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The map label for this gene is potB [H]

Identifier: 15602128

GI number: 15602128

Start: 295900

End: 296754

Strand: Reverse

Name: potB [H]

Synonym: PM0263

Alternate gene names: 15602128

Gene position: 296754-295900 (Counterclockwise)

Preceding gene: 15602129

Following gene: 15602127

Centisome position: 13.15

GC content: 39.06

Gene sequence:

>855_bases
ATGAAGAATAACGCCTTTCAGAAATCAACCATTGGTGTCATCTTCGGGTGGTTGATTTTCTTTGTTCTTGCGCCAAACTT
ACTTGTGCTGATCGTGAGTTTTTTAACTCGCGATAGCAGTAATTTCTATGCGCTTCCTTTTACATTGGAAAACTATACAC
GCTTATTTGAGCCGCTATATGCGCAAGTGGTATGGAATTCCTTATATATGTCGGGTTTAGCCACCTTAGTGTGTCTGATT
ATCGGCTATCCTTTTGCCTTTTTATTAACCAAAATTAACCCGAAATACCGTCCATTTTTACTGTTTTTAGTGGTGTTACC
TTTCTGGACCAACTCCCTAATTCGTATTTATGGTATGAAAATTTTCTTAGGGGTCAAAGGGATTTTAAATAGCACACTCA
TGTCCATTGGCTTAATTGATACGCCTATCCGTATTTTGAATACAGAAGTCGCGGTCATCATTGGTTTAGTGTATTTATTG
CTGCCATTTATGATTTTGCCACTTTATGCCTCTATTGAGAAATTGGATTATCGCTTACTGGAAGCGGCAAAAGATCTGGG
TGCTAATGCATTCCAACGCTTTATCAAAGTGATTATTCCGCTCACGATGCCGGGTATTATCGCAGGTTGTTTATTAGTGC
TTTTACCAGCCATGGGGATGTTCTATGTCGCTGATTTATTAGGCGGTGCCAAAGTCCTCTTAGTGGGTAACGTGATTAAG
AGTGAATTCTTGATCTCACGTAACTGGCCGTTTGGTTCTGCAATCAGCATTGGATTAACTATCCTGATGGCATTACTGAT
CTTTGTTTACTACAAAGCCAGCAAATTAATGAATAAGAAAATGGAGTTGGAATAA

Upstream 100 bases:

>100_bases
GTTAGCGAATTCTTTAACGAAGACGATCCAAATATCGATCACTCTTTAGGTCAAAAAGTGGCATTAACATGGCACGAAGG
CTGGGAGGTAGTGTTGAGCG

Downstream 100 bases:

>100_bases
TGGGCCGTTTACTACGCAATATTTTTATGTTTGTGGTCTATGCTTACTTGTATATTCCAATTGCAATCTTAGTCACAAAC
TCGTTTAACGATGATCGTTA

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKNNAFQKSTIGVIFGWLIFFVLAPNLLVLIVSFLTRDSSNFYALPFTLENYTRLFEPLYAQVVWNSLYMSGLATLVCLI
IGYPFAFLLTKINPKYRPFLLFLVVLPFWTNSLIRIYGMKIFLGVKGILNSTLMSIGLIDTPIRILNTEVAVIIGLVYLL
LPFMILPLYASIEKLDYRLLEAAKDLGANAFQRFIKVIIPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNVIK
SEFLISRNWPFGSAISIGLTILMALLIFVYYKASKLMNKKMELE

Sequences:

>Translated_284_residues
MKNNAFQKSTIGVIFGWLIFFVLAPNLLVLIVSFLTRDSSNFYALPFTLENYTRLFEPLYAQVVWNSLYMSGLATLVCLI
IGYPFAFLLTKINPKYRPFLLFLVVLPFWTNSLIRIYGMKIFLGVKGILNSTLMSIGLIDTPIRILNTEVAVIIGLVYLL
LPFMILPLYASIEKLDYRLLEAAKDLGANAFQRFIKVIIPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNVIK
SEFLISRNWPFGSAISIGLTILMALLIFVYYKASKLMNKKMELE
>Mature_284_residues
MKNNAFQKSTIGVIFGWLIFFVLAPNLLVLIVSFLTRDSSNFYALPFTLENYTRLFEPLYAQVVWNSLYMSGLATLVCLI
IGYPFAFLLTKINPKYRPFLLFLVVLPFWTNSLIRIYGMKIFLGVKGILNSTLMSIGLIDTPIRILNTEVAVIIGLVYLL
LPFMILPLYASIEKLDYRLLEAAKDLGANAFQRFIKVIIPLTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNVIK
SEFLISRNWPFGSAISIGLTILMALLIFVYYKASKLMNKKMELE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=274, Percent_Identity=64.963503649635, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=43.37899543379, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787713, Length=252, Percent_Identity=30.5555555555556, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31905; Mature: 31905

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]