Mature: 38077

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFSSKYYTKKPLKSHCLIKFVIIIIEKLRIPLSHSSLEILLKNKGTIMNYKFISWNID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEECC
SLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKI
CCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEE
VWRSSVEPARKGYSGTMILYKETLPEPVITFPEIDAPEPMDAEGRIITLEFPDFFVTTVY
EEECCCCHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCEEEEEEE
TPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDLANPRGNHNSAG
CCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCC
FTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTSKLNNSGWRIDY
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEE
WLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL
EEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRFSSKYYTKKPLKSHCLIKFVIIIIEKLRIPLSHSSLEILLKNKGTIMNYKFISWNID
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEEEECC
SLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKI
CCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEE
VWRSSVEPARKGYSGTMILYKETLPEPVITFPEIDAPEPMDAEGRIITLEFPDFFVTTVY
EEECCCCHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEECCCEEEEEEE
TPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDLANPRGNHNSAG
CCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCC
FTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTSKLNNSGWRIDY
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEE
WLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL
EEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]