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The map label for this gene is leuC

Identifier: 15668676

GI number: 15668676

Start: 439827

End: 441101

Strand: Reverse

Name: leuC

Synonym: MJ0499

Alternate gene names: 15668676

Gene position: 441101-439827 (Counterclockwise)

Preceding gene: 15668677

Following gene: 15668675

Centisome position: 26.49

GC content: 38.35

Gene sequence:

>1275_bases
ATGGGAATGACAATTGTAGAGAAGATATTAGCAAAGGCGTCTGGAAAGAAGGAAGTTAGTCCTGGAGATATAGTGATGGC
AAACATTGATGTAGCAATGGTTCATGATATTACAGGGCCTTTAACAGTCAATACATTAAAGGAGTATGGAATTGAAAAAG
TTTGGAATCCAGAAAAGATAGTTATTTTATTTGACCACCAAGTTCCTGCTGATAGTATAAAAGCGGCTGAAAACCATATA
TTAATGAGAAAGTTCGTAAAAGAACAGGGTATTAAATACTTCTACGATATTAGAGAGGGAGTTTGTCACCAAGTTTTACC
AGAGAAAGGACATGTAGCTCCAGGAGAGGTAGTTGTTGGAGCTGATTCACACACATGCACACATGGAGCTTTTGGTGCTT
TTGCTACCGGTATAGGTTCAACTGACATGGCTCACGTATTTGCAACAGGTAAATTGTGGTTTAAAGTTCCAGAAACAATA
TACTTCAACATTACTGGAGATTTACAACCTTACGTTACTTCAAAGGATGTTATTCTAAGCATTATAGGAGAAGTTGGTGT
TGATGGGGCTACATATAAAGCATGCCAGTTTGGTGGAGAAACCGTTAAAAAGATGTCCATAGCATCAAGAATGACAATGA
CAAACATGGCTATTGAGATGGGGGGAAAAACAGGAATTATAGAGCCAGATGAGAAAACCATCCAATATGTAAAAGAGGCT
ATGAAGAAACATGGAACTGAGAGACCATTTGAGGTAATAAAAGGAGATGAAGATGCTGAATTTGCAGAGGTTTATGAAAT
TGAGGCAGATAAAATAGAGCCAGTATTTGCATGCCCACACAATGTAGATAATGTTAAACAGGCGAGAGAAGTGGCTGGAA
AGCCTATAGACCAGGTGTTTATTGGTTCATGTACGAACGGAAGATTGGAAGATTTAAGAATGGCTATTAAGATTATTGAG
AAGCATGGTGGAATTGCTGATGATGTTAGGGTTGTTGTAACTCCAGCTTCAAGGGAAGAGTATCTAAAAGCATTAAAAGA
GGGAATAATTGAGAAATTCTTAAAGTATGGATGTGTTGTTACAAATCCTTCATGCTCTGCTTGTATGGGTTCATTGTATG
GTGTTTTAGGTCCTGGAGAGGTCTGTGTCTCAACCTCAAACAGAAACTTCAGAGGAAGGCAGGGTTCATTAGAAGCAGAG
ATTTATTTAGCATCACCAATAACTGCTGCTGCATGTGCTGTTAAAGGAGAACTTGTTGACCCAAGGGATTTATAA

Upstream 100 bases:

>100_bases
CTAAAGGAAGGCGTTCAAAGTTTAATAATAAGTTTATTAATTTTGAAAGGCACTATATATCTACAGTTATTCTTATAAAG
ACTAATTAAATGGTGAGATT

Downstream 100 bases:

>100_bases
TTTTTCCATAATTCTTTTTAAAACATTTAAAAAAGGCAGGCACTAATAGTATTCTATTTTAAAGCTTTAAACATTTGGGG
TTTGCATAAATAACAATCTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MGMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFDHQVPADSIKAAENHI
LMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETI
YFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEA
MKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVFIGSCTNGRLEDLRMAIKIIE
KHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAE
IYLASPITAAACAVKGELVDPRDL

Sequences:

>Translated_424_residues
MGMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFDHQVPADSIKAAENHI
LMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETI
YFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEA
MKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVFIGSCTNGRLEDLRMAIKIIE
KHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAE
IYLASPITAAACAVKGELVDPRDL
>Mature_423_residues
GMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFDHQVPADSIKAAENHIL
MRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIY
FNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEAM
KKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVFIGSCTNGRLEDLRMAIKIIEK
HGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEI
YLASPITAAACAVKGELVDPRDL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=415, Percent_Identity=25.7831325301205, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI8659555, Length=460, Percent_Identity=22.3913043478261, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI41352693, Length=387, Percent_Identity=23.5142118863049, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1786259, Length=443, Percent_Identity=36.7945823927765, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI2367097, Length=436, Percent_Identity=27.0642201834862, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081781, Length=433, Percent_Identity=22.4018475750577, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787531, Length=390, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=415, Percent_Identity=26.5060240963855, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=411, Percent_Identity=26.5206812652068, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=294, Percent_Identity=26.530612244898, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=22.962962962963, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321429, Length=448, Percent_Identity=35.4910714285714, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320440, Length=449, Percent_Identity=31.1804008908686, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6323335, Length=419, Percent_Identity=28.1622911694511, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=360, Percent_Identity=27.7777777777778, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28571643, Length=416, Percent_Identity=28.3653846153846, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI281365315, Length=414, Percent_Identity=26.5700483091787, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17864292, Length=414, Percent_Identity=26.5700483091787, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=26.5853658536585, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137564, Length=468, Percent_Identity=23.5042735042735, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645686, Length=259, Percent_Identity=27.7992277992278, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_METJA (P81291)

Other databases:

- EMBL:   L77117
- PIR:   C64362
- RefSeq:   NP_247475.1
- ProteinModelPortal:   P81291
- GeneID:   1451361
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0499
- NMPDR:   fig|243232.1.peg.512
- TIGR:   MJ0499
- HOGENOM:   HBG330745
- OMA:   PASRTEY
- ProtClustDB:   PRK00402
- BioCyc:   MetaCyc:MONOMER-13647
- BioCyc:   MJAN243232:MJ_0499-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31

Molecular weight: Translated: 46108; Mature: 45976

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKI
CCHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCEEEHHHHHCCHHHCCCCCEE
VILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEE
ADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILS
CCCCCCCCCCHHHHHHCCCCCCCEEEEEECEEEEEECCEEEEEEECCCCCCCCCHHHHHH
IIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEA
HHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHH
MKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVF
HHHCCCCCCHHHHCCCCCCCHHHHEEECCCCCCEEEECCCCHHHHHHHHHHHCCCHHHEE
IGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVV
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
TNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVD
ECCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCC
PRDL
CCCC
>Mature Secondary Structure 
GMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKI
CHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCCCEEEHHHHHCCHHHCCCCCEE
VILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEE
ADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILS
CCCCCCCCCCHHHHHHCCCCCCCEEEEEECEEEEEECCEEEEEEECCCCCCCCCHHHHHH
IIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEA
HHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHH
MKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVF
HHHCCCCCCHHHHCCCCCCCHHHHEEECCCCCCEEEECCCCHHHHHHHHHHHCCCHHHEE
IGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVV
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
TNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVD
ECCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCC
PRDL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087