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The map label for this gene is pdhC

Identifier: 15672042

GI number: 15672042

Start: 60583

End: 62181

Strand: Reverse

Name: pdhC

Synonym: L0035

Alternate gene names: 15672042

Gene position: 62181-60583 (Counterclockwise)

Preceding gene: 15672043

Following gene: 15672041

Centisome position: 2.63

GC content: 40.59

Gene sequence:

>1599_bases
ATGACTGAGATTTTTAAAATGCCTGATATTGGCGAAGGAATGCACGAAGGAGACATTGCCAACTGGTTAGTTAAAGTTGG
AGATGTTGTTAAAGAAGATGACCCAATTGCTGAAGTACAAAATGATAAACTTATGCAAGAAATTCTCTCTCCTTATTCTG
GTACCGTAACAAAACTTTTCGTTGAAGCTGGAACAACCGTAGAAGTTGATTCTCCTTTGGTTGAGTTTGATGGTGACGGT
TCAGGCTCTTCTGCAGCTGCTCCTACTCCTCAAGAAACAGCTGGTTCTGATACTGCTACAACTGATGCACCTTCTGGAGA
AGCTCAAATCTTCACGATGCCTGATATTGGTGAAGGAATGCATGAAGGAGATATCGCCAACTGGTTAGTTAAAGTTGGAG
ATGAAATTAAAGAAGATGATCCTGTTGCAGAGGTTCAAAATGATAAACTCATGCAAGAAATTCTTTCTCCATATTCTGGA
AAAGTCACTAAACTTTTCGTTGAAGCTGGAACGACTGTAGAAGTTGGGGCTCCTTTAATTGAATACAATGGAAATGGGGC
TGCTCCAGCAGCTGCTAGTCCTGCTCCAGTCGCAGAAGCACCTAAAGCAGCTAGTCCTGCAAGTCCTGCAAATGCTCCAT
TGACTAAAACAACTTCTACTGGTCACATTCTTGCGATGCCTTCTGTTCGTCATTATGCACGCAAGGCTGGTATTGATTTA
AGTCAAGTTCCTGCAACTGGTCGTCACGGTCACACAACGTTGGCCGATGTTAAAGCTTTTGAATCTGGCAGTGTCGCTCC
TATCGCTCAAACAACACCAGAAGCCGCTCCAGCACCTAAGGCTGATAAACCAGCTGCTCCTGTTGCTGAAAAAGCGCCAA
GCGTTAAAGCAGGAGCTGGTGATCGACGTGAAGCAATGAATCCAACTCGTAAAGTTGTTTCTAAAGTTATGACTGCTCAA
CATACACACATTCCACCAGTAACAAACTTTGACCAAGTTGAAGTTTCTAAACTCGTGAAACATCGTGCTGTCTTTAAAGA
AATTGCTGCAAAACAAGATATTAAATTAACCTATCTTGCTTATGTTGCTAAAGCTTTGGCTACTACTGCTCATAAATTCC
CAGATATCAATGCTTCTGTTGACTATGAAAAACAAGAAATTGTTTATCACGAACATGTCAACCTTGGAATCGCTGTAAAT
GCTCCAACTGGTTTGTACGTTCCAGTGATTCATGAAGCTGAAACTAAATCAATTCTTGAGATTGCTAAAGAAATCGCTGA
ATTAGCTACTGCTACACGAGAAGGAACACTTAAACCTCAACAAATGCAAGGTTCAACAATTACAATTTCAAATATTGGAT
CTGCTCGTGGTTCTTGGTTTACACCAATCATTAATGGTTCTGATGTCGTAATTTTAGGACTCGGTTCAATCGTTAAAGAA
CCAATTGTTAATGGTGAAGGCGAAATTGTTGTCGGACAAAACATGAAATTGTCTATGACTTATGATCACCGTTTGATTGA
TGGAATGTTGGGTCAAACATCACTTAACTACTTGAAATCTCTATTGGCTGACCCTGAGTTCATGCTTATGGAAATCTAA

Upstream 100 bases:

>100_bases
GTTAGTGGTCCTGACTCAATTTTCCCATTTGCACAAGCAGAAAATGACTGGGCAGTTAAAGCTGAAGATATTGTGAATAA
AGTTAAAGAGGTGGTTGACT

Downstream 100 bases:

>100_bases
TAGGCTGTAATAAATGGTGCTAAAGCATCTATTTATTACAGGCGTGTCTCCTAGAGGATACTCGCTAATTGTCTTATTAG
TAAAGGAACGAAAAAATATC

Product: dihydrolipoamide acetyltransferase component of PDH complex

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEAGTTVEVDSPLVEFDGDG
SGSSAAAPTPQETAGSDTATTDAPSGEAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSG
KVTKLFVEAGTTVEVGAPLIEYNGNGAAPAAASPAPVAEAPKAASPASPANAPLTKTTSTGHILAMPSVRHYARKAGIDL
SQVPATGRHGHTTLADVKAFESGSVAPIAQTTPEAAPAPKADKPAAPVAEKAPSVKAGAGDRREAMNPTRKVVSKVMTAQ
HTHIPPVTNFDQVEVSKLVKHRAVFKEIAAKQDIKLTYLAYVAKALATTAHKFPDINASVDYEKQEIVYHEHVNLGIAVN
APTGLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKE
PIVNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI

Sequences:

>Translated_532_residues
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEAGTTVEVDSPLVEFDGDG
SGSSAAAPTPQETAGSDTATTDAPSGEAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSG
KVTKLFVEAGTTVEVGAPLIEYNGNGAAPAAASPAPVAEAPKAASPASPANAPLTKTTSTGHILAMPSVRHYARKAGIDL
SQVPATGRHGHTTLADVKAFESGSVAPIAQTTPEAAPAPKADKPAAPVAEKAPSVKAGAGDRREAMNPTRKVVSKVMTAQ
HTHIPPVTNFDQVEVSKLVKHRAVFKEIAAKQDIKLTYLAYVAKALATTAHKFPDINASVDYEKQEIVYHEHVNLGIAVN
APTGLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKE
PIVNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
>Mature_531_residues
TEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLFVEAGTTVEVDSPLVEFDGDGS
GSSAAAPTPQETAGSDTATTDAPSGEAQIFTMPDIGEGMHEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGK
VTKLFVEAGTTVEVGAPLIEYNGNGAAPAAASPAPVAEAPKAASPASPANAPLTKTTSTGHILAMPSVRHYARKAGIDLS
QVPATGRHGHTTLADVKAFESGSVAPIAQTTPEAAPAPKADKPAAPVAEKAPSVKAGAGDRREAMNPTRKVVSKVMTAQH
THIPPVTNFDQVEVSKLVKHRAVFKEIAAKQDIKLTYLAYVAKALATTAHKFPDINASVDYEKQEIVYHEHVNLGIAVNA
PTGLYVPVIHEAETKSILEIAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKEP
IVNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=31.685393258427, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI31711992, Length=591, Percent_Identity=26.0575296108291, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI203098816, Length=498, Percent_Identity=25.9036144578313, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=25.4424778761062, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=30, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786305, Length=537, Percent_Identity=30.9124767225326, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=29.1764705882353, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=33.4096109839817, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=422, Percent_Identity=30.5687203791469, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=26.8722466960352, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=27.0700636942675, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=30.9133489461358, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=27.5938189845475, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=31.4685314685315, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24645909, Length=246, Percent_Identity=32.520325203252, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=27.8761061946903, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=27.8761061946903, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56317; Mature: 56186

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
VEAGTTVEVDSPLVEFDGDGSGSSAAAPTPQETAGSDTATTDAPSGEAQIFTMPDIGEGM
EECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
HEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVTKLFVEAGTTVEVGAPLI
CCCHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCEE
EYNGNGAAPAAASPAPVAEAPKAASPASPANAPLTKTTSTGHILAMPSVRHYARKAGIDL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHHCCCH
SQVPATGRHGHTTLADVKAFESGSVAPIAQTTPEAAPAPKADKPAAPVAEKAPSVKAGAG
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
DRREAMNPTRKVVSKVMTAQHTHIPPVTNFDQVEVSKLVKHRAVFKEIAAKQDIKLTYLA
CHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YVAKALATTAHKFPDINASVDYEKQEIVYHEHVNLGIAVNAPTGLYVPVIHEAETKSILE
HHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCEEEEEEECCCCEEEEEEECCHHHHHHH
IAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKE
HHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCCCEECEEECCCCEEEEECHHHHHH
PIVNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
HCCCCCCCEEECCCEEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TEIFKMPDIGEGMHEGDIANWLVKVGDVVKEDDPIAEVQNDKLMQEILSPYSGTVTKLF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
VEAGTTVEVDSPLVEFDGDGSGSSAAAPTPQETAGSDTATTDAPSGEAQIFTMPDIGEGM
EECCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
HEGDIANWLVKVGDEIKEDDPVAEVQNDKLMQEILSPYSGKVTKLFVEAGTTVEVGAPLI
CCCHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCEE
EYNGNGAAPAAASPAPVAEAPKAASPASPANAPLTKTTSTGHILAMPSVRHYARKAGIDL
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHHHCCCH
SQVPATGRHGHTTLADVKAFESGSVAPIAQTTPEAAPAPKADKPAAPVAEKAPSVKAGAG
HHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
DRREAMNPTRKVVSKVMTAQHTHIPPVTNFDQVEVSKLVKHRAVFKEIAAKQDIKLTYLA
CHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YVAKALATTAHKFPDINASVDYEKQEIVYHEHVNLGIAVNAPTGLYVPVIHEAETKSILE
HHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECCEEEEEEECCCCEEEEEEECCHHHHHHH
IAKEIAELATATREGTLKPQQMQGSTITISNIGSARGSWFTPIINGSDVVILGLGSIVKE
HHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCCCCEECEEECCCCEEEEECHHHHHH
PIVNGEGEIVVGQNMKLSMTYDHRLIDGMLGQTSLNYLKSLLADPEFMLMEI
HCCCCCCCEEECCCEEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]