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The map label for this gene is exoA [H]

Identifier: 15594879

GI number: 15594879

Start: 543049

End: 543816

Strand: Direct

Name: exoA [H]

Synonym: BB0534

Alternate gene names: 15594879

Gene position: 543049-543816 (Clockwise)

Preceding gene: 15594878

Following gene: 15594880

Centisome position: 59.63

GC content: 31.12

Gene sequence:

>768_bases
ATGAAATTAATTTCATGGAATGTAAATGGAATTAGAGCTGTTTTAAAGAAAGGTTTTCTTGAGTTTGTAAAAGAATATAC
TCCAGATATTTTATGTATTCAAGAAACTAAAGCCTTAAGAGAACAGTTGCCAAAGGATTTAATTCTTGAAAATTATTATT
CTTATTTTTCAAATTCAAAGATTAAAGGTTATAGTGGTGTTTGCATTTATTCAAAAGTTGAGCCTATCTCTGTAAGGCTG
CTTGGAGAAGAAATTTTTGACAATGAGGGTAGGGGGCTTGTAGCATGCTACGACGATTTTATATTGGTTAACGGTTATTT
CCCCAATTCTCAAGTTTTAAGAAGAAGACTTGGGTATAAACTTGATTTTTTATCTTATGTTGAAAATCTTGCAGATTCTC
TTGTATGTGATGGTAAAAATGTAGTAATTTGTGGTGATTTTAATATTGCTCATACCGAGATTGACCTTATAAGTCCCGAT
TCTAATAGAGACTCTCCGGGATATTATATTGAAGAGACTACTTGGTTAGATGATTTTTTAAACAAAGGATATGTGGATAC
ATTTAGGATATTTAATAAGGATCCTGGTTATTATACTTGGTGGAGCTATAGAACAAGAGCTAGAGAGAGAAATATGGGGT
GGAGAATTGATTATTTTATTGTTAATGAATTTTTCAAGAGAAATGTTGAAAAATCTCTAATTTTAGACAAAGTAATGGGA
AGTGATCATTGCCCTGTTTTTTTAGAGTTGGCTGATGTAGTTAGCTAA

Upstream 100 bases:

>100_bases
AATGCATGAAGAATTTGATTATTTAGAAAAAGATAATATCTATTTAGCTTATGATGGATTAAAGATTTATATTTGATTTA
ACTTTAGTTAGAGAGGATTT

Downstream 100 bases:

>100_bases
AGGGAGGAGGCTAGATTGAAAAAAAACATTGCAATTTTTTATTTAATATTTATTGTTAATTTTAGTTTTGGAATTGATTT
AAATGATTTTGATTTTTACC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCIQETKALREQLPKDLILENYYSYFSNSKIKGYSGVCIYSKVEPISVRL
LGEEIFDNEGRGLVACYDDFILVNGYFPNSQVLRRRLGYKLDFLSYVENLADSLVCDGKNVVICGDFNIAHTEIDLISPD
SNRDSPGYYIEETTWLDDFLNKGYVDTFRIFNKDPGYYTWWSYRTRARERNMGWRIDYFIVNEFFKRNVEKSLILDKVMG
SDHCPVFLELADVVS

Sequences:

>Translated_255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCIQETKALREQLPKDLILENYYSYFSNSKIKGYSGVCIYSKVEPISVRL
LGEEIFDNEGRGLVACYDDFILVNGYFPNSQVLRRRLGYKLDFLSYVENLADSLVCDGKNVVICGDFNIAHTEIDLISPD
SNRDSPGYYIEETTWLDDFLNKGYVDTFRIFNKDPGYYTWWSYRTRARERNMGWRIDYFIVNEFFKRNVEKSLILDKVMG
SDHCPVFLELADVVS
>Mature_255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCIQETKALREQLPKDLILENYYSYFSNSKIKGYSGVCIYSKVEPISVRL
LGEEIFDNEGRGLVACYDDFILVNGYFPNSQVLRRRLGYKLDFLSYVENLADSLVCDGKNVVICGDFNIAHTEIDLISPD
SNRDSPGYYIEETTWLDDFLNKGYVDTFRIFNKDPGYYTWWSYRTRARERNMGWRIDYFIVNEFFKRNVEKSLILDKVMG
SDHCPVFLELADVVS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=39.5437262357414, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=39.5437262357414, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=39.5437262357414, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI18375507, Length=311, Percent_Identity=30.2250803858521, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=28.1954887218045, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=33.852140077821, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=42.7450980392157, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=42.7450980392157, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29756; Mature: 29756

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCIQETKALREQLPKDLILENYYSYFSNSK
CEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCCCC
IKGYSGVCIYSKVEPISVRLLGEEIFDNEGRGLVACYDDFILVNGYFPNSQVLRRRLGYK
CCCCCCEEEEECCCCEEEEEECHHHHCCCCCEEEEEEECEEEEEECCCCHHHHHHHHCCC
LDFLSYVENLADSLVCDGKNVVICGDFNIAHTEIDLISPDSNRDSPGYYIEETTWLDDFL
CHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEEEEECCCCCCCCCCEEEECCHHHHHHH
NKGYVDTFRIFNKDPGYYTWWSYRTRARERNMGWRIDYFIVNEFFKRNVEKSLILDKVMG
CCCCEEEEEEECCCCCEEEEEHHHHHHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHC
SDHCPVFLELADVVS
CCCCCHHEEHHHHCC
>Mature Secondary Structure
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCIQETKALREQLPKDLILENYYSYFSNSK
CEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHCCCC
IKGYSGVCIYSKVEPISVRLLGEEIFDNEGRGLVACYDDFILVNGYFPNSQVLRRRLGYK
CCCCCCEEEEECCCCEEEEEECHHHHCCCCCEEEEEEECEEEEEECCCCHHHHHHHHCCC
LDFLSYVENLADSLVCDGKNVVICGDFNIAHTEIDLISPDSNRDSPGYYIEETTWLDDFL
CHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEEEEECCCCCCCCCCEEEECCHHHHHHH
NKGYVDTFRIFNKDPGYYTWWSYRTRARERNMGWRIDYFIVNEFFKRNVEKSLILDKVMG
CCCCEEEEEEECCCCCEEEEEHHHHHHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHC
SDHCPVFLELADVVS
CCCCCHHEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]