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The map label for this gene is dnaQ

Identifier: 15601971

GI number: 15601971

Start: 132010

End: 132771

Strand: Reverse

Name: dnaQ

Synonym: PM0106

Alternate gene names: 15601971

Gene position: 132771-132010 (Counterclockwise)

Preceding gene: 15601973

Following gene: 15601970

Centisome position: 5.88

GC content: 43.31

Gene sequence:

>762_bases
ATGACCCCAGTGAATCAACCGACTCGCCAAATTGTACTGGATACCGAAACAACGGGGATGAACCAATTTGGTGCACATTA
TGAAGGGCATTGTATTATTGAAATTGGTGCGGTTGAGATGATCAATCGTCGCCTCACGGGCAATAATTTCCATATTTATA
TCAAACCGAATCGCCCCGTCGATCCCGATGCGATCAAAGTACACGGTATCACCGATGAAATGTTGGCTGATAAACCCATG
TTTAATGAAGTCGCCCAGCAATTTATTGATTATATTCAAGGGGCGGAATTGCTGATCCATAATGCGCCCTTCGACGTGGG
CTTTATGGATTATGAGTTTAAAAAACTGAATTTAAACATCAATACTGATGCGATTTGTATGGTGACTGATACCCTACAAA
TGGCACGCCAAATGTACCCCGGTAAACGTAATAGCTTAGATGCCCTTTGCGATCGTTTAGGCATTGATAACAGCAAACGA
ACCCTACACGGCGCGTTACTGGATGCGGAGATCTTAGCGGATGTGTACCTCACGATGACAGGGGGACAAACCAGTTTATT
TGATGAAAATGAACCAGAAATTGCTGTTGTCGCCGTCCAAGAACAAATCCAAAGTGCGGTGGCTTTTTCACAAGACTTAA
AACGCCTGCAACCCAATGCCGATGAACTACAAGCCCATCTCGATTACCTCCTCTTACTTAATAAAAAGAGCAAAGGCAAT
TGCTTATGGGAAAAGCGTTTAGCGGAATTGAAAACACATTAA

Upstream 100 bases:

>100_bases
CCATCCGTAAAAATTTCAATTTGTTTTTGCATAATCTTTGTTACAATTCCTCATTAAGCCAAGATCATAACCGAAATTAA
CTGAAAGAGATAGAAAGAAA

Downstream 100 bases:

>100_bases
TAAATTCGTTCATTGATTGAGCAGCTGAGGGCAAATATGAAAAAAAAAGTTGACGAAAAGCGGCTTGATCATTAATATAC
GCCTCACCTGTGCGGAGTGG

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTPVNQPTRQIVLDTETTGMNQFGAHYEGHCIIEIGAVEMINRRLTGNNFHIYIKPNRPVDPDAIKVHGITDEMLADKPM
FNEVAQQFIDYIQGAELLIHNAPFDVGFMDYEFKKLNLNINTDAICMVTDTLQMARQMYPGKRNSLDALCDRLGIDNSKR
TLHGALLDAEILADVYLTMTGGQTSLFDENEPEIAVVAVQEQIQSAVAFSQDLKRLQPNADELQAHLDYLLLLNKKSKGN
CLWEKRLAELKTH

Sequences:

>Translated_253_residues
MTPVNQPTRQIVLDTETTGMNQFGAHYEGHCIIEIGAVEMINRRLTGNNFHIYIKPNRPVDPDAIKVHGITDEMLADKPM
FNEVAQQFIDYIQGAELLIHNAPFDVGFMDYEFKKLNLNINTDAICMVTDTLQMARQMYPGKRNSLDALCDRLGIDNSKR
TLHGALLDAEILADVYLTMTGGQTSLFDENEPEIAVVAVQEQIQSAVAFSQDLKRLQPNADELQAHLDYLLLLNKKSKGN
CLWEKRLAELKTH
>Mature_252_residues
TPVNQPTRQIVLDTETTGMNQFGAHYEGHCIIEIGAVEMINRRLTGNNFHIYIKPNRPVDPDAIKVHGITDEMLADKPMF
NEVAQQFIDYIQGAELLIHNAPFDVGFMDYEFKKLNLNINTDAICMVTDTLQMARQMYPGKRNSLDALCDRLGIDNSKRT
LHGALLDAEILADVYLTMTGGQTSLFDENEPEIAVVAVQEQIQSAVAFSQDLKRLQPNADELQAHLDYLLLLNKKSKGNC
LWEKRLAELKTH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=237, Percent_Identity=63.7130801687764, Blast_Score=304, Evalue=3e-84,
Organism=Escherichia coli, GI1788149, Length=172, Percent_Identity=29.0697674418605, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO3E_PASMU (Q9CPE0)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245043.1
- ProteinModelPortal:   Q9CPE0
- SMR:   Q9CPE0
- GeneID:   1243453
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0106
- NMPDR:   fig|272843.1.peg.106
- HOGENOM:   HBG728612
- OMA:   HGITNEF
- ProtClustDB:   PRK05711
- BioCyc:   PMUL272843:PM0106-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005622
- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR00573
- TIGRFAMs:   TIGR01406

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: =2.7.7.7

Molecular weight: Translated: 28560; Mature: 28429

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 163-163 BINDING 14-14 BINDING 16-16 BINDING 63-63 BINDING 68-68 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVNQPTRQIVLDTETTGMNQFGAHYEGHCIIEIGAVEMINRRLTGNNFHIYIKPNRPV
CCCCCCCCCEEEEECCCCCCHHCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCC
DPDAIKVHGITDEMLADKPMFNEVAQQFIDYIQGAELLIHNAPFDVGFMDYEFKKLNLNI
CCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEECEEEEEEEEEE
NTDAICMVTDTLQMARQMYPGKRNSLDALCDRLGIDNSKRTLHGALLDAEILADVYLTMT
CCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEE
GGQTSLFDENEPEIAVVAVQEQIQSAVAFSQDLKRLQPNADELQAHLDYLLLLNKKSKGN
CCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCC
CLWEKRLAELKTH
HHHHHHHHHHCCC
>Mature Secondary Structure 
TPVNQPTRQIVLDTETTGMNQFGAHYEGHCIIEIGAVEMINRRLTGNNFHIYIKPNRPV
CCCCCCCCEEEEECCCCCCHHCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCC
DPDAIKVHGITDEMLADKPMFNEVAQQFIDYIQGAELLIHNAPFDVGFMDYEFKKLNLNI
CCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEECEEEEEEEEEE
NTDAICMVTDTLQMARQMYPGKRNSLDALCDRLGIDNSKRTLHGALLDAEILADVYLTMT
CCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEE
GGQTSLFDENEPEIAVVAVQEQIQSAVAFSQDLKRLQPNADELQAHLDYLLLLNKKSKGN
CCCEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCC
CLWEKRLAELKTH
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100