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Identifier: 15645735

GI number: 15645735

Start: 1187005

End: 1187943

Strand: Reverse

Name: Not Available

Synonym: HP1121

Alternate gene names: 15645735

Gene position: 1187943-1187005 (Counterclockwise)

Preceding gene: 15645736

Following gene: 15645734

Centisome position: 71.23

GC content: 36.21

Gene sequence:

>939_bases
ATGGATTTTTGCTCTGGCATTGGTGGAGGCCGTTTGGGCTTGGAGCAATGCCATTTAAAATGCGTAGGGCATGCAGAAAT
CAATCATGAAGCCCTTAGGACTTATGAATTATTTTTTAAAGATACCCATAATTTTGGGGATTTGATGCGAATCAACCCTA
ATGATTTACCCGATTTTGATGCACTCATTAGCGGGTTTCCTTGTCAAGCTTTTTCTATCAATGGCAAAAGGAAGGGGCTT
GAAGATGAAAGAGGGACGATTATTTACGGGCTTATTCGCATTTTAAAAGTTAAACAGCCTGAATGTTTCTTGCTTGAAAA
TGTTAAGGGCTTGATCAATCATAATAAAAAGGCAACTTTTAATATTATTATCAAAGCCCTACAAGAAGTGGGTTATACAA
CTTATTATAAAATTTTAAACAGCGCTGATTTTCAATTAGCCCAAAATAGAGAACGCCTTTATATCGTAGGGTTTAGGAAG
GATTTAAAACACCCATTTAATTTCCCTTTAGGTTTAGCCAATGATTATTATTTCAAGGATTTTTTAGACGCTGATAATGA
ATGTTATTTGGATGTGAGTAACGCTGCATTTCAAAGATACTTGCACAACCGATACAACCATAACCGGGTTTCTTTAGAGG
ATCTCTTAACTTTAGAAAACGCTGTTTTAGACACAAGACAATCTGATTTAAGGTTGTATTCTAATGTTTTTCCTACTTTA
AGGACTTCTCGGCATGGCCTGTTTTATACCCAAAAAGGCAAAATCAAAAGATTAAACGCTATTGAAAGCTTGCTTTTGCA
AGGATTTCCTAGGGATTTGATCGCTAAGATTAAAGATAATCCTAACTTTAAAGCAAGCCATTTGCTATCCCAAGCGGGGA
ATGCGATGAGCGTGAATGTGATTGCTGCAATCGCTAAACAAATGTTAAAGGCGATTTAA

Upstream 100 bases:

>100_bases
TTTTAGTTTCTCTGTGTTTTAAAATGAGTTTGTTGCTTTCTTAAATAGGGCATGTTTTTAGTCGTGTGTTAGGAATGATT
GATTGGGGATTTTAACTTTT

Downstream 100 bases:

>100_bases
TAAGGGAGCTTTAAGGGGAGAATGATTTCAAAATACCCCCTATCCCCTTAAGAAATTGGGTTTTTACAATAAAAAAATGG
CATAGTATTTGCTTGAATAC

Product: cytosine specific DNA methyltransferase (BSP6IM)

Products: NA

Alternate protein names: M.NgoBI; Cytosine-specific methyltransferase NgoBI; M.NgoI [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDFCSGIGGGRLGLEQCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGLIRILKVKQPECFLLENVKGLINHNKKATFNIIIKALQEVGYTTYYKILNSADFQLAQNRERLYIVGFRK
DLKHPFNFPLGLANDYYFKDFLDADNECYLDVSNAAFQRYLHNRYNHNRVSLEDLLTLENAVLDTRQSDLRLYSNVFPTL
RTSRHGLFYTQKGKIKRLNAIESLLLQGFPRDLIAKIKDNPNFKASHLLSQAGNAMSVNVIAAIAKQMLKAI

Sequences:

>Translated_312_residues
MDFCSGIGGGRLGLEQCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGLIRILKVKQPECFLLENVKGLINHNKKATFNIIIKALQEVGYTTYYKILNSADFQLAQNRERLYIVGFRK
DLKHPFNFPLGLANDYYFKDFLDADNECYLDVSNAAFQRYLHNRYNHNRVSLEDLLTLENAVLDTRQSDLRLYSNVFPTL
RTSRHGLFYTQKGKIKRLNAIESLLLQGFPRDLIAKIKDNPNFKASHLLSQAGNAMSVNVIAAIAKQMLKAI
>Mature_312_residues
MDFCSGIGGGRLGLEQCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFDALISGFPCQAFSINGKRKGL
EDERGTIIYGLIRILKVKQPECFLLENVKGLINHNKKATFNIIIKALQEVGYTTYYKILNSADFQLAQNRERLYIVGFRK
DLKHPFNFPLGLANDYYFKDFLDADNECYLDVSNAAFQRYLHNRYNHNRVSLEDLLTLENAVLDTRQSDLRLYSNVFPTL
RTSRHGLFYTQKGKIKRLNAIESLLLQGFPRDLIAKIKDNPNFKASHLLSQAGNAMSVNVIAAIAKQMLKAI

Specific function: This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=34.6153846153846, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 35656; Mature: 35656

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFCSGIGGGRLGLEQCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
ALISGFPCQAFSINGKRKGLEDERGTIIYGLIRILKVKQPECFLLENVKGLINHNKKATF
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
NIIIKALQEVGYTTYYKILNSADFQLAQNRERLYIVGFRKDLKHPFNFPLGLANDYYFKD
HHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCEEEEEEEHHHCCCCCCCCCCCCCCHHHHH
FLDADNECYLDVSNAAFQRYLHNRYNHNRVSLEDLLTLENAVLDTRQSDLRLYSNVFPTL
HHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RTSRHGLFYTQKGKIKRLNAIESLLLQGFPRDLIAKIKDNPNFKASHLLSQAGNAMSVNV
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEHHH
IAAIAKQMLKAI
HHHHHHHHHHHC
>Mature Secondary Structure
MDFCSGIGGGRLGLEQCHLKCVGHAEINHEALRTYELFFKDTHNFGDLMRINPNDLPDFD
CCCCCCCCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
ALISGFPCQAFSINGKRKGLEDERGTIIYGLIRILKVKQPECFLLENVKGLINHNKKATF
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
NIIIKALQEVGYTTYYKILNSADFQLAQNRERLYIVGFRKDLKHPFNFPLGLANDYYFKD
HHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCEEEEEEEHHHCCCCCCCCCCCCCCHHHHH
FLDADNECYLDVSNAAFQRYLHNRYNHNRVSLEDLLTLENAVLDTRQSDLRLYSNVFPTL
HHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RTSRHGLFYTQKGKIKRLNAIESLLLQGFPRDLIAKIKDNPNFKASHLLSQAGNAMSVNV
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCEEHHH
IAAIAKQMLKAI
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9628358 [H]