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The map label for this gene is smpB

Identifier: 15602082

GI number: 15602082

Start: 240798

End: 241280

Strand: Reverse

Name: smpB

Synonym: PM0217

Alternate gene names: 15602082

Gene position: 241280-240798 (Counterclockwise)

Preceding gene: 15602084

Following gene: 15602078

Centisome position: 10.69

GC content: 41.2

Gene sequence:

>483_bases
ATGACAAAGAAAAAAGTAAAAGTTGGCACAAATACGATAGCCTTAAATAAACGTGCACGACACGATTATTTTATTGAAGA
CGAAATGGAAGCGGGTCTGGAATTGCAAGGCTGGGAAGTGAAGTCGATGCGTGCTGGAAAAGCCAACATTAGTGACAGTT
ATATTATCTTTAAACAAGGCGAAGCTTATTTATTTGGTGCAACAATTCAGCCACTTAATGTCGCTTCAACGCATATTGTG
TGTGATCCCACACGGACACGCAAGCTGTTACTCAAGCAAAAAGAACTGGCATCATTATTTGGGAAAGCCAATCGTGATGG
CTATACTATCGTTGCTCTCTCACTCTATTGGAAAGGCGCTTGGGCTAAACTGAAAATTGGTCTTGCCAAAGGGAAAAAAC
AGCACGACAAACGTGAAGATATCAAAGAACGCGAGTGGAAAGTCACGAAAGATCGTATTATGAAAAATGCACGCCTTGGC
TAA

Upstream 100 bases:

>100_bases
AAATATGTTATAGACAACAAGCTTGATTGCAAGCAATTTTGCTATCTCAAGCAACTTTCTGTAAAATCAAGCAAATTAGA
ATTTTATGACGCGGATTATT

Downstream 100 bases:

>100_bases
AAAAAAAAAGCCTTGTGAGCATCATCACAAGGCTTTTTTAAACTAGCGAGATTATTCTACACCGACCATCACAGAAGTCG
CTTTAATAATAGCGTAAGCG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTKKKVKVGTNTIALNKRARHDYFIEDEMEAGLELQGWEVKSMRAGKANISDSYIIFKQGEAYLFGATIQPLNVASTHIV
CDPTRTRKLLLKQKELASLFGKANRDGYTIVALSLYWKGAWAKLKIGLAKGKKQHDKREDIKEREWKVTKDRIMKNARLG

Sequences:

>Translated_160_residues
MTKKKVKVGTNTIALNKRARHDYFIEDEMEAGLELQGWEVKSMRAGKANISDSYIIFKQGEAYLFGATIQPLNVASTHIV
CDPTRTRKLLLKQKELASLFGKANRDGYTIVALSLYWKGAWAKLKIGLAKGKKQHDKREDIKEREWKVTKDRIMKNARLG
>Mature_159_residues
TKKKVKVGTNTIALNKRARHDYFIEDEMEAGLELQGWEVKSMRAGKANISDSYIIFKQGEAYLFGATIQPLNVASTHIVC
DPTRTRKLLLKQKELASLFGKANRDGYTIVALSLYWKGAWAKLKIGLAKGKKQHDKREDIKEREWKVTKDRIMKNARLG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=158, Percent_Identity=76.5822784810127, Blast_Score=257, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PASMU (P57827)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245154.1
- ProteinModelPortal:   P57827
- SMR:   P57827
- GeneID:   1243564
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0217
- NMPDR:   fig|272843.1.peg.217
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   PMUL272843:PM0217-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18268; Mature: 18136

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKVKVGTNTIALNKRARHDYFIEDEMEAGLELQGWEVKSMRAGKANISDSYIIFKQG
CCCCEEEECCCEEEECCCCCCCCEECCHHHCCCEECCEEEECCCCCCCCCCCCEEEEECC
EAYLFGATIQPLNVASTHIVCDPTRTRKLLLKQKELASLFGKANRDGYTIVALSLYWKGA
CEEEEECEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC
WAKLKIGLAKGKKQHDKREDIKEREWKVTKDRIMKNARLG
EEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TKKKVKVGTNTIALNKRARHDYFIEDEMEAGLELQGWEVKSMRAGKANISDSYIIFKQG
CCCEEEECCCEEEECCCCCCCCEECCHHHCCCEECCEEEECCCCCCCCCCCCEEEEECC
EAYLFGATIQPLNVASTHIVCDPTRTRKLLLKQKELASLFGKANRDGYTIVALSLYWKGA
CEEEEECEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC
WAKLKIGLAKGKKQHDKREDIKEREWKVTKDRIMKNARLG
EEEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100