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The map label for this gene is fruK

Identifier: 15594975

GI number: 15594975

Start: 661606

End: 662529

Strand: Direct

Name: fruK

Synonym: BB0630

Alternate gene names: 15594975

Gene position: 661606-662529 (Clockwise)

Preceding gene: 15594973

Following gene: 15594981

Centisome position: 72.65

GC content: 31.39

Gene sequence:

>924_bases
TTGATATATACTCTAACACTCAATCCTTCTGTGGATTATAAAATAGTTTTAAAAGAATTTCAGGAAGAAAGTCTTAATTA
TGCTTTAAATAACAATTTTTTTGCTGGCGGTAAGGGAATAAATGTAAGCACCGTTCTTAAAAATTTAGGAAAACCTAGTA
CGGCTTTGGGATTTTTGGGAGGTTTTACGGGTGATTATATAAGATTTTCTCTTGATTCTAGGGGCATAAAAAACGATTTT
ATTAAAATAAAATATGATACAAGATTAAATATTAAAATGATAGCAAATGGCAGAGAAACAGAAATTAATGCCAATTCTCC
AGATATTTCTGAGAATGAATTTGAACTTTTGAAAAATAAACTTAAAAATTTAGCAAATAATAGTACATTAGTGATGTCAG
GAAGTGTTCCCGCAGCTCTTGGTGAGGATGCATACAATGAAATAGCTAATAGCATTTCTAATGATGTTAAGCTTATCATT
GATACCAGTGGCAAACCTTTGCGAAAAATTCTTAGATTAAATCCCTTTTTAATAAAGCCTAATATTTATGAACTTGAAGA
TCTTTTTAATGCTAAATTTGATTCTACAAAAGAATTGATTAAAATCGGAAAAAATCTTGTAGAAAGTGGGGTTCAAAACA
TTATAATTTCCATGGGAAGTGACGGAGCTATTTTTATTGGCGGCAAAAATGTTGCTTTTAGGGCCTTTGTTCCTAAGATT
AATTTTGTTAGCACCATTGGAGCAGGAGACTCTGTGATTGCCGGGTTTGTATATGCTTTTGATAATGGAAGTACTTTAGA
GGATTCATTTAAATTTGGTGTTGCAGCTGGTACAGCTACGGCATTAAAAGGCAATCTTTGTGAATTTCAAGATGTTAAAA
AGATGCTTTGTCAGATTAGGGTTGAGGATATTTACACTTCTTAA

Upstream 100 bases:

>100_bases
AATTTTGCATAATAATCACCTTTATATATTATATAGTGTATATACATTTATTAAATTTTACTATTTATTTAATTGATTTT
ATAAATCAAAGGAGATTATC

Downstream 100 bases:

>100_bases
TATTTTAAATAGAGCTGAAGGATTGATTTTGAAACTTTACTGTAGCTTTTAAAAGCTACAGTATTTAGTTTGCTTAAATT
TTTAATTTTGATTTATATAA

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKPSTALGFLGGFTGDYIRFSLDSRGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDISENEFELLKNKLKNLANNSTLVMSGSVPAALGEDAYNEIANSISNDVKLII
DTSGKPLRKILRLNPFLIKPNIYELEDLFNAKFDSTKELIKIGKNLVESGVQNIIISMGSDGAIFIGGKNVAFRAFVPKI
NFVSTIGAGDSVIAGFVYAFDNGSTLEDSFKFGVAAGTATALKGNLCEFQDVKKMLCQIRVEDIYTS

Sequences:

>Translated_307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKPSTALGFLGGFTGDYIRFSLDSRGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDISENEFELLKNKLKNLANNSTLVMSGSVPAALGEDAYNEIANSISNDVKLII
DTSGKPLRKILRLNPFLIKPNIYELEDLFNAKFDSTKELIKIGKNLVESGVQNIIISMGSDGAIFIGGKNVAFRAFVPKI
NFVSTIGAGDSVIAGFVYAFDNGSTLEDSFKFGVAAGTATALKGNLCEFQDVKKMLCQIRVEDIYTS
>Mature_307_residues
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKPSTALGFLGGFTGDYIRFSLDSRGIKNDF
IKIKYDTRLNIKMIANGRETEINANSPDISENEFELLKNKLKNLANNSTLVMSGSVPAALGEDAYNEIANSISNDVKLII
DTSGKPLRKILRLNPFLIKPNIYELEDLFNAKFDSTKELIKIGKNLVESGVQNIIISMGSDGAIFIGGKNVAFRAFVPKI
NFVSTIGAGDSVIAGFVYAFDNGSTLEDSFKFGVAAGTATALKGNLCEFQDVKKMLCQIRVEDIYTS

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1788493, Length=262, Percent_Identity=31.2977099236641, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788017, Length=301, Percent_Identity=29.9003322259136, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1788491, Length=285, Percent_Identity=25.6140350877193, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): K1PF_BORBU (O51575)

Other databases:

- EMBL:   AE000783
- PIR:   E70178
- RefSeq:   NP_212764.1
- ProteinModelPortal:   O51575
- SMR:   O51575
- EnsemblBacteria:   EBBORT00000008615
- GeneID:   1195481
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0630
- NMPDR:   fig|224326.1.peg.1014
- TIGR:   BB_0630
- GeneTree:   EBGT00050000007434
- HOGENOM:   HBG670529
- OMA:   GIDNTAT
- PhylomeDB:   O51575
- ProtClustDB:   CLSK507718
- BioCyc:   BBUR224326:BB_0630-MONOMER
- BRENDA:   2.7.1.56
- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583
- TIGRFAMs:   TIGR03168
- TIGRFAMs:   TIGR03828

Pfam domain/function: PF00294 PfkB

EC number: =2.7.1.56

Molecular weight: Translated: 33595; Mature: 33595

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKPSTALGFLG
CEEEEEECCCCCHHHHHHHHHHHHCCEEECCCEEECCCCCCHHHHHHHCCCCCHHHHHHC
GFTGDYIRFSLDSRGIKNDFIKIKYDTRLNIKMIANGRETEINANSPDISENEFELLKNK
CCCCCEEEEEECCCCCCCCEEEEEECCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHH
LKNLANNSTLVMSGSVPAALGEDAYNEIANSISNDVKLIIDTSGKPLRKILRLNPFLIKP
HHHHCCCCEEEEECCCCCHHCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEECC
NIYELEDLFNAKFDSTKELIKIGKNLVESGVQNIIISMGSDGAIFIGGKNVAFRAFVPKI
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCEEEEEECCCC
NFVSTIGAGDSVIAGFVYAFDNGSTLEDSFKFGVAAGTATALKGNLCEFQDVKKMLCQIR
CEEEECCCCCHHEEEEEEEECCCCCCCHHHHEEEECCCHHHHCCCCCCHHHHHHHHHHHH
VEDIYTS
HHHHCCC
>Mature Secondary Structure
MIYTLTLNPSVDYKIVLKEFQEESLNYALNNNFFAGGKGINVSTVLKNLGKPSTALGFLG
CEEEEEECCCCCHHHHHHHHHHHHCCEEECCCEEECCCCCCHHHHHHHCCCCCHHHHHHC
GFTGDYIRFSLDSRGIKNDFIKIKYDTRLNIKMIANGRETEINANSPDISENEFELLKNK
CCCCCEEEEEECCCCCCCCEEEEEECCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHH
LKNLANNSTLVMSGSVPAALGEDAYNEIANSISNDVKLIIDTSGKPLRKILRLNPFLIKP
HHHHCCCCEEEEECCCCCHHCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEECC
NIYELEDLFNAKFDSTKELIKIGKNLVESGVQNIIISMGSDGAIFIGGKNVAFRAFVPKI
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCEEEEEECCCC
NFVSTIGAGDSVIAGFVYAFDNGSTLEDSFKFGVAAGTATALKGNLCEFQDVKKMLCQIR
CEEEECCCCCHHEEEEEEEECCCCCCCHHHHEEEECCCHHHHCCCCCCHHHHHHHHHHHH
VEDIYTS
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685