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The map label for this gene is 15668855

Identifier: 15668855

GI number: 15668855

Start: 599724

End: 600740

Strand: Direct

Name: 15668855

Synonym: MJ0674

Alternate gene names: NA

Gene position: 599724-600740 (Clockwise)

Preceding gene: 15668854

Following gene: 15668866

Centisome position: 36.02

GC content: 29.2

Gene sequence:

>1017_bases
ATGAAACTTGGTAGATATTTAGCGGTTTCAAAAGATTTAGCCCCAGCAAAGTTTATAATAGCTAAGTGTATAGAAGTTGA
AGAATTTAAGGGATTAGAACTAAATGAATTATGGGAAATCCATAACAAGGTCTTGAAAAAAGTAGATTTTGATAATTTTG
ATTTTAATGATTTAGAGTATGTAAAACCAAACCTATTGGACTTAAAAGTAGAGATTGCCAAAAATATCTTTAAAAATTGC
CATTTTTGTGAGCATAGATGTTATGTAAATAGAGAAACTGAAAGAGGTTTCTGTAGGATAAAAGAGAGCTATTACTCAAC
AGAATTTCTACATCTCGGTGAAGAAAGAGTTTTAGTTCCATCACATACAATCTTCTTCTGTGGCTGTAATTTTAAATGTG
TCTTCTGCCAGAACTGGGATATATCTCAGGTTTATTTTGATAAAACAATTCCTAACCACTGTATTCCATATAATCCAAAA
GAAATGGCTAAAATTATTAAACATAAAAGAGATTATTCAAAAAACGTTAATTTTGTTGGTGGAGACCCTACACCACATTT
ATTGAGTATTTTAAAAACTCTAAGCTATTTAGATAAAAACATCCCGGTAGTTTGGAATTCAAATATGTATCTAACTGTTG
AAGGAATGCATTTATTAAAAGGAGTTGTTGATGTCTATTTAACTGACTTTAAGTTTGGAAATAATGAATGTGGAGAAAGA
TTATCAAAAGTTAAAAACTATTTTGATATAATTAAGAGGAATCACTTGCTTATAAAAGATGAGGAAGTTATAATAAGGCA
TTTAGTAATGCCAAACCACTTAGATTGTTGCACAGAGAAAATATTTGACTTCATCTCAAAAAATTTAGATAACGCTGTAG
TTAATGTTATGTTTCAGTATCGCCCAGAATATAAAGCTAAGGAATATCCTGATATAAATAGAAGATTAACTTATGAAGAG
ATAGAGAAGGCATTGGAGTTGGCAGAGAAATATAATTTAGATTTAATCTATGATTAA

Upstream 100 bases:

>100_bases
CAATATTAATTAAGGAATAAATTTTTCAAGCTTAATTTTTAATTTTTTATACTTCATATAATTTATCTATTTAAATTAAT
TACTCAACTGATGTGAGATT

Downstream 100 bases:

>100_bases
ATTTTTAATTATTCATTTTTCAACTTTTCCCTCTTAATCATTTTAAATTTCTTCCCTCCACACTCACAAACTAAATCCTC
AACTTTCTTAACCTCTTCTC

Product: pyruvate formate lyase-activating enzyme PflX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEYVKPNLLDLKVEIAKNIFKNC
HFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVPSHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPK
EMAKIIKHKRDYSKNVNFVGGDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGER
LSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQYRPEYKAKEYPDINRRLTYEE
IEKALELAEKYNLDLIYD

Sequences:

>Translated_338_residues
MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEYVKPNLLDLKVEIAKNIFKNC
HFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVPSHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPK
EMAKIIKHKRDYSKNVNFVGGDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGER
LSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQYRPEYKAKEYPDINRRLTYEE
IEKALELAEKYNLDLIYD
>Mature_338_residues
MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEYVKPNLLDLKVEIAKNIFKNC
HFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVPSHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPK
EMAKIIKHKRDYSKNVNFVGGDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGER
LSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQYRPEYKAKEYPDINRRLTYEE
IEKALELAEKYNLDLIYD

Specific function: Unknown

COG id: COG1313

COG function: function code R; Uncharacterized Fe-S protein PflX, homolog of pyruvate formate lyase activating proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y674_METJA (Q58087)

Other databases:

- EMBL:   L77117
- PIR:   B64384
- RefSeq:   NP_247658.1
- ProteinModelPortal:   Q58087
- GeneID:   1451540
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0674
- NMPDR:   fig|243232.1.peg.695
- TIGR:   MJ0674
- HOGENOM:   HBG312575
- OMA:   YIDVYLP
- ProtClustDB:   CLSK2749907
- BioCyc:   MJAN243232:MJ_0674-MONOMER
- InterPro:   IPR016431
- InterPro:   IPR007197
- PIRSF:   PIRSF004869

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 40021; Mature: 40021

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEY
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
VKPNLLDLKVEIAKNIFKNCHFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVP
CCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHEEHHHHHCHHHEECCCCEEEEC
SHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPKEMAKIIKHKRDYSKNVNFVG
CCEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC
GDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGER
CCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHH
LSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQY
HHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEE
RPEYKAKEYPDINRRLTYEEIEKALELAEKYNLDLIYD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEY
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
VKPNLLDLKVEIAKNIFKNCHFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVP
CCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHEEHHHHHCHHHEECCCCEEEEC
SHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPKEMAKIIKHKRDYSKNVNFVG
CCEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEC
GDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGER
CCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHHHH
LSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQY
HHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEE
RPEYKAKEYPDINRRLTYEEIEKALELAEKYNLDLIYD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087