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The map label for this gene is moaA

Identifier: 15607116

GI number: 15607116

Start: 1534728

End: 1535690

Strand: Direct

Name: moaA

Synonym: aq_2183

Alternate gene names: 15607116

Gene position: 1534728-1535690 (Clockwise)

Preceding gene: 15607115

Following gene: 15607119

Centisome position: 98.93

GC content: 40.91

Gene sequence:

>963_bases
ATGCTTCTGGACAAACTAAGTAGACCGCTGAAAGTCCTCAGGATTTCTCTAACGGATAGATGCAACCTCAGGTGCAACTT
CTGCATGCCCCCGGGAAAGGAGTATAACTTCCTTCCTAAAAGACAACTACTTACCCCCGAAGAAATAGAGGAGTACGTCA
AGATATTTGCCAAACTCGGCGTTGAAAAAGTTCGCCTAACAGGCGGTGAACCTCTCCTGAGGGAAGATTTAGAGGAGATA
ATTCAAAGAATTTCAAAAGTAGAAGGAATTAAAGATATAGCTCTAACGACTAACGGCGTATTTTTAAAAGAAAGGTTAAA
AGCTTTAAAAGAAGCGGGATTGAAGAGGATTACCGTAAGCGTACACTCACTGAATCCCGAAAAGAACCAAAAACTTGTTA
ATAGAAGTGTTAACTTAGGTGAAGTCTTTGAAGTAATAATAAGAGCAAAAGAGCTTGGCTTTAAAGTGAAGGTGAACTCC
GTAATAATAAAGGGTTTTAACGACGACGAGATTCTGGATCTTGCAAGGTTCTTTAAAAACCTGGGTGTAACGCTGAGGTT
TATAGAGTACATGGACGTGGGAACGGTTAACGACTGGGATTTTTCAAAGGTTGTAAGTGCGGACGAAATTCTGAACTTAA
TGAAGAAAGAGTTTACATTTTATCCTCTTCCCAAAAGACCCGAAGATACGTCAATGGATTTCATTTACGAGGATGGGAAT
AAGTTCGGGATAATAGCCTCGGTGACGAAACCCTTCTGCAGGGGTTGTAACCGCATAAGACTTTCTGCAGACGGTAAGCT
CTACACATGCCTCTTTTCGGATAAAGGACATGACTTGAGAAACGCCGTTGATAAAGAAAACTTTATTAAAGAAGTCTGGA
AAGACAGAAAAGACAGGTACTCAGAACTCAGGCGGCAGATGAAGAGAGAGAGAAAAGTAGAAATGTTCAAAGTCGGCGGT
TAA

Upstream 100 bases:

>100_bases
TGCAGGTACGCCGTTGACGAGACGAAAAAACGCGTCCCCATATGGAAGAAGGAAATCTTTAAGGAAGGAAAAGGAGAGTG
GGTCCTAGGGGAAAAGAAAA

Downstream 100 bases:

>100_bases
TGTTTTAAGAGTCCGTAAAGTATGAGCTTTTTAATGGTTTCAGGATCCTTAATGATCTTTCCCTTTTTACCTCTGAGGAA
CCATTGGAAGGCCTTTGCCT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLLDKLSRPLKVLRISLTDRCNLRCNFCMPPGKEYNFLPKRQLLTPEEIEEYVKIFAKLGVEKVRLTGGEPLLREDLEEI
IQRISKVEGIKDIALTTNGVFLKERLKALKEAGLKRITVSVHSLNPEKNQKLVNRSVNLGEVFEVIIRAKELGFKVKVNS
VIIKGFNDDEILDLARFFKNLGVTLRFIEYMDVGTVNDWDFSKVVSADEILNLMKKEFTFYPLPKRPEDTSMDFIYEDGN
KFGIIASVTKPFCRGCNRIRLSADGKLYTCLFSDKGHDLRNAVDKENFIKEVWKDRKDRYSELRRQMKRERKVEMFKVGG

Sequences:

>Translated_320_residues
MLLDKLSRPLKVLRISLTDRCNLRCNFCMPPGKEYNFLPKRQLLTPEEIEEYVKIFAKLGVEKVRLTGGEPLLREDLEEI
IQRISKVEGIKDIALTTNGVFLKERLKALKEAGLKRITVSVHSLNPEKNQKLVNRSVNLGEVFEVIIRAKELGFKVKVNS
VIIKGFNDDEILDLARFFKNLGVTLRFIEYMDVGTVNDWDFSKVVSADEILNLMKKEFTFYPLPKRPEDTSMDFIYEDGN
KFGIIASVTKPFCRGCNRIRLSADGKLYTCLFSDKGHDLRNAVDKENFIKEVWKDRKDRYSELRRQMKRERKVEMFKVGG
>Mature_320_residues
MLLDKLSRPLKVLRISLTDRCNLRCNFCMPPGKEYNFLPKRQLLTPEEIEEYVKIFAKLGVEKVRLTGGEPLLREDLEEI
IQRISKVEGIKDIALTTNGVFLKERLKALKEAGLKRITVSVHSLNPEKNQKLVNRSVNLGEVFEVIIRAKELGFKVKVNS
VIIKGFNDDEILDLARFFKNLGVTLRFIEYMDVGTVNDWDFSKVVSADEILNLMKKEFTFYPLPKRPEDTSMDFIYEDGN
KFGIIASVTKPFCRGCNRIRLSADGKLYTCLFSDKGHDLRNAVDKENFIKEVWKDRKDRYSELRRQMKRERKVEMFKVGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=283, Percent_Identity=40.6360424028269, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI22027536, Length=283, Percent_Identity=40.6360424028269, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1786999, Length=288, Percent_Identity=34.375, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=286, Percent_Identity=39.1608391608392, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI32566331, Length=320, Percent_Identity=35.9375, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI32566329, Length=286, Percent_Identity=37.7622377622378, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28574490, Length=296, Percent_Identity=39.1891891891892, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574488, Length=296, Percent_Identity=39.1891891891892, Blast_Score=202, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_AQUAE (O67929)

Other databases:

- EMBL:   AE000657
- PIR:   E70487
- RefSeq:   NP_214498.1
- ProteinModelPortal:   O67929
- SMR:   O67929
- GeneID:   1193918
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2183
- NMPDR:   fig|224324.1.peg.1513
- HOGENOM:   HBG529885
- OMA:   ILQARIF
- PhylomeDB:   O67929
- BioCyc:   AAEO224324:AQ_2183-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37125; Mature: 37125

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 14-14 BINDING 65-65 BINDING 69-69 BINDING 96-96 BINDING 120-120 BINDING 157-157 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDKLSRPLKVLRISLTDRCNLRCNFCMPPGKEYNFLPKRQLLTPEEIEEYVKIFAKLG
CCHHHHCCCHHHEEEECCCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHC
VEKVRLTGGEPLLREDLEEIIQRISKVEGIKDIALTTNGVFLKERLKALKEAGLKRITVS
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHCCCEEEEEE
VHSLNPEKNQKLVNRSVNLGEVFEVIIRAKELGFKVKVNSVIIKGFNDDEILDLARFFKN
EECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHH
LGVTLRFIEYMDVGTVNDWDFSKVVSADEILNLMKKEFTFYPLPKRPEDTSMDFIYEDGN
CCCCEEEHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCEECCCCCCCCCCCCEEEEECCC
KFGIIASVTKPFCRGCNRIRLSADGKLYTCLFSDKGHDLRNAVDKENFIKEVWKDRKDRY
EEEEEEEHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SELRRQMKRERKVEMFKVGG
HHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MLLDKLSRPLKVLRISLTDRCNLRCNFCMPPGKEYNFLPKRQLLTPEEIEEYVKIFAKLG
CCHHHHCCCHHHEEEECCCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHC
VEKVRLTGGEPLLREDLEEIIQRISKVEGIKDIALTTNGVFLKERLKALKEAGLKRITVS
CCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHHHCCCEEEEEE
VHSLNPEKNQKLVNRSVNLGEVFEVIIRAKELGFKVKVNSVIIKGFNDDEILDLARFFKN
EECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHH
LGVTLRFIEYMDVGTVNDWDFSKVVSADEILNLMKKEFTFYPLPKRPEDTSMDFIYEDGN
CCCCEEEHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHCEECCCCCCCCCCCCEEEEECCC
KFGIIASVTKPFCRGCNRIRLSADGKLYTCLFSDKGHDLRNAVDKENFIKEVWKDRKDRY
EEEEEEEHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SELRRQMKRERKVEMFKVGG
HHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320