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The map label for this gene is fadD

Identifier: 15672644

GI number: 15672644

Start: 654526

End: 656049

Strand: Reverse

Name: fadD

Synonym: L54546

Alternate gene names: 15672644

Gene position: 656049-654526 (Counterclockwise)

Preceding gene: 15672655

Following gene: 15672643

Centisome position: 27.73

GC content: 37.27

Gene sequence:

>1524_bases
TTGAAATTCAAACACGAACCCCTAAATTTGTATCTAAACTTTAAAAAATCAGCTGAACAGTTTCCTGATAGCCCAATTAT
CTTTGATGAGGCTGTTGGAGCTTTCCCCGAACTAGCTCTTGAAACGACTTACCAAAAGACTCATCAAGCAATTGTTGATA
AGGCAAGTCTCTTATCTGAGTTGGGAATCAAAAAATCGGATAAAGTCATTATTTTTAAATCAAGTGCTTTTGATACCTAT
CTTTTAGCTGTTGCTGTTTCTTATCTAGGCGCTGTTCCAGCAATGATTTCTTATCATCTTCCTGCCGAAACAATGGATAT
TTTAGCTGGACGTTTAGGCAATCCTTGGCTCATTTATGACCAAGTTACTGACAAAACTGTAAATGAAATGAAGAATCTTG
AAGATTCTAAAAAACTTTCTGTCAGTAAATTTGCTTTGGAAAAACCTACTCAAATTGCTGACAGTCAGCAATTGGCCAGT
GATGAGATTGCTTATCTCACTCATACTTCTGGAACAACTGGTGTACCAAAATTAATCGCTCACTCTGCTAATTCAATGGG
TTGGCGTTGGGTGCTTCAAAGAACTGTAATGGATTGGATGCCTGATAAGGAAGAAATTTTAGCTTTCCATATTTCTCCTG
TTCATTCTCGTTTCAATATAGGGGTTTCATCAGCAATGACCTTTGGGTTTGGCCTTATGCCCCTCAATGATTTATCAAAA
GATAATCTTGTCAATCTTTTTGCTAAACATCAGCCTTATGCTTTTGAAACACATCCAAACGATTTTGTTCGTCTAGCGAA
TCTTGCAAAGCAAGAACCTGAGATTTTTGCTTCAATTCGTTACCTTCACTCAACTTTTGATGCAATTAATAAAGAAACAA
TGCACACTTTTCTTTCTGCCTCTAAGCAAAAAGATCCAGTCTTCCTACAAATTTATGGACAATCAGAATGTGGCCCAATG
ATTTGGAAAAAGCACCGTTTATCAACTTTAGCAGACACTAATGCCCGTGAAATGGGGATTGGTATGCCTGGATTATCCAA
AGCACGTATTGCCGATGAAGATGGAAATGAATGTCCTGCAGGTACGCCTGGAAGAATCCACTTCCTCTCAAAAGGAAGAG
CTTTGACTTACTATAAAGAAGAAGAACGTTTTAATAAAGAAGTCTATGGTGACTGGTGGGATACTGGCGATTGGGGTCTC
ATGAATGAAGACGGAATTCTCTTTTTACATGACAGACAAGTTGATTTAATTGATAAAATTGAATCTAATTTGGCAATTGA
AGATCTTCTGTTAGACCAACACGACTTCTTAGATGAAGTTGTGATTATTCGTGATAAATCTGGTAAACCACAACCTATTT
TGGCTCTAGCTGAAAATGCGGAAATGGATTGGTCAGCTTGGTGGGCTTCAATTGTTGATTTGCCATTTTTAAATGAACCT
ATTCTTATGGCTTACGATGAAATTCCTCGTACAGCAACAATGAAAGTTCAACGATTAGCTCTTGAAAGAGAATTAGCTAA
ATAA

Upstream 100 bases:

>100_bases
AGTATTTTATTCTCTACGACTTAAGAAAACATTTACATTAACTATTGGTCTAAAGAATACTTTGTGATATAATAAACTTA
TTAACTTGAGGAGATTTTTT

Downstream 100 bases:

>100_bases
GTGTTCCTAATTTTACTGACAGAACTGTCAGCCACTTATTCATCAGCAAATTTATTATCAAAACTACTGACAAAACTGTC
AGTAGTTTTTTTGCTTAAAA

Product: long-chain acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; Acyl-CoA synthetase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKFKHEPLNLYLNFKKSAEQFPDSPIIFDEAVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLGNPWLIYDQVTDKTVNEMKNLEDSKKLSVSKFALEKPTQIADSQQLAS
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKEEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSK
DNLVNLFAKHQPYAFETHPNDFVRLANLAKQEPEIFASIRYLHSTFDAINKETMHTFLSASKQKDPVFLQIYGQSECGPM
IWKKHRLSTLADTNAREMGIGMPGLSKARIADEDGNECPAGTPGRIHFLSKGRALTYYKEEERFNKEVYGDWWDTGDWGL
MNEDGILFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALAENAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERELAK

Sequences:

>Translated_507_residues
MKFKHEPLNLYLNFKKSAEQFPDSPIIFDEAVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLGNPWLIYDQVTDKTVNEMKNLEDSKKLSVSKFALEKPTQIADSQQLAS
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKEEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSK
DNLVNLFAKHQPYAFETHPNDFVRLANLAKQEPEIFASIRYLHSTFDAINKETMHTFLSASKQKDPVFLQIYGQSECGPM
IWKKHRLSTLADTNAREMGIGMPGLSKARIADEDGNECPAGTPGRIHFLSKGRALTYYKEEERFNKEVYGDWWDTGDWGL
MNEDGILFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALAENAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERELAK
>Mature_507_residues
MKFKHEPLNLYLNFKKSAEQFPDSPIIFDEAVGAFPELALETTYQKTHQAIVDKASLLSELGIKKSDKVIIFKSSAFDTY
LLAVAVSYLGAVPAMISYHLPAETMDILAGRLGNPWLIYDQVTDKTVNEMKNLEDSKKLSVSKFALEKPTQIADSQQLAS
DEIAYLTHTSGTTGVPKLIAHSANSMGWRWVLQRTVMDWMPDKEEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSK
DNLVNLFAKHQPYAFETHPNDFVRLANLAKQEPEIFASIRYLHSTFDAINKETMHTFLSASKQKDPVFLQIYGQSECGPM
IWKKHRLSTLADTNAREMGIGMPGLSKARIADEDGNECPAGTPGRIHFLSKGRALTYYKEEERFNKEVYGDWWDTGDWGL
MNEDGILFLHDRQVDLIDKIESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALAENAEMDWSAWWASIVDLPFLNEP
ILMAYDEIPRTATMKVQRLALERELAK

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=369, Percent_Identity=24.9322493224932, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=23.1168831168831, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57349; Mature: 57349

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKHEPLNLYLNFKKSAEQFPDSPIIFDEAVGAFPELALETTYQKTHQAIVDKASLLSE
CCCCCCCEEEEEEECHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGIKKSDKVIIFKSSAFDTYLLAVAVSYLGAVPAMISYHLPAETMDILAGRLGNPWLIYD
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEE
QVTDKTVNEMKNLEDSKKLSVSKFALEKPTQIADSQQLASDEIAYLTHTSGTTGVPKLIA
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCHHHHCCCCEEEEEECCCCCCHHHHHH
HSANSMGWRWVLQRTVMDWMPDKEEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSK
HCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHCCCHHHHHHHCCCCCCCCCCCC
DNLVNLFAKHQPYAFETHPNDFVRLANLAKQEPEIFASIRYLHSTFDAINKETMHTFLSA
CHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKQKDPVFLQIYGQSECGPMIWKKHRLSTLADTNAREMGIGMPGLSKARIADEDGNECPA
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEECCCCCCCCCC
GTPGRIHFLSKGRALTYYKEEERFNKEVYGDWWDTGDWGLMNEDGILFLHDRQVDLIDKI
CCCCEEEEEECCCEEEEECHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
ESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALAENAEMDWSAWWASIVDLPFLNEP
HHCCHHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCC
ILMAYDEIPRTATMKVQRLALERELAK
CEEEHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFKHEPLNLYLNFKKSAEQFPDSPIIFDEAVGAFPELALETTYQKTHQAIVDKASLLSE
CCCCCCCEEEEEEECHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGIKKSDKVIIFKSSAFDTYLLAVAVSYLGAVPAMISYHLPAETMDILAGRLGNPWLIYD
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEE
QVTDKTVNEMKNLEDSKKLSVSKFALEKPTQIADSQQLASDEIAYLTHTSGTTGVPKLIA
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCHHHHCCCCEEEEEECCCCCCHHHHHH
HSANSMGWRWVLQRTVMDWMPDKEEILAFHISPVHSRFNIGVSSAMTFGFGLMPLNDLSK
HCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHCCCHHHHHHHCCCCCCCCCCCC
DNLVNLFAKHQPYAFETHPNDFVRLANLAKQEPEIFASIRYLHSTFDAINKETMHTFLSA
CHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKQKDPVFLQIYGQSECGPMIWKKHRLSTLADTNAREMGIGMPGLSKARIADEDGNECPA
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEECCCCCCCCCC
GTPGRIHFLSKGRALTYYKEEERFNKEVYGDWWDTGDWGLMNEDGILFLHDRQVDLIDKI
CCCCEEEEEECCCEEEEECHHHHHCHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
ESNLAIEDLLLDQHDFLDEVVIIRDKSGKPQPILALAENAEMDWSAWWASIVDLPFLNEP
HHCCHHHHHHHCCHHCCCCEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCC
ILMAYDEIPRTATMKVQRLALERELAK
CEEEHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]