Click here to switch to the map view.

The map label for this gene is glpF1

Identifier: 15672233

GI number: 15672233

Start: 247656

End: 248372

Strand: Direct

Name: glpF1

Synonym: L47650

Alternate gene names: 15672233

Gene position: 247656-248372 (Clockwise)

Preceding gene: 15672232

Following gene: 15672235

Centisome position: 10.47

GC content: 42.54

Gene sequence:

>717_bases
ATGACATTACAAATATTGGGAGAATTTCTGGGCACTTTCATCCTTGTATTATTAGGAAATGGGGTTGTCGCAGCAAATGT
TTTAGGTAAAACAAAATCGGAAAATGCAGGTTGGGTGACTATTGCAATTGGCTGGGGCTTAGCCGTTGCTGTCGCTGTCT
GGGTAGTTGGCTTCTTTTCACCCGCTTATCTCAATCCAGCCTTGGTCATTGGTTTTCTTGTCTTAGGGAAAATAAAGATT
GGCTTAGCATTTGCTTTTATTGTAGCCGAATTTTTAGGTGCTATGTTAGGTGCTGTCGCCGTTTGGTTACATTATTATCC
TCATTGGGAAGAAACAAAAGATTCTTCCCTAATCTTGGCAAGTTTTGCCACAGCGCCCGCTATTCGTTATTCTTGGTCCA
ATTTTTTAGGAGAAGCTTTTGATGGGGCGCTACTCATGATACTGATTATGGCAATGGGCCATTATCGTCTGGAAGGTGGT
TTTGGTCCAATTATTGTCGGTTTTCTTATTATGGCAGTTGGTTTTTCTTTAGGACCAACAACTGGCTACGCGATGAATCC
AGCACGTGACCTAGGTCCGAGAATAATGCACGCACTTTTACCAATCAAAAATAAAGGAACTTCCGGCTGGGGCTATGCTT
GGATTCCAGCAACTGGTTCAATTGTTGGTGCTGTAATTGCTGGACTACTCTATCAATGGATGTTGACGCTCCATTGA

Upstream 100 bases:

>100_bases
GGGAGCTTATACAGCGAGTGCCCTCTTAGAAGCAGGAGCAACTTTTGAAGCAATAAAAGAGCAGTTAGAAAAAATGCTGA
TAGAAAAATAAGGTAGAGCA

Downstream 100 bases:

>100_bases
TAGTATCTAAAAAATACGGATAATCCATCCGTATTTTTTTAATCATTAAAATCAAAGCGAAGTTTCATGTGATTGGAACC
AGAAATAACCATTTTACCAA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTLQILGEFLGTFILVLLGNGVVAANVLGKTKSENAGWVTIAIGWGLAVAVAVWVVGFFSPAYLNPALVIGFLVLGKIKI
GLAFAFIVAEFLGAMLGAVAVWLHYYPHWEETKDSSLILASFATAPAIRYSWSNFLGEAFDGALLMILIMAMGHYRLEGG
FGPIIVGFLIMAVGFSLGPTTGYAMNPARDLGPRIMHALLPIKNKGTSGWGYAWIPATGSIVGAVIAGLLYQWMLTLH

Sequences:

>Translated_238_residues
MTLQILGEFLGTFILVLLGNGVVAANVLGKTKSENAGWVTIAIGWGLAVAVAVWVVGFFSPAYLNPALVIGFLVLGKIKI
GLAFAFIVAEFLGAMLGAVAVWLHYYPHWEETKDSSLILASFATAPAIRYSWSNFLGEAFDGALLMILIMAMGHYRLEGG
FGPIIVGFLIMAVGFSLGPTTGYAMNPARDLGPRIMHALLPIKNKGTSGWGYAWIPATGSIVGAVIAGLLYQWMLTLH
>Mature_237_residues
TLQILGEFLGTFILVLLGNGVVAANVLGKTKSENAGWVTIAIGWGLAVAVAVWVVGFFSPAYLNPALVIGFLVLGKIKIG
LAFAFIVAEFLGAMLGAVAVWLHYYPHWEETKDSSLILASFATAPAIRYSWSNFLGEAFDGALLMILIMAMGHYRLEGGF
GPIIVGFLIMAVGFSLGPTTGYAMNPARDLGPRIMHALLPIKNKGTSGWGYAWIPATGSIVGAVIAGLLYQWMLTLH

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=256, Percent_Identity=37.109375, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=32.03125, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI4826645, Length=258, Percent_Identity=32.1705426356589, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=28.3950617283951, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1790362, Length=260, Percent_Identity=32.6923076923077, Blast_Score=104, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994009, Length=243, Percent_Identity=29.6296296296296, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=247, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=247, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=28.2258064516129, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=249, Percent_Identity=26.1044176706827, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=249, Percent_Identity=26.5060240963855, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17544068, Length=250, Percent_Identity=31.6, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=239, Percent_Identity=27.1966527196653, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=235, Percent_Identity=25.9574468085106, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=215, Percent_Identity=29.3023255813954, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=25.089605734767, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652747, Length=238, Percent_Identity=28.1512605042017, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551084, Length=238, Percent_Identity=28.1512605042017, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25376; Mature: 25244

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQILGEFLGTFILVLLGNGVVAANVLGKTKSENAGWVTIAIGWGLAVAVAVWVVGFFS
CHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCC
PAYLNPALVIGFLVLGKIKIGLAFAFIVAEFLGAMLGAVAVWLHYYPHWEETKDSSLILA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
SFATAPAIRYSWSNFLGEAFDGALLMILIMAMGHYRLEGGFGPIIVGFLIMAVGFSLGPT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCC
TGYAMNPARDLGPRIMHALLPIKNKGTSGWGYAWIPATGSIVGAVIAGLLYQWMLTLH
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLQILGEFLGTFILVLLGNGVVAANVLGKTKSENAGWVTIAIGWGLAVAVAVWVVGFFS
HHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCC
PAYLNPALVIGFLVLGKIKIGLAFAFIVAEFLGAMLGAVAVWLHYYPHWEETKDSSLILA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
SFATAPAIRYSWSNFLGEAFDGALLMILIMAMGHYRLEGGFGPIIVGFLIMAVGFSLGPT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCC
TGYAMNPARDLGPRIMHALLPIKNKGTSGWGYAWIPATGSIVGAVIAGLLYQWMLTLH
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]