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The map label for this gene is tra1077C

Identifier: 15672354

GI number: 15672354

Start: 374892

End: 375710

Strand: Direct

Name: tra1077C

Synonym: L0468

Alternate gene names: 15672354

Gene position: 374892-375710 (Clockwise)

Preceding gene: 15672353

Following gene: 15672357

Centisome position: 15.85

GC content: 34.8

Gene sequence:

>819_bases
TTGAAAGTTACAGGATTCCCTAAAGCCACTTATTATTATTGGGTTAACTGTTTTGAACGGGTAAACAAAGATGAGCTTAT
CGAAAAAGAAATGCTTAAAATACGTCAAGAACATGCCAATGCAGGTTATCGTCCAATGCGTGAATTACTTAAGCAACGTG
GCTATCACGTCAACCACAAGAAGGTACAGCGCTTAATGAAGAAGCTAGGGCTTCGTGTGACGTCTTATTGGCACAAATCA
CGTAAATATAATTCATATAAAGGAAAAGTAGGGACGGTAGCTAAAAATAAATTGCACAGACGATTCAGAACTTCCATTCC
TCATCAAAAAATCACAACAGATACGACAGAATTCAAATATTATGAAGATGGAATTCAGAAAAAGTGTTATCTCAATCCTT
ACATTGCTTTATTTAATAGCGAGGTGATTAGTTATCATATCTCTAAACAACCCTCTTATCAATCAATTGATATTGCTCTA
AATCAAGCCTTAGCTGTGACATCTGATTGTCCTTATCGACGTACTTTCCACTCAGATCAAGGATGGGGATACCAGATGAG
AGACTATGTTTCTAAATTAAAATCTCATAGAATTTTTCAGTCTATGAGCCGTAAAGGAAACTGTCATGATAATTCAGTTA
TGGAGAATTTCTTTGGGCTACTTAAACAAGAAATTTACTACGGACATATCTTCTCGTCTTTCGAAGAACTTGAACAGGTT
ATTGTAATCTGGATTAGGTACTATAATACAAAACGAATCAAACAAAAATTGAACTGGATGAGTCCAATTCAATTTCGCTT
GAATTACCAAAACAATTAA

Upstream 100 bases:

>100_bases
CTTATTTAAAAGGACTTCGGAGATTGGAGAAAAATGCTCTAATGAACAAAAATCAAGACTCATCTACAGTCTCCGTAAAA
CCTTCAAACTCAAAGAAATC

Downstream 100 bases:

>100_bases
AAAAATCCAAGTATTTCTGCTTGGATAAAAAGTCTAACTTTTGGGGTGCACATCAAAAAGCGGTTTTTATTTTTTATTTC
ATAGTTGGGAAAAGCAAAAC

Product: IS1077C transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIALFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Sequences:

>Translated_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIALFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN
>Mature_272_residues
MKVTGFPKATYYYWVNCFERVNKDELIEKEMLKIRQEHANAGYRPMRELLKQRGYHVNHKKVQRLMKKLGLRVTSYWHKS
RKYNSYKGKVGTVAKNKLHRRFRTSIPHQKITTDTTEFKYYEDGIQKKCYLNPYIALFNSEVISYHISKQPSYQSIDIAL
NQALAVTSDCPYRRTFHSDQGWGYQMRDYVSKLKSHRIFQSMSRKGNCHDNSVMENFFGLLKQEIYYGHIFSSFEELEQV
IVIWIRYYNTKRIKQKLNWMSPIQFRLNYQNN

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=268, Percent_Identity=41.4179104477612, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788405, Length=275, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787263, Length=275, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786753, Length=275, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786570, Length=275, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786490, Length=275, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32813; Mature: 32813

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]