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The map label for this gene is truB [H]

Identifier: 15595148

GI number: 15595148

Start: 847491

End: 848339

Strand: Direct

Name: truB [H]

Synonym: BB0803

Alternate gene names: 15595148

Gene position: 847491-848339 (Clockwise)

Preceding gene: 15595147

Following gene: 15595149

Centisome position: 93.06

GC content: 27.44

Gene sequence:

>849_bases
TTGGAAAATGGATTCCTTTTAATTAATAAAGAACAAGGTAAAACTTCTTTTGAAACTCTTTTCCCTATAAAAAAATATTT
TAATACAAATCGTGTTGGGCATGCTGGCACACTTGATAAATTTGCAAGTGGAATTTTGGTTTGTCTTGTGGGAAAATACA
CAAAGCTTTCAGGTTATTTTACTTCTTTAGATAAAGAGTATGTAGCAGAATTCAGATTTGGATTAGAAACAGATACCCTT
GATCCGAATGGAAGAATAGTTAGTAAAACAGATTATATTCCTAATGTAGAGGATATAGATTTAAAGCTTAAAGATTTTGT
AGGAGAGATTTATCAAAGTCCTCCTAGATTTTCTTCTGTTCATATTGATGGCAGCAGAGCCTATAAACTTGCTTTGAATG
GAAAGTTTTTTGAAATTAAAAAACGAAAAGTCACTGTTTATAATATTCAAAGATTAAGTTATGATTTTAGTTCTTCTTTG
CTTAGTTTGAAAATTAGCTGTTCAAAAGGTACTTATATTAGGAGTATTGCAAGAGATTTGGCATATTCTCTAAATTCTTG
CGCGTATGTTAGTAATTTAAAAAGGACTAAAGTAGGTATGTTTAGATTAAAAGATTCCACATTGTGTGAAAATTTGAGTA
AAGCTTCCTTAATTAGTCTAGAATCTTTAAAAAGTTTTGAAAAGGTTTACATTGATTCTAATAAGATAAATCTTGTTAAA
AATGGTGTTTATTTTGAAATTGAAATCAATATTAATGAATTTAAAATTTTAAAATCCAGAGAAGAGAAAATATTAGCAGT
AATTCAAGGAGTGGGTTTGAATAAATATAAATATGTCATTATATTTTAA

Upstream 100 bases:

>100_bases
GCACTCCCAAATTATTATTTGTTAAGGATGATTCTCTTTCCAAATCATTTTATGTTAATAAGTTAATTGAAGGATTAAAT
ACTACAAGAGAGAATTAAAT

Downstream 100 bases:

>100_bases
TTATAAATATATTATGACATTTTAATTTTATATTTGTTTTTTTAAACAATTTAATAATTTATTATTAAAAATAACCCGAA
AAGGATAAATTGCTTTTGTT

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNRVGHAGTLDKFASGILVCLVGKYTKLSGYFTSLDKEYVAEFRFGLETDTL
DPNGRIVSKTDYIPNVEDIDLKLKDFVGEIYQSPPRFSSVHIDGSRAYKLALNGKFFEIKKRKVTVYNIQRLSYDFSSSL
LSLKISCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKVGMFRLKDSTLCENLSKASLISLESLKSFEKVYIDSNKINLVK
NGVYFEIEININEFKILKSREEKILAVIQGVGLNKYKYVIIF

Sequences:

>Translated_282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNRVGHAGTLDKFASGILVCLVGKYTKLSGYFTSLDKEYVAEFRFGLETDTL
DPNGRIVSKTDYIPNVEDIDLKLKDFVGEIYQSPPRFSSVHIDGSRAYKLALNGKFFEIKKRKVTVYNIQRLSYDFSSSL
LSLKISCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKVGMFRLKDSTLCENLSKASLISLESLKSFEKVYIDSNKINLVK
NGVYFEIEININEFKILKSREEKILAVIQGVGLNKYKYVIIF
>Mature_282_residues
MENGFLLINKEQGKTSFETLFPIKKYFNTNRVGHAGTLDKFASGILVCLVGKYTKLSGYFTSLDKEYVAEFRFGLETDTL
DPNGRIVSKTDYIPNVEDIDLKLKDFVGEIYQSPPRFSSVHIDGSRAYKLALNGKFFEIKKRKVTVYNIQRLSYDFSSSL
LSLKISCSKGTYIRSIARDLAYSLNSCAYVSNLKRTKVGMFRLKDSTLCENLSKASLISLESLKSFEKVYIDSNKINLVK
NGVYFEIEININEFKILKSREEKILAVIQGVGLNKYKYVIIF

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=227, Percent_Identity=36.5638766519824, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI215599015, Length=208, Percent_Identity=24.5192307692308, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503337, Length=208, Percent_Identity=24.5192307692308, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367200, Length=212, Percent_Identity=35.8490566037736, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=24.5, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=35.4330708661417, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=208, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364189, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32146; Mature: 32146

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENGFLLINKEQGKTSFETLFPIKKYFNTNRVGHAGTLDKFASGILVCLVGKYTKLSGYF
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCCHHH
TSLDKEYVAEFRFGLETDTLDPNGRIVSKTDYIPNVEDIDLKLKDFVGEIYQSPPRFSSV
HHCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHCCCCCCEEE
HIDGSRAYKLALNGKFFEIKKRKVTVYNIQRLSYDFSSSLLSLKISCSKGTYIRSIARDL
EECCCEEEEEEECCEEEEEEEEEEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHH
AYSLNSCAYVSNLKRTKVGMFRLKDSTLCENLSKASLISLESLKSFEKVYIDSNKINLVK
HHHHCCHHHHHCCCHHEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEE
NGVYFEIEININEFKILKSREEKILAVIQGVGLNKYKYVIIF
CCEEEEEEEECCCEEEEHHHHHHEEHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MENGFLLINKEQGKTSFETLFPIKKYFNTNRVGHAGTLDKFASGILVCLVGKYTKLSGYF
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCCHHH
TSLDKEYVAEFRFGLETDTLDPNGRIVSKTDYIPNVEDIDLKLKDFVGEIYQSPPRFSSV
HHCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHHHHCCCCCCEEE
HIDGSRAYKLALNGKFFEIKKRKVTVYNIQRLSYDFSSSLLSLKISCSKGTYIRSIARDL
EECCCEEEEEEECCEEEEEEEEEEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHH
AYSLNSCAYVSNLKRTKVGMFRLKDSTLCENLSKASLISLESLKSFEKVYIDSNKINLVK
HHHHCCHHHHHCCCHHEEEEEEECCHHHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEE
NGVYFEIEININEFKILKSREEKILAVIQGVGLNKYKYVIIF
CCEEEEEEEECCCEEEEHHHHHHEEHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA