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The map label for this gene is dnaG

Identifier: 15672534

GI number: 15672534

Start: 544010

End: 545923

Strand: Direct

Name: dnaG

Synonym: L0269

Alternate gene names: 15672534

Gene position: 544010-545923 (Clockwise)

Preceding gene: 15672533

Following gene: 15672535

Centisome position: 23.0

GC content: 33.86

Gene sequence:

>1914_bases
ATGGTTTCACTTGACACTGAGGTTGTTAATGATTTAAAGTCAAAAGTTAATATTGCTGATTTAATTTCACAGTATGTTGC
TTTAAGTCGTACAGGTAAGAATTATATTGGTTTATGCCCTTTTCATGGTGAGAAAACTCCTTCCTTTAATGTAAATGCTG
AAAAAGGTTTTTATCATTGTTTTGGTTGTGGGCGCTCTGGTGATGCGATTGAGTTTTTAAAAGAATACAATCAAGTTGGA
TTCGTTGATGCTGTTAAGGAACTGGCTGATTTTGCAGGAGTTACTCTTAACATTTCTGATGATAGAGAAGAAAAAAATAA
TCCAAATGCACCATTGTTTGAAATCAATAACCAGGCTGCTCGTTTATACAATATTCTATTGATGTCAACGGAGTTGGGTG
AACGGGCTAGGAAATATCTTGAGGAGCGGGGGATTACTGATGATGTAATCAAGAGATTTAATATTGGTTTAGCTCCTGAA
GAAAATGATTTTATTTTTAAAAATTTATCAAATAAGTTTGATGAAGAAATTATGGCAAAGTCTGGTTTATTTCATTTCTC
AAATAATAACGTTTTTGATGCTTTTACGAATCGAATCATGTTTCCAATCACCAACGAATATGGACATACAATTGGTTTTT
CTGGACGTAAATGGCAGGAAAATGATGATTCTAAAGCCAAATATATTAATACTTCAGCAACAACTATATTTGATAAATCT
TATGAACTTTGGAATTTAGATAAAGCAAAACCAACAATTTCAAAGCAGCATGAAGTCTATTTAATGGAAGGTTTTATGGA
TGTTATTGCGGCTTATAAAGCTGGAATAAATAATGTTGTTGCCTCAATGGGGACGGCTTTAACCGAAAAACACATCCGTC
GTTTGAAACAAATGGCAAAAAAATTCGTATTAGTTTATGATGGAGATTCTGCTGGGCAAAATGCAATTTATAAGGCCATT
GATTTGATTGGTGAATCTGCTGTGCAGATTGTAAAGGTTCCGGAAGGACTTGATCCAGATGAGTATTCTAAAAATTATGG
TTTGAAGGGATTATCTGCCTTAATGGAAACGGGGCGTATTCAACCCATTGAATTTTTAATTGATTATTTGAGACCTGAAA
ACCTTGCTAATTTACAAACGCAACTTGATTTTATTGAACAGATTTCACCAATGATTGCAAAGCTTCCATCGATTACGGCT
CAGGATGCCTATATAAGAAAATTAGTTGAGATTTTGCCTGATTTCGAGTATAATCAAGTGGAGCAGGCTGTAAATTTACG
GCGTGAAAATATGACAATTACTGACCATCCTGTCAGTAATTTAGATGCTTCAAGTTTGACCGAAAGTTTTACTGACGAAA
ATGATTATTCTAGTCTTGAATCAGTCATGCCTGTAGATTTTGAGGAGGCTTATTATGAGAATAATGTTAAAACTCAACAA
ACTTACAGAAGAAGTGAATCGGCTCAAGTTGTTCAACCATCTGTTCAGGTTCCTAAGTTGTCACGTTCGGAAAAAGCCGA
AGAAATGCTACTCCATCGAATGATTTATCATTCATCAGTTTTGAAAAAATTTTCTCAAGATGAAAATTTTAGATTCGTCC
ATAAACGTTATCAGGATTTATTTGATAAAATCTTGCTTGAAGCAATGGTTTATGAACAAATTGATGAATCACACTTAGCA
AGTGAGCTGTCAGACGAAGAACGTAGTCTGTTTTACCAGATTATAAGTTTAGATTTGCCTGATACAGCATCAAGTCAGGA
AATCAAAGACTTAGTTTCCATTTTTTCTAATGAAATGGAACAAATAAAATTTGAAGAACTCATTCAACAGTTGGCAACTG
CTGAGAAAGCAGGAAACAAAGAACGTGTCTTAGAGTTAACCCTTCAGATTATTAACCAAAAGAAAAAATTATGA

Upstream 100 bases:

>100_bases
TATTTTAGAAAATGAATGATGATTAAGAACCTTTCTGAAGTGTTATACTTATTTTATGAGATTACTTTTATGATTAATCG
TAGGGAAGAAGGGGGAGATT

Downstream 100 bases:

>100_bases
TAATTTTTTACTGACAATTGGCTAGTCTATTTAATGACAGTTTTGTCAGTAGTTTTACCCAAAGAGGAGGAGCGAATTGG
CAGCAAATAAAAAAAGAATA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MVSLDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FVDAVKELADFAGVTLNISDDREEKNNPNAPLFEINNQAARLYNILLMSTELGERARKYLEERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEIMAKSGLFHFSNNNVFDAFTNRIMFPITNEYGHTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKQHEVYLMEGFMDVIAAYKAGINNVVASMGTALTEKHIRRLKQMAKKFVLVYDGDSAGQNAIYKAI
DLIGESAVQIVKVPEGLDPDEYSKNYGLKGLSALMETGRIQPIEFLIDYLRPENLANLQTQLDFIEQISPMIAKLPSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMTITDHPVSNLDASSLTESFTDENDYSSLESVMPVDFEEAYYENNVKTQQ
TYRRSESAQVVQPSVQVPKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDLFDKILLEAMVYEQIDESHLA
SELSDEERSLFYQIISLDLPDTASSQEIKDLVSIFSNEMEQIKFEELIQQLATAEKAGNKERVLELTLQIINQKKKL

Sequences:

>Translated_637_residues
MVSLDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FVDAVKELADFAGVTLNISDDREEKNNPNAPLFEINNQAARLYNILLMSTELGERARKYLEERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEIMAKSGLFHFSNNNVFDAFTNRIMFPITNEYGHTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKQHEVYLMEGFMDVIAAYKAGINNVVASMGTALTEKHIRRLKQMAKKFVLVYDGDSAGQNAIYKAI
DLIGESAVQIVKVPEGLDPDEYSKNYGLKGLSALMETGRIQPIEFLIDYLRPENLANLQTQLDFIEQISPMIAKLPSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMTITDHPVSNLDASSLTESFTDENDYSSLESVMPVDFEEAYYENNVKTQQ
TYRRSESAQVVQPSVQVPKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDLFDKILLEAMVYEQIDESHLA
SELSDEERSLFYQIISLDLPDTASSQEIKDLVSIFSNEMEQIKFEELIQQLATAEKAGNKERVLELTLQIINQKKKL
>Mature_637_residues
MVSLDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHCFGCGRSGDAIEFLKEYNQVG
FVDAVKELADFAGVTLNISDDREEKNNPNAPLFEINNQAARLYNILLMSTELGERARKYLEERGITDDVIKRFNIGLAPE
ENDFIFKNLSNKFDEEIMAKSGLFHFSNNNVFDAFTNRIMFPITNEYGHTIGFSGRKWQENDDSKAKYINTSATTIFDKS
YELWNLDKAKPTISKQHEVYLMEGFMDVIAAYKAGINNVVASMGTALTEKHIRRLKQMAKKFVLVYDGDSAGQNAIYKAI
DLIGESAVQIVKVPEGLDPDEYSKNYGLKGLSALMETGRIQPIEFLIDYLRPENLANLQTQLDFIEQISPMIAKLPSITA
QDAYIRKLVEILPDFEYNQVEQAVNLRRENMTITDHPVSNLDASSLTESFTDENDYSSLESVMPVDFEEAYYENNVKTQQ
TYRRSESAQVVQPSVQVPKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDLFDKILLEAMVYEQIDESHLA
SELSDEERSLFYQIISLDLPDTASSQEIKDLVSIFSNEMEQIKFEELIQQLATAEKAGNKERVLELTLQIINQKKKL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=411, Percent_Identity=33.8199513381995, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_LACLA (Q04505)

Other databases:

- EMBL:   AE005176
- EMBL:   D14690
- EMBL:   D10168
- PIR:   H86693
- PIR:   JC2485
- RefSeq:   NP_266708.1
- ProteinModelPortal:   Q04505
- GeneID:   1114171
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0269
- NMPDR:   fig|272623.1.peg.567
- HOGENOM:   HBG573408
- OMA:   RNYLYQR
- ProtClustDB:   PRK05667
- BioCyc:   LLAC272623:L0269-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 72752; Mature: 72752

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHC
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
FGCGRSGDAIEFLKEYNQVGFVDAVKELADFAGVTLNISDDREEKNNPNAPLFEINNQAA
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCHHH
RLYNILLMSTELGERARKYLEERGITDDVIKRFNIGLAPEENDFIFKNLSNKFDEEIMAK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
SGLFHFSNNNVFDAFTNRIMFPITNEYGHTIGFSGRKWQENDDSKAKYINTSATTIFDKS
CCCEEECCCCEEHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHEEECCC
YELWNLDKAKPTISKQHEVYLMEGFMDVIAAYKAGINNVVASMGTALTEKHIRRLKQMAK
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFVLVYDGDSAGQNAIYKAIDLIGESAVQIVKVPEGLDPDEYSKNYGLKGLSALMETGRI
HEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCHHHHHHHHHCCCC
QPIEFLIDYLRPENLANLQTQLDFIEQISPMIAKLPSITAQDAYIRKLVEILPDFEYNQV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHH
EQAVNLRRENMTITDHPVSNLDASSLTESFTDENDYSSLESVMPVDFEEAYYENNVKTQQ
HHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCCCHHHH
TYRRSESAQVVQPSVQVPKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDL
HHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FDKILLEAMVYEQIDESHLASELSDEERSLFYQIISLDLPDTASSQEIKDLVSIFSNEME
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QIKFEELIQQLATAEKAGNKERVLELTLQIINQKKKL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSLDTEVVNDLKSKVNIADLISQYVALSRTGKNYIGLCPFHGEKTPSFNVNAEKGFYHC
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEE
FGCGRSGDAIEFLKEYNQVGFVDAVKELADFAGVTLNISDDREEKNNPNAPLFEINNQAA
EECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCHHH
RLYNILLMSTELGERARKYLEERGITDDVIKRFNIGLAPEENDFIFKNLSNKFDEEIMAK
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
SGLFHFSNNNVFDAFTNRIMFPITNEYGHTIGFSGRKWQENDDSKAKYINTSATTIFDKS
CCCEEECCCCEEHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCHHEEECCC
YELWNLDKAKPTISKQHEVYLMEGFMDVIAAYKAGINNVVASMGTALTEKHIRRLKQMAK
CCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFVLVYDGDSAGQNAIYKAIDLIGESAVQIVKVPEGLDPDEYSKNYGLKGLSALMETGRI
HEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCHHHHHHHHHCCCC
QPIEFLIDYLRPENLANLQTQLDFIEQISPMIAKLPSITAQDAYIRKLVEILPDFEYNQV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHH
EQAVNLRRENMTITDHPVSNLDASSLTESFTDENDYSSLESVMPVDFEEAYYENNVKTQQ
HHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCCCHHHH
TYRRSESAQVVQPSVQVPKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDL
HHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FDKILLEAMVYEQIDESHLASELSDEERSLFYQIISLDLPDTASSQEIKDLVSIFSNEME
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QIKFEELIQQLATAEKAGNKERVLELTLQIINQKKKL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808