Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 15594867

GI number: 15594867

Start: 532938

End: 533606

Strand: Direct

Name: nadE [H]

Synonym: BB0522

Alternate gene names: 15594867

Gene position: 532938-533606 (Clockwise)

Preceding gene: 15594866

Following gene: 15594868

Centisome position: 58.52

GC content: 28.4

Gene sequence:

>669_bases
ATGCCTAGTAAATTTTCATCAGCAGGTTCTATTTCTGATGCTAAAGAACTTTCTAGAAAATTAGGTTTTAAATTGATTGA
AATGCCAATAAAAGATTTATTTCAGGTAAGCAGTAGATTTTTTGAAGGATATTTTGATATTAAAGGTGTTACTGGAGAAA
ATTTGCAAGCTCGGTTAAGAGGGCTTTTATTAATGTCTTATAGTAATTCTCAAAATTCTTTGCTTTTAAATACTGGTAAC
AAAAGCGAAATTGCAGTTGGTTATTGTACCCTTTATGGGGATACTTGCGGAGGGCTTGCTTTGATCGGAGATCTTTTTAA
GACTGAGGTTTATGATCTTGTAAATTATATTAATGCAAAATTTGACCAATGTATTATTCCTGTTAATATTATTTTAAAGG
AGCCTTCAGCTGAGTTAAGGTTTGATCAAAAAGATAGTGATTATCTTCCAAAATATGAAGTTCTTGATGTTATTTTAAAT
AGATATTTGATTGGCAATGAATCTGTAGATTCAATTTATCTTAATTTTGAAAAAGGTATAGTTGATAAAGTTTTAAATCT
TTATTTTAAAAATGAATACAAAAGAAGACAGGGAGCTCCTATAGTTAAAGTTTCTGAAAAAACCTTTGGTTTTGAGCTTT
CAATGCCTATTTTAAATAATATAATTTGA

Upstream 100 bases:

>100_bases
TTCCAAAGTTCATTTGGGAGTATCTGGGGGTGTTGATTCTGCTCTTATTGCGTATCTTGCATGTTTTGCTTTGGGCGCTG
AGAATGTTGTTGGGATTTCT

Downstream 100 bases:

>100_bases
AATTATGATTGGAGCTTGAGATTTTGAAATTCTTTTCTTTTTTTAAATTTTATTAATAATATTTTTTGCTGGAAAAATTG
AAAAATAGCTTTTTAAGTTA

Product: NH(3)-dependent NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MPSKFSSAGSISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLRGLLLMSYSNSQNSLLLNTGN
KSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEVLDVILN
RYLIGNESVDSIYLNFEKGIVDKVLNLYFKNEYKRRQGAPIVKVSEKTFGFELSMPILNNII

Sequences:

>Translated_222_residues
MPSKFSSAGSISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLRGLLLMSYSNSQNSLLLNTGN
KSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEVLDVILN
RYLIGNESVDSIYLNFEKGIVDKVLNLYFKNEYKRRQGAPIVKVSEKTFGFELSMPILNNII
>Mature_221_residues
PSKFSSAGSISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLRGLLLMSYSNSQNSLLLNTGNK
SEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEVLDVILNR
YLIGNESVDSIYLNFEKGIVDKVLNLYFKNEYKRRQGAPIVKVSEKTFGFELSMPILNNII

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 25094; Mature: 24963

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKFSSAGSISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLR
CCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHH
GLLLMSYSNSQNSLLLNTGNKSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAK
EEEEEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
FDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEVLDVILNRYLIGNESVDSIYLNFEKGI
CCCEEEEEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
VDKVLNLYFKNEYKRRQGAPIVKVSEKTFGFELSMPILNNII
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHCCC
>Mature Secondary Structure 
PSKFSSAGSISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLR
CCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHH
GLLLMSYSNSQNSLLLNTGNKSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAK
EEEEEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
FDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEVLDVILNRYLIGNESVDSIYLNFEKGI
CCCEEEEEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
VDKVLNLYFKNEYKRRQGAPIVKVSEKTFGFELSMPILNNII
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]