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The map label for this gene is tgt

Identifier: 15602094

GI number: 15602094

Start: 255944

End: 257101

Strand: Reverse

Name: tgt

Synonym: PM0229

Alternate gene names: 15602094

Gene position: 257101-255944 (Counterclockwise)

Preceding gene: 15602095

Following gene: 15602093

Centisome position: 11.39

GC content: 42.83

Gene sequence:

>1158_bases
ATGAAATACGAACTTGATAAAACCGATGGCAACGCACGACGTGGTCGTCTTGTGTTTGAACGTCCACAAGGTACCTTTAC
GGTAGAAACCCCTGCTTTTATGCCAGTAGGCACCTACGGTACGGTAAAAGGCATGACGCCTGAAGAAGTCCGTGCTACGG
GGGCAGAGATTCTATTAGGTAACACATTCCATTTATGGTTACGTCCCGGACAAGAGGTGATGCGTAAGCACGGTGATTTA
CATGACTTTATGCAATGGCATCGTCCAATTCTCACAGACTCGGGCGGTTTCCAAGTGTTCAGTTTAGGCAAATTGCGTAA
AATTACGGAAGAAGGGGTAAAATTCCAAAACCCAATTAATGGGGAACGTATTTTCTTGTCGCCAGAAAAATCAATGGAAA
TTCAATATGATCTAGGGTCAGATATCGTGATGATTTTCGACGAATGTACCCCTTACCCTGCCACCTTTGATTATGCAAAA
AAATCCATGGAAATGTCGTTACGTTGGGCAAAACGTAGCCGTGATCGTTTTGATGAGCTTGGTAATAAAAATGCATTATT
TGGTATCATTCAAGGTGGCGTTTACGAAGAATTACGTAAAGTGTCGGTTGAAGGATTAGTCAACATTGGCTTTGATGGTT
ATGCGGTCGGCGGTTTAGCCGTCGGTGAACCGAAAGAAGATATGCACCGTATTTTAGAATACGTCTGTCCACAAATTCCC
GCTGATAAACCACGTTATTTAATGGGAGTGGGTAAACCAGAAGATTTAGTCGAAGGGGTGCGCCGTGGTATCGATATGTT
CGATTGTGTGATGCCAACCCGTAATGCACGTAATGGGCATTTATTTGTCAGCGATGGTATTGTAAAAATCCGTAATGCCA
AATACCGTGAGGATACCAGCCCATTAGATCCAGAATGTGACTGTTATACCTGTAAACATTACACCAAGGCGTACTTGTAT
CACTTAGATAAGTGCGGTGAAATTTTAGGCGCAAGATTAAACACAATTCATAACTTACGCTATTATCAGCGCTTAATGGC
GCAAATTCGCCAAGCTATCGAAGAAGATCGCTTTGAGGCATTTGTACAGGAATTCTATGCCAAAATGGGCAAACCTGTCC
CACCTATGCAGTCTGAAATAAAATCAGATGAAGGCTAA

Upstream 100 bases:

>100_bases
AAAGAATATTGAACAACCTTATCTCAAAAGCCTGTTTCTTGATACAGGCTTTTTTATTCACCACATGCTTCGAACTGTTT
ATTCGTTGAAAGGAAAGGAA

Downstream 100 bases:

>100_bases
GCCTGAATCCAATTGGATTAAAACATGGGGTAATGAGATAAATTTACCCCATTTTTTATGCTTAGTTGGCTTTCTTTTGT
TCCTTCCCACGTTGATTTTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKYELDKTDGNARRGRLVFERPQGTFTVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEVMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVYEELRKVSVEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYVCPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSDGIVKIRNAKYREDTSPLDPECDCYTCKHYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFEAFVQEFYAKMGKPVPPMQSEIKSDEG

Sequences:

>Translated_385_residues
MKYELDKTDGNARRGRLVFERPQGTFTVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEVMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVYEELRKVSVEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYVCPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSDGIVKIRNAKYREDTSPLDPECDCYTCKHYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFEAFVQEFYAKMGKPVPPMQSEIKSDEG
>Mature_385_residues
MKYELDKTDGNARRGRLVFERPQGTFTVETPAFMPVGTYGTVKGMTPEEVRATGAEILLGNTFHLWLRPGQEVMRKHGDL
HDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPINGERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
KSMEMSLRWAKRSRDRFDELGNKNALFGIIQGGVYEELRKVSVEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYVCPQIP
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSDGIVKIRNAKYREDTSPLDPECDCYTCKHYTKAYLY
HLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFEAFVQEFYAKMGKPVPPMQSEIKSDEG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=374, Percent_Identity=43.3155080213904, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1786607, Length=379, Percent_Identity=81.5303430079156, Blast_Score=660, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=386, Percent_Identity=42.7461139896373, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=169, Percent_Identity=33.7278106508876, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=44.4141689373297, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21355311, Length=269, Percent_Identity=27.5092936802974, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_PASMU (P57831)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245166.1
- ProteinModelPortal:   P57831
- SMR:   P57831
- PRIDE:   P57831
- GeneID:   1243576
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0229
- NMPDR:   fig|272843.1.peg.229
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   PMUL272843:PM0229-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 44014; Mature: 44014

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELDKTDGNARRGRLVFERPQGTFTVETPAFMPVGTYGTVKGMTPEEVRATGAEILLG
CCEECCCCCCCCCCCCEEEECCCCCEEECCCCCEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEVMRKHGDLHDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPIN
CEEEEEECCCHHHHHHCCCHHHHHHHCCCCEECCCCEEEEECHHHHHHHHHCCEECCCCC
GERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKKSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNKNALFGIIQGGVYEELRKVSVEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYVCPQIP
CCCCCEEEEECCHHHHHHHHHHHHHHEECCCCCEEECCEECCCCHHHHHHHHHHHCCCCC
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSDGIVKIRNAKYREDTS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
PLDPECDCYTCKHYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFEA
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVQEFYAKMGKPVPPMQSEIKSDEG
HHHHHHHHHCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MKYELDKTDGNARRGRLVFERPQGTFTVETPAFMPVGTYGTVKGMTPEEVRATGAEILLG
CCEECCCCCCCCCCCCEEEECCCCCEEECCCCCEECCCCCCCCCCCHHHHHHCCCEEEEC
NTFHLWLRPGQEVMRKHGDLHDFMQWHRPILTDSGGFQVFSLGKLRKITEEGVKFQNPIN
CEEEEEECCCHHHHHHCCCHHHHHHHCCCCEECCCCEEEEECHHHHHHHHHCCEECCCCC
GERIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAKKSMEMSLRWAKRSRDRFDEL
CCEEEECCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GNKNALFGIIQGGVYEELRKVSVEGLVNIGFDGYAVGGLAVGEPKEDMHRILEYVCPQIP
CCCCCEEEEECCHHHHHHHHHHHHHHEECCCCCEEECCEECCCCHHHHHHHHHHHCCCCC
ADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVSDGIVKIRNAKYREDTS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
PLDPECDCYTCKHYTKAYLYHLDKCGEILGARLNTIHNLRYYQRLMAQIRQAIEEDRFEA
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVQEFYAKMGKPVPPMQSEIKSDEG
HHHHHHHHHCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100