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The map label for this gene is recG

Identifier: 15594926

GI number: 15594926

Start: 596531

End: 598591

Strand: Direct

Name: recG

Synonym: BB0581

Alternate gene names: 15594926

Gene position: 596531-598591 (Clockwise)

Preceding gene: 15594925

Following gene: 15594928

Centisome position: 65.5

GC content: 30.81

Gene sequence:

>2061_bases
ATGTTTTTACATGAGTTTGAATATGAGCTTAAAGGTATAGGTGGTCTTGGTGAGAAAGGGGTTGAAAGGTTAAATAATCT
GCAAATTTTTAATGTTAAAGATCTTATTGAGTTTTTTCCTGTAAAATATGAAGATCGTCAAAATATACAAACTTTTCCAG
ATTTTTCTAAGGTAAAAAGTTGTGATATGATGACGGTGTTCACAGTTCTTGGGCATAAAAAATTTGGGGATAGTTCTAAA
AAAAATTTAAAGTTAACAGTTAAAAGTATAAATGAGGAACCTTTCGAAATTCTGCTTTTCAATAGGGCTTTTTTAGAGAA
TGTTTTTAAAATAGATAAAAAATTTTATATTTATTCTAAATTTACCTATAACGATTATAGTGGTTTATGGAGTTGTTCTA
ATTTTGACAGCGAAGTTTACAGCGACAAGCCTGAAAGGTTTAAAAAAATTCTTCCGGTTTATTCTTTGACAGAGGGATTA
ACGTCCAAGAAAATTTCATTATATGTAAAAGAAGCTCTTGAATATTTTTTTAAGTTTGGGCAAACAGACATTCCTAGATT
TTTAATAGAAAAGTATTCTTTATTATCGTTAAGTGATGCTTTAAAAGAGATTCACTTTCCAAGTTCATTAGAAATGCTTG
AAAAGGCAAAAAAGACATTAATTTACAGAGAAATTTTTTTGCTTCAGTTTTTTTCAAGGTATAGATCTTCTAAGATTCTT
TTTCGAGAAAAAAAAGATTTATCAAAAGATTTGCTTGAAAAGGTTGTCTCGAGCTTGCCCTTTGAGCTTACAGAAGATCA
AAAAATTTCTATTGATGAGATATTCTTTGATCTTAACTCTTCTAAGCCAATGAATAGATTGCTTCAAGGTGATGTTGGAA
GTGGTAAAACCCTTGTTGCCTTGCTTTCAGGACTTCCTTTAATTGAAGCTGGATATCAGGTAGCATTTATGGCCCCTACA
GATCTTTTGGCTCGTCAACATTATGATAATTTATCTAACATATTAGCACCTTTTAACATTTCAATGACTCTTTTGACTGG
TAGTTTAAGAAAGAAGGATAAGGAACAAGCTCTAGAAAGCATTAGAAATGGTACTTCTGGTTTAATAGTTGGAACACATG
CTATTTTTTACGAAAGTACAGAATTTAAAAGATTGGCGTATGTTATCATTGACGAACAGCACAAATTTGGAGTTGTTCAA
AGAGAAGAGCTTAAAAACAAAGGAGAAGGGGTGGATATGCTTTTAATGTCTGCAACACCTATTCCTAGAAGTTTTGCATT
AACACTTTTTGGTGATCTTGAAGTTTCGTTTATTAAAACCTTGCCTAAGGGTCGTTTGCCTATTACTACTTATTTAGCAA
GGCATGGAAATGAAGATAAAGTTTATGAGTTTTTAAGAAAAGAGCTCTTAAAGGGTCATCAGGTTTATTTTGTTTATCCA
TTAATTTCATCTTCAGAAAAATTTGAATTAAAAGATGTTAATAATATGTGTTTGAAATTAAAAGAAGTTTTTGGCGAATA
TGTTGTGGACATGCTTCATTCTAAGTTGCCATCTGATTTGAAAGAAGAAATTATGAAAAATTTTTATTCGAAAAAAGTAG
ATATTTTGGTGGCGACTAGTGTTATTGAGGTTGGAATTGATTGTCCAAATGCAACTTGTATGGTAGTAGAGCATGCTGAG
CGTTTTGGACTTTCTACTTTGCATCAAATTAGAGGTCGTGTTGGTAGAAGCAATTTACAATCTTTTTTCTTTTTGCTATA
TAAAGAGCCTTTGACAAGTGCTGGCAAATTTCGATTAAAAACTATAAAAGAAAATTTGGATGGATTTAAAATAGCAGAAG
AAGATTTAAGGCTAAGGGGGCCTGGTAATTTATTTGGACTTGAACAAGCAGGATATTTGAAATTAAAAATAGCTAATTTT
GTTGATGATAGAGAAATCATAGTCTTAGTTCGAGAAGAGCTTGATTTATTTTTTTATGATAATTCTGCTTACGATAAGCT
TGATATTGATTTACTAGACAATCTTTTTTGTTCTTATTTGAATGCCGGAAGAAGTATTTAG

Upstream 100 bases:

>100_bases
TGTTGGGAGTGCTTTAATGGTAGCATTTATAATTATTATTGAACAAGTTGTATTTGCTATTAGAGCTTTAGGAACATATT
TGCCTTTTTAGGAGATTAAA

Downstream 100 bases:

>100_bases
TTTGCATATTTTTCAATTAGGTTAATAAGATTTGTTTTGTTCTGGTTCCAAAATTCAAGAAGCTTATGTTGTAAATTATT
AAAATATTTTTTCTGAATAA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MFLHEFEYELKGIGGLGEKGVERLNNLQIFNVKDLIEFFPVKYEDRQNIQTFPDFSKVKSCDMMTVFTVLGHKKFGDSSK
KNLKLTVKSINEEPFEILLFNRAFLENVFKIDKKFYIYSKFTYNDYSGLWSCSNFDSEVYSDKPERFKKILPVYSLTEGL
TSKKISLYVKEALEYFFKFGQTDIPRFLIEKYSLLSLSDALKEIHFPSSLEMLEKAKKTLIYREIFLLQFFSRYRSSKIL
FREKKDLSKDLLEKVVSSLPFELTEDQKISIDEIFFDLNSSKPMNRLLQGDVGSGKTLVALLSGLPLIEAGYQVAFMAPT
DLLARQHYDNLSNILAPFNISMTLLTGSLRKKDKEQALESIRNGTSGLIVGTHAIFYESTEFKRLAYVIIDEQHKFGVVQ
REELKNKGEGVDMLLMSATPIPRSFALTLFGDLEVSFIKTLPKGRLPITTYLARHGNEDKVYEFLRKELLKGHQVYFVYP
LISSSEKFELKDVNNMCLKLKEVFGEYVVDMLHSKLPSDLKEEIMKNFYSKKVDILVATSVIEVGIDCPNATCMVVEHAE
RFGLSTLHQIRGRVGRSNLQSFFFLLYKEPLTSAGKFRLKTIKENLDGFKIAEEDLRLRGPGNLFGLEQAGYLKLKIANF
VDDREIIVLVREELDLFFYDNSAYDKLDIDLLDNLFCSYLNAGRSI

Sequences:

>Translated_686_residues
MFLHEFEYELKGIGGLGEKGVERLNNLQIFNVKDLIEFFPVKYEDRQNIQTFPDFSKVKSCDMMTVFTVLGHKKFGDSSK
KNLKLTVKSINEEPFEILLFNRAFLENVFKIDKKFYIYSKFTYNDYSGLWSCSNFDSEVYSDKPERFKKILPVYSLTEGL
TSKKISLYVKEALEYFFKFGQTDIPRFLIEKYSLLSLSDALKEIHFPSSLEMLEKAKKTLIYREIFLLQFFSRYRSSKIL
FREKKDLSKDLLEKVVSSLPFELTEDQKISIDEIFFDLNSSKPMNRLLQGDVGSGKTLVALLSGLPLIEAGYQVAFMAPT
DLLARQHYDNLSNILAPFNISMTLLTGSLRKKDKEQALESIRNGTSGLIVGTHAIFYESTEFKRLAYVIIDEQHKFGVVQ
REELKNKGEGVDMLLMSATPIPRSFALTLFGDLEVSFIKTLPKGRLPITTYLARHGNEDKVYEFLRKELLKGHQVYFVYP
LISSSEKFELKDVNNMCLKLKEVFGEYVVDMLHSKLPSDLKEEIMKNFYSKKVDILVATSVIEVGIDCPNATCMVVEHAE
RFGLSTLHQIRGRVGRSNLQSFFFLLYKEPLTSAGKFRLKTIKENLDGFKIAEEDLRLRGPGNLFGLEQAGYLKLKIANF
VDDREIIVLVREELDLFFYDNSAYDKLDIDLLDNLFCSYLNAGRSI
>Mature_686_residues
MFLHEFEYELKGIGGLGEKGVERLNNLQIFNVKDLIEFFPVKYEDRQNIQTFPDFSKVKSCDMMTVFTVLGHKKFGDSSK
KNLKLTVKSINEEPFEILLFNRAFLENVFKIDKKFYIYSKFTYNDYSGLWSCSNFDSEVYSDKPERFKKILPVYSLTEGL
TSKKISLYVKEALEYFFKFGQTDIPRFLIEKYSLLSLSDALKEIHFPSSLEMLEKAKKTLIYREIFLLQFFSRYRSSKIL
FREKKDLSKDLLEKVVSSLPFELTEDQKISIDEIFFDLNSSKPMNRLLQGDVGSGKTLVALLSGLPLIEAGYQVAFMAPT
DLLARQHYDNLSNILAPFNISMTLLTGSLRKKDKEQALESIRNGTSGLIVGTHAIFYESTEFKRLAYVIIDEQHKFGVVQ
REELKNKGEGVDMLLMSATPIPRSFALTLFGDLEVSFIKTLPKGRLPITTYLARHGNEDKVYEFLRKELLKGHQVYFVYP
LISSSEKFELKDVNNMCLKLKEVFGEYVVDMLHSKLPSDLKEEIMKNFYSKKVDILVATSVIEVGIDCPNATCMVVEHAE
RFGLSTLHQIRGRVGRSNLQSFFFLLYKEPLTSAGKFRLKTIKENLDGFKIAEEDLRLRGPGNLFGLEQAGYLKLKIANF
VDDREIIVLVREELDLFFYDNSAYDKLDIDLLDNLFCSYLNAGRSI

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367254, Length=661, Percent_Identity=33.7367624810893, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1787357, Length=413, Percent_Identity=34.3825665859564, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECG_BORBU (O51528)

Other databases:

- EMBL:   AE000783
- PIR:   D70172
- RefSeq:   NP_212715.1
- ProteinModelPortal:   O51528
- SMR:   O51528
- EnsemblBacteria:   EBBORT00000009129
- GeneID:   1195428
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0581
- NMPDR:   fig|224326.1.peg.965
- TIGR:   BB_0581
- GeneTree:   EBGT00050000006755
- HOGENOM:   HBG747311
- OMA:   SKYICFN
- ProtClustDB:   PRK10917
- BioCyc:   BBUR224326:BB_0581-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00643

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; SSF50249 Nucleic_acid_OB

EC number: =3.6.4.12

Molecular weight: Translated: 79045; Mature: 79045

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLHEFEYELKGIGGLGEKGVERLNNLQIFNVKDLIEFFPVKYEDRQNIQTFPDFSKVKS
CCCCCCCHHHCCCCCCCHHHHHHHHCEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
CDMMTVFTVLGHKKFGDSSKKNLKLTVKSINEEPFEILLFNRAFLENVFKIDKKFYIYSK
CCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHCCEEEEEEE
FTYNDYSGLWSCSNFDSEVYSDKPERFKKILPVYSLTEGLTSKKISLYVKEALEYFFKFG
EEECCCCCCEECCCCCCHHHCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCC
QTDIPRFLIEKYSLLSLSDALKEIHFPSSLEMLEKAKKTLIYREIFLLQFFSRYRSSKIL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FREKKDLSKDLLEKVVSSLPFELTEDQKISIDEIFFDLNSSKPMNRLLQGDVGSGKTLVA
EHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHEEECCCCCHHHHHHCCCCCCCHHHHH
LLSGLPLIEAGYQVAFMAPTDLLARQHYDNLSNILAPFNISMTLLTGSLRKKDKEQALES
HHCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHH
IRNGTSGLIVGTHAIFYESTEFKRLAYVIIDEQHKFGVVQREELKNKGEGVDMLLMSATP
HHCCCCCEEEEEEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHHCCCCCCEEEEEECCC
IPRSFALTLFGDLEVSFIKTLPKGRLPITTYLARHGNEDKVYEFLRKELLKGHQVYFVYP
CCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEE
LISSSEKFELKDVNNMCLKLKEVFGEYVVDMLHSKLPSDLKEEIMKNFYSKKVDILVATS
EECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEH
VIEVGIDCPNATCMVVEHAERFGLSTLHQIRGRVGRSNLQSFFFLLYKEPLTSAGKFRLK
HHHHCCCCCCCEEEEEECHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEHH
TIKENLDGFKIAEEDLRLRGPGNLFGLEQAGYLKLKIANFVDDREIIVLVREELDLFFYD
HHHHCCCCCEEECCCEEECCCCCEECCCCCCEEEEEEECCCCCCEEEEEEEECCEEEEEC
NSAYDKLDIDLLDNLFCSYLNAGRSI
CCCCCEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLHEFEYELKGIGGLGEKGVERLNNLQIFNVKDLIEFFPVKYEDRQNIQTFPDFSKVKS
CCCCCCCHHHCCCCCCCHHHHHHHHCEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
CDMMTVFTVLGHKKFGDSSKKNLKLTVKSINEEPFEILLFNRAFLENVFKIDKKFYIYSK
CCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHCCEEEEEEE
FTYNDYSGLWSCSNFDSEVYSDKPERFKKILPVYSLTEGLTSKKISLYVKEALEYFFKFG
EEECCCCCCEECCCCCCHHHCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHCC
QTDIPRFLIEKYSLLSLSDALKEIHFPSSLEMLEKAKKTLIYREIFLLQFFSRYRSSKIL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FREKKDLSKDLLEKVVSSLPFELTEDQKISIDEIFFDLNSSKPMNRLLQGDVGSGKTLVA
EHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHEEECCCCCHHHHHHCCCCCCCHHHHH
LLSGLPLIEAGYQVAFMAPTDLLARQHYDNLSNILAPFNISMTLLTGSLRKKDKEQALES
HHCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHH
IRNGTSGLIVGTHAIFYESTEFKRLAYVIIDEQHKFGVVQREELKNKGEGVDMLLMSATP
HHCCCCCEEEEEEEEEECCCCCCEEEEEEEECCCCCCCEEHHHHHCCCCCCEEEEEECCC
IPRSFALTLFGDLEVSFIKTLPKGRLPITTYLARHGNEDKVYEFLRKELLKGHQVYFVYP
CCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEE
LISSSEKFELKDVNNMCLKLKEVFGEYVVDMLHSKLPSDLKEEIMKNFYSKKVDILVATS
EECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEH
VIEVGIDCPNATCMVVEHAERFGLSTLHQIRGRVGRSNLQSFFFLLYKEPLTSAGKFRLK
HHHHCCCCCCCEEEEEECHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEHH
TIKENLDGFKIAEEDLRLRGPGNLFGLEQAGYLKLKIANFVDDREIIVLVREELDLFFYD
HHHHCCCCCEEECCCEEECCCCCEECCCCCCEEEEEEECCCCCCEEEEEEEECCEEEEEC
NSAYDKLDIDLLDNLFCSYLNAGRSI
CCCCCEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685