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The map label for this gene is purA

Identifier: 15668741

GI number: 15668741

Start: 495585

End: 496622

Strand: Direct

Name: purA

Synonym: MJ0561

Alternate gene names: 15668741

Gene position: 495585-496622 (Clockwise)

Preceding gene: 15668739

Following gene: 15668748

Centisome position: 29.77

GC content: 36.9

Gene sequence:

>1038_bases
GTGAAAAAGGTGGTTTTATTGACTTGCACCATTATTGTTGGAGGACAATGGGGAGATGAAGGAAAAGGAAAGATAATAAG
CTATATTTGTGATAAAGACAAGCCATCAATTATTGCAAGAGGAGGAGTAGGACCAAACGCTGGGCATACTGTAAATATTG
GTGGAAAAAGTTATGGAATTAGAATGATACCTACAGGATTCCCATACAAAGAGGCAAAGTTGGCTATAGGGGCTGGTGTT
TTGGTAGATCCAGAAGTTTTGTTAAAAGAGGTAGAGATGCTTAAAGACTTTAATGTTAAAGAGAGATTGATTGTAGATTA
TAGATGTGGAATTATTGAAGAGAAGCATAAAATTATGGACAGAAAAGATGAGCACTTGGCTAAAGAGATTGGAACTACTG
GAAGCGGCTGTGGCCCTGCAAACGTTGATAGAGTGTTGAGGATTTTAAAGCAGGCAAAGGATATTGAAGAGCTTAAAGAA
TTTTTAGGAGATGTCTCTGAAGAGGTTAATAATGCATTGGATAGAGGAGAAAATGTTTTAATTGAAGGAACACAGGGAAC
TTTACTCTCTTTATATTATGGAACCTATCCTTATGTAACATCCAAGGATACAACAGCTTCATCATTTGCCGCTGATGTTG
GAATCGGCCCTACAAAAGTTGATGAGGTTATAGTTGTCTTTAAAACCTTCCCTACAAGAGTTGGTGCTGGGCCATTTCCA
ACTGAAATGTCATTAGAAGAGGCAGAGAGCTTGGGAATTGTTGAGTATGGAACAGTTACTGGAAGAAGGAGAAGAGTTGG
CTATTTTGACTTTGAATTAGCAAGGAAAGCTTGTAGGTTGAATGGAGCAACACAGATTGCTTTAACTGGATTAGATAAAT
ACGATAAAGAGTGTTATGGAGTAACTGAATATAATAAGCTAAGTGAAAAAGCTAAAGAATTTATAAACAAGATTGAAGAA
GTTACTGGAGTTCCAGTAACTATAATCTCCACAGGTCCAGAAATGCACCAAACAATTGATTTAAGAAATGAGCTATAA

Upstream 100 bases:

>100_bases
TATAAAAAACTTATAATAACTTACTTGCCATACATTGGAAATTCTTAATATTAATTAACTTTAAATATTACAATTACAAA
AAAATATAAGGTTTTGAACA

Downstream 100 bases:

>100_bases
AATTTAACTAAATTTCATATTATATTAGATAATCTTTTTTAAGGTTAATATAAACCATGCCTAACTAAATCGGAATTACT
GAAACGCTATCTTTTATAAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGIRMIPTGFPYKEAKLAIGAGV
LVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMDRKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKE
FLGDVSEEVNNALDRGENVLIEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFP
TEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYGVTEYNKLSEKAKEFINKIEE
VTGVPVTIISTGPEMHQTIDLRNEL

Sequences:

>Translated_345_residues
MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGIRMIPTGFPYKEAKLAIGAGV
LVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMDRKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKE
FLGDVSEEVNNALDRGENVLIEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFP
TEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYGVTEYNKLSEKAKEFINKIEE
VTGVPVTIISTGPEMHQTIDLRNEL
>Mature_345_residues
MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGIRMIPTGFPYKEAKLAIGAGV
LVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMDRKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKE
FLGDVSEEVNNALDRGENVLIEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFP
TEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYGVTEYNKLSEKAKEFINKIEE
VTGVPVTIISTGPEMHQTIDLRNEL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=340, Percent_Identity=32.3529411764706, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI34577063, Length=344, Percent_Identity=31.3953488372093, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI40316944, Length=305, Percent_Identity=30.8196721311475, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=35.5294117647059, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI25146555, Length=425, Percent_Identity=30.3529411764706, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25146553, Length=425, Percent_Identity=30.3529411764706, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=29.5508274231678, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI21356033, Length=369, Percent_Identity=30.3523035230352, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_METJA (Q57981)

Other databases:

- EMBL:   L77117
- PIR:   A64370
- RefSeq:   NP_247540.1
- ProteinModelPortal:   Q57981
- SMR:   Q57981
- GeneID:   1451426
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0561
- NMPDR:   fig|243232.1.peg.577
- TIGR:   MJ0561
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK04293
- BioCyc:   MJAN243232:MJ_0561-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 37821; Mature: 37821

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2; PS00014 ER_TARGET

Important sites: ACT_SITE 19-19 ACT_SITE 49-49 BINDING 133-133 BINDING 147-147 BINDING 185-185 BINDING 200-200 BINDING 262-262 BINDING 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGI
CCEEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCE
RMIPTGFPYKEAKLAIGAGVLVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMD
EECCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHC
RKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKEFLGDVSEEVNNALDRGENVL
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFP
EECCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
TEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYG
CCCCHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCC
VTEYNKLSEKAKEFINKIEEVTGVPVTIISTGPEMHQTIDLRNEL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGI
CCEEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEECCCCCCE
RMIPTGFPYKEAKLAIGAGVLVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMD
EECCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHC
RKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKEFLGDVSEEVNNALDRGENVL
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFP
EECCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
TEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYG
CCCCHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCC
VTEYNKLSEKAKEFINKIEEVTGVPVTIISTGPEMHQTIDLRNEL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087