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The map label for this gene is gdhA [H]

Identifier: 15601908

GI number: 15601908

Start: 45666

End: 47015

Strand: Reverse

Name: gdhA [H]

Synonym: PM0043

Alternate gene names: 15601908

Gene position: 47015-45666 (Counterclockwise)

Preceding gene: 15601909

Following gene: 15601906

Centisome position: 2.08

GC content: 44.81

Gene sequence:

>1350_bases
ATGTCTCAAGTTGCAACATTAGATGCGTTTTTAGAAAGAGTCGCACAACGCGACGGTACGCAACCTGAATTTTTACAGGC
AGTACGTGAAGTATTTACCTCTATCTGGCCTTTTTTAGAAGCTAACCCAAAATATCGTTCACAAGCCCTACTCGAACGCT
TAGTTGAACCTGAACGTGTTATTCAGTTCCGCGTAGCATGGACAGATGATCAAGGTCAAACCCAAGTTAACCGTGCATTC
CGCGTGCAATACAACAGTGCAATCGGTCCTTTCAAAGGAGGAATGCGCTTCCATCCTTCTGTTAATCTTTCCATTTTAAA
ATTCTTAGGTTTTGAACAAATCTTCAAAAACGCGTTAACCACATTACCAATGGGGGGCGGCAAAGGCGGTTCCGATTTTG
ATCCTAAAGGAAAATCTGACGGTGAAGTCATGCGTTTCTGCCAAGCTTTAATGGCAGAATTATACCGTCATGTTGGCGCC
GATACCGATGTGCCTGCGGGTGATATTGGTGTAGGAGGACGTGAAGTCGGCTATCTAGCAGGTTATATGAAAAAATTATC
TAACCAAGCCGCTTGCGTGTTTACCGGTCGTGGCTTGTCATTCGGTGGCAGTTTAATTCGCCCAGAAGCAACCGGTTATG
GTCTAGTCTATTTCGCCCAAGCGATGCTCGCTGAAAAAGGGCAAAGCTTTGCCGGTAAAACGGTGTCTGTTTCAGGTTCG
GGTAACGTGGCGCAATATGCCATTGAAAAAGCGCTGCAATTGGGGGCAAAAGTCGTAACTTGTTCTGACTCATCTGGTTA
TGTTTATGATGAAGCCGGTTTCACAACAGAAAAATTGGCTGCATTAATGGAATTGAAAAACGAAAAACGCGGTCGTGTGG
AAGAATACGCGAAACAATTCGGTTTAAAATACGTAGCAGGCAAAACGCCTTGGGAAGTCAAAGTTGATATTGCACTGCCT
TGTGCGACACAAAACGAATTAGACTTAGCGTCTGCTAATACCTTAATTGCCAATGGCGTACAATTAGTGGCTGAAGGGGC
TAACATGCCAACTACTATTGAAGCAACAGATGCCTTTTTAGACGCGGGCGTGCTCTTTGGTCCGGGTAAAGCGGCGAATG
CTGGCGGTGTAGCAACCTCAGGACTAGAAATGACGCAAAGTTCACAACGTTTATACTGGTCTGCGGAAGAAGTCGATAAA
AAACTCCACAGTATCATGTTAGATATTCACGCGAACTGTAAAAAATACGGCACGATTGAAGGTCAAGCCAACATTAATTA
TGTCGTTGGTGCAAACGTTGCGGGATTTGTCAAAGTCGCCGATGCGATGTTAGCGCAAGGTGTTTACTAA

Upstream 100 bases:

>100_bases
CGCCTTCACTTCTGTTGAATACTCTTTCCTGTGCCTAAGTGGCAAATTATTATAATAAATCGCAAACCAATTAAAACATC
ATATCTAGAAGGAAATCATT

Downstream 100 bases:

>100_bases
GCTAAAATAAAAAAACATAGCAAAAAAACACCGCACTTTGTTCGTCAAGAAAGGCCCCAAGTGCGGTGTTTTTTTATTGA
TGTGAGGATGTCTGTCCGTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSQVATLDAFLERVAQRDGTQPEFLQAVREVFTSIWPFLEANPKYRSQALLERLVEPERVIQFRVAWTDDQGQTQVNRAF
RVQYNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMAELYRHVGA
DTDVPAGDIGVGGREVGYLAGYMKKLSNQAACVFTGRGLSFGGSLIRPEATGYGLVYFAQAMLAEKGQSFAGKTVSVSGS
GNVAQYAIEKALQLGAKVVTCSDSSGYVYDEAGFTTEKLAALMELKNEKRGRVEEYAKQFGLKYVAGKTPWEVKVDIALP
CATQNELDLASANTLIANGVQLVAEGANMPTTIEATDAFLDAGVLFGPGKAANAGGVATSGLEMTQSSQRLYWSAEEVDK
KLHSIMLDIHANCKKYGTIEGQANINYVVGANVAGFVKVADAMLAQGVY

Sequences:

>Translated_449_residues
MSQVATLDAFLERVAQRDGTQPEFLQAVREVFTSIWPFLEANPKYRSQALLERLVEPERVIQFRVAWTDDQGQTQVNRAF
RVQYNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMAELYRHVGA
DTDVPAGDIGVGGREVGYLAGYMKKLSNQAACVFTGRGLSFGGSLIRPEATGYGLVYFAQAMLAEKGQSFAGKTVSVSGS
GNVAQYAIEKALQLGAKVVTCSDSSGYVYDEAGFTTEKLAALMELKNEKRGRVEEYAKQFGLKYVAGKTPWEVKVDIALP
CATQNELDLASANTLIANGVQLVAEGANMPTTIEATDAFLDAGVLFGPGKAANAGGVATSGLEMTQSSQRLYWSAEEVDK
KLHSIMLDIHANCKKYGTIEGQANINYVVGANVAGFVKVADAMLAQGVY
>Mature_448_residues
SQVATLDAFLERVAQRDGTQPEFLQAVREVFTSIWPFLEANPKYRSQALLERLVEPERVIQFRVAWTDDQGQTQVNRAFR
VQYNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQALMAELYRHVGAD
TDVPAGDIGVGGREVGYLAGYMKKLSNQAACVFTGRGLSFGGSLIRPEATGYGLVYFAQAMLAEKGQSFAGKTVSVSGSG
NVAQYAIEKALQLGAKVVTCSDSSGYVYDEAGFTTEKLAALMELKNEKRGRVEEYAKQFGLKYVAGKTPWEVKVDIALPC
ATQNELDLASANTLIANGVQLVAEGANMPTTIEATDAFLDAGVLFGPGKAANAGGVATSGLEMTQSSQRLYWSAEEVDKK
LHSIMLDIHANCKKYGTIEGQANINYVVGANVAGFVKVADAMLAQGVY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=367, Percent_Identity=29.700272479564, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4885281, Length=414, Percent_Identity=28.5024154589372, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1788059, Length=448, Percent_Identity=74.7767857142857, Blast_Score=695, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=335, Percent_Identity=29.8507462686567, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=53.2008830022075, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=53.3039647577093, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45549226, Length=346, Percent_Identity=30.0578034682081, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45553475, Length=346, Percent_Identity=30.0578034682081, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24649283, Length=352, Percent_Identity=28.9772727272727, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=29.9319727891156, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48313; Mature: 48182

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVATLDAFLERVAQRDGTQPEFLQAVREVFTSIWPFLEANPKYRSQALLERLVEPERV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCE
IQFRVAWTDDQGQTQVNRAFRVQYNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALT
EEEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMAELYRHVGADTDVPAGDIGVGGREVGYLA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GYMKKLSNQAACVFTGRGLSFGGSLIRPEATGYGLVYFAQAMLAEKGQSFAGKTVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
GNVAQYAIEKALQLGAKVVTCSDSSGYVYDEAGFTTEKLAALMELKNEKRGRVEEYAKQF
CCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHC
GLKYVAGKTPWEVKVDIALPCATQNELDLASANTLIANGVQLVAEGANMPTTIEATDAFL
CCEEECCCCCCEEEEEEEECCCCCCCCCHHHCCHHHHCCHHEEECCCCCCCEEEHHHHHH
DAGVLFGPGKAANAGGVATSGLEMTQSSQRLYWSAEEVDKKLHSIMLDIHANCKKYGTIE
HCCEEECCCCCCCCCCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHHHHHCCCHHCCCCC
GQANINYVVGANVAGFVKVADAMLAQGVY
CCCCEEEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQVATLDAFLERVAQRDGTQPEFLQAVREVFTSIWPFLEANPKYRSQALLERLVEPERV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCE
IQFRVAWTDDQGQTQVNRAFRVQYNSAIGPFKGGMRFHPSVNLSILKFLGFEQIFKNALT
EEEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCEEECCCCCHHHHHHHCHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSDGEVMRFCQALMAELYRHVGADTDVPAGDIGVGGREVGYLA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GYMKKLSNQAACVFTGRGLSFGGSLIRPEATGYGLVYFAQAMLAEKGQSFAGKTVSVSGS
HHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
GNVAQYAIEKALQLGAKVVTCSDSSGYVYDEAGFTTEKLAALMELKNEKRGRVEEYAKQF
CCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHC
GLKYVAGKTPWEVKVDIALPCATQNELDLASANTLIANGVQLVAEGANMPTTIEATDAFL
CCEEECCCCCCEEEEEEEECCCCCCCCCHHHCCHHHHCCHHEEECCCCCCCEEEHHHHHH
DAGVLFGPGKAANAGGVATSGLEMTQSSQRLYWSAEEVDKKLHSIMLDIHANCKKYGTIE
HCCEEECCCCCCCCCCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHHHHHCCCHHCCCCC
GQANINYVVGANVAGFVKVADAMLAQGVY
CCCCEEEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]