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The map label for this gene is trmD

Identifier: 15595043

GI number: 15595043

Start: 737709

End: 738428

Strand: Direct

Name: trmD

Synonym: BB0698

Alternate gene names: 15595043

Gene position: 737709-738428 (Clockwise)

Preceding gene: 15595042

Following gene: 15595044

Centisome position: 81.0

GC content: 31.81

Gene sequence:

>720_bases
ATGAAATTTACGGTTTTATCCCTTTTTCCAGCAATAATTAAGCCATTTTTTGAAAATTCAATAATGAAAAAAGCTATTAA
TAAGGGAATAGTAAGTTTTGAGCTTGTTGATGTTAGAGATTTTTCAAAAGATAAGCATAAAAGATGTGATGATTTGCCTT
ATGGAGGCGGTGCTGGGATGGTATTGAAGGCCGAACCGATTTCTTTTGCTCTTGAGCATGTAGAGTCTGCCAAGAAAACG
ACAATATTTTTAAGTCCTTCTGGGATAAAGTATAGCCAAGAGTTGGCGTATTCCTTGTCAAAAAGAGAAGAAATTGTTAT
AATTTGTGGAAGATATGAAGGAATTGATCAACGTATTATAGATTTGTATGTTGATTTTGAGATTTCTATTGGAGATTATG
TTTTATCTTCAGGAGAGATTGCAGCTCTTGTTTTAATAGATAGTGTATATAGGTTGCTAGATGGAGTAATAAATCCCAAT
TCTTTATTAGAAGAATCATTTGGTGTAAAAAATGGATTGCTTGAATATCCTCATTATACCAGGCCCTATGATTTTAAGGG
GATAAAGGTCCCAGAAGTTCTTCTTTCGGGACATCATGCAAATATAAAGAATTGGAGGCTTGTTAAAGCTAGAGAAAAAA
CTAAGAAAAATAGATATGATTTATACCTTAAATATTTAGAGATAATAGGAGAAGATAATGGATTTGATAAGAAAAATTGA

Upstream 100 bases:

>100_bases
TGGGTATTAAATTTTTCTTTATTCCCTTTTTAAGTATATATATTGGAGATATAAATACTCAAGAAAAAACAATTGAGCTT
AAGGTTCTAGATCTTTTAAA

Downstream 100 bases:

>100_bases
AGCTCAGAATAAGAAAAATGAGGCTTTTGTTTTTAATGTGGGAGATACTGTGAGGGTTGTTTACAAAATTATTGAGGGTA
GTAATGAAAGGTTGCAGAGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKFTVLSLFPAIIKPFFENSIMKKAINKGIVSFELVDVRDFSKDKHKRCDDLPYGGGAGMVLKAEPISFALEHVESAKKT
TIFLSPSGIKYSQELAYSLSKREEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGVKNGLLEYPHYTRPYDFKGIKVPEVLLSGHHANIKNWRLVKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN

Sequences:

>Translated_239_residues
MKFTVLSLFPAIIKPFFENSIMKKAINKGIVSFELVDVRDFSKDKHKRCDDLPYGGGAGMVLKAEPISFALEHVESAKKT
TIFLSPSGIKYSQELAYSLSKREEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGVKNGLLEYPHYTRPYDFKGIKVPEVLLSGHHANIKNWRLVKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
>Mature_239_residues
MKFTVLSLFPAIIKPFFENSIMKKAINKGIVSFELVDVRDFSKDKHKRCDDLPYGGGAGMVLKAEPISFALEHVESAKKT
TIFLSPSGIKYSQELAYSLSKREEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGVKNGLLEYPHYTRPYDFKGIKVPEVLLSGHHANIKNWRLVKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=41.3333333333333, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BORBU (O51641)

Other databases:

- EMBL:   AE000783
- PIR:   A70187
- RefSeq:   NP_212832.1
- ProteinModelPortal:   O51641
- SMR:   O51641
- EnsemblBacteria:   EBBORT00000008812
- GeneID:   1195551
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0698
- NMPDR:   fig|224326.1.peg.1082
- TIGR:   BB_0698
- GeneTree:   EBGT00050000007285
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   O51641
- ProtClustDB:   PRK00026
- BioCyc:   BBUR224326:BB_0698-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27180; Mature: 27180

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTVLSLFPAIIKPFFENSIMKKAINKGIVSFELVDVRDFSKDKHKRCDDLPYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCHHHCCCCCCCCCCCCCE
VLKAEPISFALEHVESAKKTTIFLSPSGIKYSQELAYSLSKREEIVIICGRYEGIDQRII
EEEECHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEE
DLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPNSLLEESFGVKNGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCC
RPYDFKGIKVPEVLLSGHHANIKNWRLVKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
CCCCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKFTVLSLFPAIIKPFFENSIMKKAINKGIVSFELVDVRDFSKDKHKRCDDLPYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCHHHCCCCCCCCCCCCCE
VLKAEPISFALEHVESAKKTTIFLSPSGIKYSQELAYSLSKREEIVIICGRYEGIDQRII
EEEECHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEE
DLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPNSLLEESFGVKNGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCC
RPYDFKGIKVPEVLLSGHHANIKNWRLVKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
CCCCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685