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The map label for this gene is lepA [H]

Identifier: 15601928

GI number: 15601928

Start: 89342

End: 91138

Strand: Reverse

Name: lepA [H]

Synonym: PM0063

Alternate gene names: 15601928

Gene position: 91138-89342 (Counterclockwise)

Preceding gene: 15601929

Following gene: 15601927

Centisome position: 4.04

GC content: 42.9

Gene sequence:

>1797_bases
ATGAAGAATATACGTAACTTTTCTATTATTGCACACATTGACCACGGTAAATCGACACTCTCTGACCGCCTTATTCAAAC
TTGCGGTGGCTTATCTGATCGTGAAATGGAAGCCCAAGTGTTGGATTCCATGGATCTTGAACGTGAACGTGGGATTACGA
TCAAAGCACAAAGTGTGACCTTAAATTACAAAGCGAAAGATGGCGAAACCTATCAATTAAATTTCATCGATACGCCAGGT
CACGTTGACTTCTCTTATGAAGTATCGCGTTCTTTAGCCGCTTGTGAAGGCGCATTATTAGTGGTGGATGCGGGACAAGG
TGTCGAGGCACAAACTTTGGCTAACTGCTATACCGCAATTGAAATGAATTTAGAAGTGGTGCCGATTTTAAACAAAATCG
ACTTGCCCGCGGCAGATCCTGAACGCGTTGCAGAAGAAATTGAAGACATTGTCGGTATTGACGCGATGGAAGCGGTGCGC
TGTTCAGCAAAAACCGGTGTGGGTATTGAAGATGTGTTGGAAGAAATTGTGCATAAAATCCCTGCACCCGAAGGGGATCC
GAATGCACCATTACAAGCCTTGATTATCGACTCGTGGTTTGATAACTACTTAGGCGTAGTATCTTTAGTGCGCATTAAAA
ACGGCACATTACGCAAAGGCGATAAAATCAAAGTGATGTCTACAGGGCAATCTTACAATGTGGATCGTCTTGGTATTTTC
ACCCCAAAACAAGTCGATACCACCATTTTAAATTGTGGTGAAGTGGGTTGGGTGGTGTGCGCCATTAAAGATATTTTAGG
GGCACCCGTGGGTGATACGCTTACTTCGCACAACAATCCAGCTTCTTCTGTCCTGCCGGGTTTTAAGAAAGTTAAGCCAC
AGGTGTATGCCGGTTTATTCCCAATTAGCTCTGATGATTATGAAGCATTCCGTGATGCGCTCGGTAAACTTAGTCTAAAC
GATGCGTCATTATTCTATGAACCAGAAAACTCCACCGCACTTGGTTTCGGTTTCCGTTGTGGTTTCTTAGGACTTCTCCA
CATGGAGATTATTCAAGAGCGTTTAGAGCGCGAATACGATCTTGATCTGATTACCACAGCACCGACAGTAGTGTATGAAG
TGGAAAAAACCGACGGTGAAGTGATTTATGTGGATAGCCCATCAAAATTACCGCCACTCAACAACATTACGGAGATTCGT
GAACCGATTGCAGAATGTAACATGCTGTTACCACAAACCTACTTAGGTAACGTCATTACGCTCTGTGTAGAAAAACGCGG
TGTACAAACCAATATGGTTTACCATGGTAACCAAGTGGCATTGACCTATGAAATCCCAATGGGCGAAGTGGTACTGGATT
TCTTCGACCGCTTAAAATCAACTTCTCGTGGTTATGCTTCCTTAGATTATGGTTTCAAACGTTTCCAAGCCGCCGATATG
GTACGTGTCGACATTATGATCAATGGTGAGCGTGTCGATGCGTTAGCGTTAATCGTGCATAAAGATAATGCACCTTATCG
TGGTCGTGAATTAGTGGAAAAAATGCGTGAGCTCATTCCACGTCAACAATTTGATATTGCGATTCAAGCGGCGATTGGTA
ACCACATTATTGCCCGTTCTACTGTCAAACAATTACGTAAAAACGTATTAGCAAAATGTTATGGTGGTGACGTTAGCCGT
AAGAAAAAACTCTTACAGAAACAAAAAGAAGGTAAAAAACGCATGAAGTCTTTGGGTAACGTCGAAGTACCACAAGAAGC
CTTCTTAGCGATTTTACATGTCGGAAAAGACAAATAA

Upstream 100 bases:

>100_bases
TTCAGTGCGCGTTTACGCTATTGCTAGCGAATTCATCAATATTCAGGTAAAATCTGGCAGTTTAACTTTATCCAATTTTT
ATACATAAATTGATTTTCAT

Downstream 100 bases:

>100_bases
GGAACATCAGGAAATACTATGTCTAACTTCTTCCTCATTCTATTGTTAGTAGCAGGATTCGGTGTCTGGAAAGTATTAGA
TTATTTTGCCTTACCTAACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLNYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMNLEVVPILNKIDLPAADPERVAEEIEDIVGIDAMEAVR
CSAKTGVGIEDVLEEIVHKIPAPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQSYNVDRLGIF
TPKQVDTTILNCGEVGWVVCAIKDILGAPVGDTLTSHNNPASSVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLN
DASLFYEPENSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVEKTDGEVIYVDSPSKLPPLNNITEIR
EPIAECNMLLPQTYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMGEVVLDFFDRLKSTSRGYASLDYGFKRFQAADM
VRVDIMINGERVDALALIVHKDNAPYRGRELVEKMRELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKSLGNVEVPQEAFLAILHVGKDK

Sequences:

>Translated_598_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLNYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMNLEVVPILNKIDLPAADPERVAEEIEDIVGIDAMEAVR
CSAKTGVGIEDVLEEIVHKIPAPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQSYNVDRLGIF
TPKQVDTTILNCGEVGWVVCAIKDILGAPVGDTLTSHNNPASSVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLN
DASLFYEPENSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVEKTDGEVIYVDSPSKLPPLNNITEIR
EPIAECNMLLPQTYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMGEVVLDFFDRLKSTSRGYASLDYGFKRFQAADM
VRVDIMINGERVDALALIVHKDNAPYRGRELVEKMRELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKSLGNVEVPQEAFLAILHVGKDK
>Mature_598_residues
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLNYKAKDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMNLEVVPILNKIDLPAADPERVAEEIEDIVGIDAMEAVR
CSAKTGVGIEDVLEEIVHKIPAPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQSYNVDRLGIF
TPKQVDTTILNCGEVGWVVCAIKDILGAPVGDTLTSHNNPASSVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLN
DASLFYEPENSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVEKTDGEVIYVDSPSKLPPLNNITEIR
EPIAECNMLLPQTYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMGEVVLDFFDRLKSTSRGYASLDYGFKRFQAADM
VRVDIMINGERVDALALIVHKDNAPYRGRELVEKMRELIPRQQFDIAIQAAIGNHIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKSLGNVEVPQEAFLAILHVGKDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=47.6973684210526, Blast_Score=601, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=46.6165413533835, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=43.75, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI25306283, Length=175, Percent_Identity=40.5714285714286, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.7631578947368, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.7631578947368, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI18390331, Length=182, Percent_Identity=34.6153846153846, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=44.5454545454545, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=44.5454545454545, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=44.5454545454545, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=32.6359832635983, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=32.6359832635983, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=37.593984962406, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=37.593984962406, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4503471, Length=170, Percent_Identity=30, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4503475, Length=171, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=89.6147403685092, Blast_Score=1078, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=480, Percent_Identity=29.375, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789738, Length=153, Percent_Identity=35.9477124183007, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789559, Length=228, Percent_Identity=30.7017543859649, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.4918032786885, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=159, Percent_Identity=35.8490566037736, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=36.4705882352941, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=36.986301369863, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.8620689655172, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=237, Percent_Identity=28.2700421940928, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=244, Percent_Identity=27.0491803278689, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=46.089850249584, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6323098, Length=292, Percent_Identity=32.1917808219178, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=42.3611111111111, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=42.3611111111111, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.8888888888889, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=241, Percent_Identity=26.9709543568465, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325337, Length=284, Percent_Identity=24.2957746478873, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=284, Percent_Identity=24.2957746478873, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.0948419301165, Blast_Score=522, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=43.4782608695652, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=36.0902255639098, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572034, Length=247, Percent_Identity=29.1497975708502, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281363316, Length=238, Percent_Identity=27.7310924369748, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864358, Length=238, Percent_Identity=27.7310924369748, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19921738, Length=240, Percent_Identity=27.9166666666667, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66212; Mature: 66212

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYKAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHE
EMNLEVVPILNKIDLPAADPERVAEEIEDIVGIDAMEAVRCSAKTGVGIEDVLEEIVHKI
EECEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHC
PAPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQSYNVDRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDTTILNCGEVGWVVCAIKDILGAPVGDTLTSHNNPASSVLPGFKKVKPQVYAGLF
CCCHHCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCCHHHCCCHHHEEEE
PISSDDYEAFRDALGKLSLNDASLFYEPENSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVEKTDGEVIYVDSPSKLPPLNNITEIREPIAECNMLLPQTYLGNVIT
CEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCVEKRGVQTNMVYHGNQVALTYEIPMGEVVLDFFDRLKSTSRGYASLDYGFKRFQAADM
HHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCE
VRVDIMINGERVDALALIVHKDNAPYRGRELVEKMRELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEECCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKSLGNVEVPQEAFLAILHVGKDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LNYKAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHE
EMNLEVVPILNKIDLPAADPERVAEEIEDIVGIDAMEAVRCSAKTGVGIEDVLEEIVHKI
EECEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHC
PAPEGDPNAPLQALIIDSWFDNYLGVVSLVRIKNGTLRKGDKIKVMSTGQSYNVDRLGIF
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDTTILNCGEVGWVVCAIKDILGAPVGDTLTSHNNPASSVLPGFKKVKPQVYAGLF
CCCHHCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCCHHHCCCHHHEEEE
PISSDDYEAFRDALGKLSLNDASLFYEPENSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVEKTDGEVIYVDSPSKLPPLNNITEIREPIAECNMLLPQTYLGNVIT
CEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCVEKRGVQTNMVYHGNQVALTYEIPMGEVVLDFFDRLKSTSRGYASLDYGFKRFQAADM
HHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCE
VRVDIMINGERVDALALIVHKDNAPYRGRELVEKMRELIPRQQFDIAIQAAIGNHIIARS
EEEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEECCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKSLGNVEVPQEAFLAILHVGKDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA