Click here to switch to the map view.

The map label for this gene is ydiB

Identifier: 15672361

GI number: 15672361

Start: 383134

End: 384354

Strand: Direct

Name: ydiB

Synonym: L183112

Alternate gene names: 15672361

Gene position: 383134-384354 (Clockwise)

Preceding gene: 15672360

Following gene: 15672362

Centisome position: 16.2

GC content: 37.1

Gene sequence:

>1221_bases
ATGACTGTATTATATGATGAAATCATGGTTCGTTATGGTGAACTTTCAACCAAAGGAAAAAATCGTGGTTACTTTATCAA
TCGTTTGGCAAAAAATATCCGCGAAGTCCTTGCAGATATGCCTGAATTAAAAATTACAGCAGTAAGAGATAGAGCACACA
TTGAACTTAATGGAGCAGATTATGCTGAAGTTTCAAATCGTTTAACAAAAGTGTTTGGAATCCAAAATTTTTCACCTTCA
ATCAAAGTCGAAAAATCAATGCCCATTATCAAAGAAGAAGTTGTGGCACTTTTTAAGGAGATTTATAGCGAAGGGCAGAC
TTTTAAAATTGCTACCAGACGAGCTGACCATAACTTTGAATTAGATTCAACAGAATTAAATATTGCTTTAGGAGATGTTG
TTTTTGATAATTTCTCTTACGCAAAAGTACAAATGAAAAAGCCTGATATCACATTGCGGGCTGAAATCAGACAAGATGCG
GCTTATCTTAGTTTTGAAGATATAAAAGGTGCTGGAGGAATGCCAGTCGGAACAGCAGGACGTGGACATCTAATGCTCTC
AGGTGGAATTGATTCGCCAGTTGCAGGTTATTTGGCGTTGAAACGCGGAGTTGAGATTGAGGCTGTTCACTTTGCTAGCC
CTCCTTACACTTCACCAGGAGCTTTGAAGAAAGCCAAAGACTTAGCAGCTAAATTAACCGTTTTTGGAGGTTCAATTACC
TTTATTGAAGTACCATTTACCGAGATTCAAGAAGAAATAAAAGCCAAGGCTCCACAAGCATATTTGATGACAATTACACG
CCGTTTTATGATGCGCGTGGTTGATCGTGTTCGTGAAGAACGTGGTGGAAAAGTGATTATTAATGGAGAATCTTTGGGAC
AAGTTGCTAGCCAAACGCTTGGCTCAATGTCTGCCATTAATGAAGTAACTAATACTCCAGTTATTCGACCAGTAGTAACA
ATGGATAAAAATGAAATTATTGAAATAGCTGAAAAAATCGATACTTTTAATTTATCAATTCTACCATTTGAAGATTGTTG
TACTGTCTTTGCACCACCTTCTCCAAAAACAAATCCAAAACTTGAAAATTGTATTCAATATGAAAAGAGAATGGATGTTG
AGGGGATGGTTGACCGTGCGGTCAAAGGAATTATGCTCACAACAATTATTGGTGAAAACTGGGACCAAACTGAAGAAGAG
GAATTTGCTGATTTCTTATAA

Upstream 100 bases:

>100_bases
TTTAAATAAAGAAGATTTGACTGGTTTTTTGACTTAAATCATTCATTTTTTTGAAGTATTTAATGATTAAAAGTATGCAA
AAGAATATAGGAGAACAAAA

Downstream 100 bases:

>100_bases
TAAAAAATGCTACTTATAATATTATGAGTAGCATTTTCATTTGATTAGGAAACTACGATTGATAGTCTAAACTTGACAAA
GATTGGAAACAAGGATATAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPS
IKVEKSMPIIKEEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRAEIRQDA
AYLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGSIT
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVT
MDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIIGENWDQTEEE
EFADFL

Sequences:

>Translated_406_residues
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPS
IKVEKSMPIIKEEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRAEIRQDA
AYLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGSIT
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVT
MDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIIGENWDQTEEE
EFADFL
>Mature_405_residues
TVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGADYAEVSNRLTKVFGIQNFSPSI
KVEKSMPIIKEEVVALFKEIYSEGQTFKIATRRADHNFELDSTELNIALGDVVFDNFSYAKVQMKKPDITLRAEIRQDAA
YLSFEDIKGAGGMPVGTAGRGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGSITF
IEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTLGSMSAINEVTNTPVIRPVVTM
DKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPKLENCIQYEKRMDVEGMVDRAVKGIMLTTIIGENWDQTEEEE
FADFL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=357, Percent_Identity=29.1316526610644, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_LACLA (Q9CII1)

Other databases:

- EMBL:   AE005176
- PIR:   C86672
- RefSeq:   NP_266535.1
- ProteinModelPortal:   Q9CII1
- SMR:   Q9CII1
- GeneID:   1113990
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L183112
- NMPDR:   fig|272623.1.peg.387
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   LLAC272623:L183112-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45225; Mature: 45093

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS51165 THUMP

Important sites: BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGAD
CEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
YAEVSNRLTKVFGIQNFSPSIKVEKSMPIIKEEVVALFKEIYSEGQTFKIATRRADHNFE
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
LDSTELNIALGDVVFDNFSYAKVQMKKPDITLRAEIRQDAAYLSFEDIKGAGGMPVGTAG
CCCCEEEEEEEEEHCCCCCEEEEEEECCCEEEEEEHHHCHHEEEHHHCCCCCCCCCCCCC
RGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGSIT
CCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCEEE
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTL
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GSMSAINEVTNTPVIRPVVTMDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPK
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHCEECCCCCCCCCC
LENCIQYEKRMDVEGMVDRAVKGIMLTTIIGENWDQTEEEEFADFL
HHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TVLYDEIMVRYGELSTKGKNRGYFINRLAKNIREVLADMPELKITAVRDRAHIELNGAD
EEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCC
YAEVSNRLTKVFGIQNFSPSIKVEKSMPIIKEEVVALFKEIYSEGQTFKIATRRADHNFE
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
LDSTELNIALGDVVFDNFSYAKVQMKKPDITLRAEIRQDAAYLSFEDIKGAGGMPVGTAG
CCCCEEEEEEEEEHCCCCCEEEEEEECCCEEEEEEHHHCHHEEEHHHCCCCCCCCCCCCC
RGHLMLSGGIDSPVAGYLALKRGVEIEAVHFASPPYTSPGALKKAKDLAAKLTVFGGSIT
CCEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCEEE
FIEVPFTEIQEEIKAKAPQAYLMTITRRFMMRVVDRVREERGGKVIINGESLGQVASQTL
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
GSMSAINEVTNTPVIRPVVTMDKNEIIEIAEKIDTFNLSILPFEDCCTVFAPPSPKTNPK
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHCEECCCCCCCCCC
LENCIQYEKRMDVEGMVDRAVKGIMLTTIIGENWDQTEEEEFADFL
HHHHHHHHHHCCHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471