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The map label for this gene is hisH [H]

Identifier: 15668683

GI number: 15668683

Start: 447120

End: 447716

Strand: Reverse

Name: hisH [H]

Synonym: MJ0506

Alternate gene names: 15668683

Gene position: 447716-447120 (Counterclockwise)

Preceding gene: 15668684

Following gene: 15668678

Centisome position: 26.89

GC content: 31.16

Gene sequence:

>597_bases
ATGAAAATGATTGGAATAATTGATTACAACGCAGGGAATTTGAGAAGTATTCAAAAGGCAGTTGAACTCTATGATAAGGT
AATAATAACAAACAACAGTGAGGAGTTATTGGCTTGTGATAAGATAATTCTACCAGGTGTAGGAAATTTTGGTAGTGCAA
TGGAAAATTTAGCTCCATTAAAAGAGACAATATACAAAATTGTTGATGATAGAGTTCCATTCTTAGGAATATGTTTAGGA
ATGCAGATTTTATTTGAAGAGAGCGAAGAGAAAAGAGGAATCAAAGGTTTAGGGATAATAAAAGGCAATGTAATCAAGTT
TAAGGATGTTGAAAAACTTCCACATATGGGCTGGAATAGTGTAAAAATAGTTAAAGATTGCCCACTGTTTGAAGGAATAA
AAAACAATAGTTACTTTTACTTTGTTCATTCATATCATGTAAATCCAGATGAAGATTGTATAGTTGGAAAAACTGAATAT
GGAAGAGAGTTTCCAAGCGTTATAAACAAAGATAATGTCTTTGCCACCCAATTCCACCCAGAAAAAAGTGGAAAAATTGG
TTTAAAGATTATAGAAAATTTTGTTGAGTTGTTATAA

Upstream 100 bases:

>100_bases
GGTCGTTATTACTGGTTTAAAGAGTGAGGAAGATGCCAAAAGAGCTCTAAAGAAGATTTTAGATACAATAAAAGAAGTTC
AAGAACTCTAAATTTTAGGG

Downstream 100 bases:

>100_bases
TTAATTTTTTGACGTTTCATTTAAGAAATAATCTAAAAACTCTAATCCACTGCCATAATTAACTGCCTTTGGATGAATTG
AGAGAAAAAACTTTCTATTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKMIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPLKETIYKIVDDRVPFLGICLG
MQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNSVKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEY
GREFPSVINKDNVFATQFHPEKSGKIGLKIIENFVELL

Sequences:

>Translated_198_residues
MKMIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPLKETIYKIVDDRVPFLGICLG
MQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNSVKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEY
GREFPSVINKDNVFATQFHPEKSGKIGLKIIENFVELL
>Mature_198_residues
MKMIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPLKETIYKIVDDRVPFLGICLG
MQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNSVKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEY
GREFPSVINKDNVFATQFHPEKSGKIGLKIIENFVELL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=194, Percent_Identity=40.2061855670103, Blast_Score=144, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=37.2641509433962, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22360; Mature: 22360

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPL
CCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCHHEEECEEEECCCCCHHHHHHHHHHH
KETIYKIVDDRVPFLGICLGMQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEEECCEEEECCHHHCCCCCCCC
VKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEYGREFPSVINKDNVFATQFHP
EEEEECCCCCCCCCCCCEEEEEEEEECCCCCCEEECCHHHCHHHHHHHCCCCEEEEEECC
EKSGKIGLKIIENFVELL
CCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKMIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPL
CCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCHHEEECEEEECCCCCHHHHHHHHHHH
KETIYKIVDDRVPFLGICLGMQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEEECCEEEECCHHHCCCCCCCC
VKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEYGREFPSVINKDNVFATQFHP
EEEEECCCCCCCCCCCCEEEEEEEEECCCCCCEEECCHHHCHHHHHHHCCCCEEEEEECC
EKSGKIGLKIIENFVELL
CCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]