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The map label for this gene is recJ [H]

Identifier: 15594599

GI number: 15594599

Start: 263988

End: 266114

Strand: Direct

Name: recJ [H]

Synonym: BB0254

Alternate gene names: 15594599

Gene position: 263988-266114 (Clockwise)

Preceding gene: 15594597

Following gene: 15594600

Centisome position: 28.99

GC content: 25.53

Gene sequence:

>2127_bases
ATGAAAATTTGGAAACAAAAAGAAACTAATATACAGGCCCAAGAATTAACTCGCATTGCTAAGCAATATAATATCAATGC
CTTTGAGGCAACTTTACTTATAAATCGAGAAATCAAAGAAGAAGATTTTATGTTTTTCCTTGAAGACAGCGTAAATTTAT
TACACAACCCATTTTTATTAAAAAATATAGATAAATTTATAAACAGAATTAATAAAGCAATCAAAAATAATGAAAACATA
TTAATCTTTGGAGATAAAGATGCTGATGGAATCACAGCAACAATAATAATGTACGAAACTCTTAAAGATTTTGGACTTAA
TGTGAGTTATAAAATACCTTCAAATGGAGAGTTTTATGGACTTTCAAACGAATTAATCAACACAGCTTTAGAAAAAAAAA
TCTCTTTAATAATAACTGTTGATAATGGTATTTCTAGCATTGAAGAAATAAATTATGCAAATTCAAAAGGAATAGAAATA
ATAATAACAGACCATCATCTTCCAGGCGAAAATTTTAAAACTGAAAACATAGTTATAAATCCTCACCTAAAAGATGACAA
ATATCCATTCAAAGAAATAGCAGGATGTTGTGTAAGCTTTAAAGCTTGCCTTGCTCTTAGTATGTCCTTTACAGACCTTT
ATTCTAAAAACCTTGTATTTTTATTTTTAGAAAAAACAAAAAATGAAATTATTCTTCACGCAATAGAAATAAACAACTAC
ATTCTAAAACAATATCTAAGATTAAATAATAAAAATGATCCCCTAATTAACTCAAACAAACTAGAAAAATTTGTACAAAA
CAAATGTATAATAATCTTTAACAAAGAAGATCAAAATGAATTATTAAATAAACACTTTGCAAAAAGCATAAACATAAACA
CAATTGATATTAGTGAAAATTTTATAAAAAAATACCCAAACTTTAGAAAAAAAACATTAACAGACTTAATCCAAGCAACC
AAATATTTCAAATACAAAGAAGTTGAGATTAAAGACAAGCTTTACTACATATTTTACAACGTAATTTTTGAAACTAACAA
AAATTTGCTCCAAAAATGCCTAAAAAGACTTAGTTTTGTTGCAATAGGAACAATAGCAGACAATATGCCCATTATTAATG
AAAACAGAATAATCCTAAAAGTAGGACTTAAAGAAATCGCACTAAGAGAGAGAATGTCTATTAATTATCTATTAAAAGAT
GGAAACATATTAACAAAACCAAATATAACTTCAACAGATATTGCATATAAAATTGCACCAATACTAAACTCAACGGGAAG
GCTTGAAAAAGCAGATATTGCAATAAATTTTTTACTAACTAATGACATTAATCAAATAGAAAACAAATTTAAAGAAATAA
AAGAAATCAACGAACTGAGAAAATACAAAGAAGAAAAAGCTTGGAATTCGCATAATAAAAATACTATTTTTAAAAATGAT
AAATTCATAGTTTGCTATGATAACAATACTCCAAAAGGAATAAGTTCTAGAATTGCAACTAGACTTTCTTCTTACTATCA
AAAAGTTGCTATTTTTTTAACAAAGCAAGATAACATTATTAAAGGATCAATTAGATCAAACAATAAAATCAATTCAAAAA
CACTAATATCAATAATACCTTCCCATTTAGTAATAAATTCGGGGGGCCATAAAGCTGCAGCTGGATTTACGCTGCATGAA
AATTTACTCGAAGACTTTATTAAAGAATTAGAATATGCAACTACAAAAGTTGAATATGAAACTACTAATGAAAACGAATC
AATACCAATAGATGCTATTCTTCCAAAAAATTTAACAAAAGATTCTCTTTTCAAAACAATAGAAATATTTGAACCTTATG
GCTATGAATTTAGAGAGCCAATATTATTAATGAAAAATGCTTACCTTCAAGAACTCAAAATAATTGACAAAAATCATAGC
TCAAAACATATAAATATGCGAATTAAATCACAAAACGATTATTATAAAGCTATTTTTTTTAACGGAACAAAAAAAATAGA
AGAATTGGATATAAAAGAAGATCAATATTTAGACATCATTTTTACAGTTAATGAAGATTTTTATTATCCTAGAGAAAAAA
TTTTGAAAATCATAGACATAAAAAAGAATGCTCAAATCAATGTATAG

Upstream 100 bases:

>100_bases
ATAAGCATAATAACTACTACTTAAAATTAATTAATCTATGGTGAAGTTAGTAAAGAATAATCCTTGAAAAATCGATTAAA
AATGAATTCTAGAGAAGTAA

Downstream 100 bases:

>100_bases
AATACAAAAAGCCCTACTTATACTGTGTATTCTAGGAATAGAAAATATAAATTCAGAAATAATAAATACTATTCCTAAAG
TCCAATATAAAAAAGAGGCA

Product: single-stranded-DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MKIWKQKETNIQAQELTRIAKQYNINAFEATLLINREIKEEDFMFFLEDSVNLLHNPFLLKNIDKFINRINKAIKNNENI
LIFGDKDADGITATIIMYETLKDFGLNVSYKIPSNGEFYGLSNELINTALEKKISLIITVDNGISSIEEINYANSKGIEI
IITDHHLPGENFKTENIVINPHLKDDKYPFKEIAGCCVSFKACLALSMSFTDLYSKNLVFLFLEKTKNEIILHAIEINNY
ILKQYLRLNNKNDPLINSNKLEKFVQNKCIIIFNKEDQNELLNKHFAKSININTIDISENFIKKYPNFRKKTLTDLIQAT
KYFKYKEVEIKDKLYYIFYNVIFETNKNLLQKCLKRLSFVAIGTIADNMPIINENRIILKVGLKEIALRERMSINYLLKD
GNILTKPNITSTDIAYKIAPILNSTGRLEKADIAINFLLTNDINQIENKFKEIKEINELRKYKEEKAWNSHNKNTIFKND
KFIVCYDNNTPKGISSRIATRLSSYYQKVAIFLTKQDNIIKGSIRSNNKINSKTLISIIPSHLVINSGGHKAAAGFTLHE
NLLEDFIKELEYATTKVEYETTNENESIPIDAILPKNLTKDSLFKTIEIFEPYGYEFREPILLMKNAYLQELKIIDKNHS
SKHINMRIKSQNDYYKAIFFNGTKKIEELDIKEDQYLDIIFTVNEDFYYPREKILKIIDIKKNAQINV

Sequences:

>Translated_708_residues
MKIWKQKETNIQAQELTRIAKQYNINAFEATLLINREIKEEDFMFFLEDSVNLLHNPFLLKNIDKFINRINKAIKNNENI
LIFGDKDADGITATIIMYETLKDFGLNVSYKIPSNGEFYGLSNELINTALEKKISLIITVDNGISSIEEINYANSKGIEI
IITDHHLPGENFKTENIVINPHLKDDKYPFKEIAGCCVSFKACLALSMSFTDLYSKNLVFLFLEKTKNEIILHAIEINNY
ILKQYLRLNNKNDPLINSNKLEKFVQNKCIIIFNKEDQNELLNKHFAKSININTIDISENFIKKYPNFRKKTLTDLIQAT
KYFKYKEVEIKDKLYYIFYNVIFETNKNLLQKCLKRLSFVAIGTIADNMPIINENRIILKVGLKEIALRERMSINYLLKD
GNILTKPNITSTDIAYKIAPILNSTGRLEKADIAINFLLTNDINQIENKFKEIKEINELRKYKEEKAWNSHNKNTIFKND
KFIVCYDNNTPKGISSRIATRLSSYYQKVAIFLTKQDNIIKGSIRSNNKINSKTLISIIPSHLVINSGGHKAAAGFTLHE
NLLEDFIKELEYATTKVEYETTNENESIPIDAILPKNLTKDSLFKTIEIFEPYGYEFREPILLMKNAYLQELKIIDKNHS
SKHINMRIKSQNDYYKAIFFNGTKKIEELDIKEDQYLDIIFTVNEDFYYPREKILKIIDIKKNAQINV
>Mature_708_residues
MKIWKQKETNIQAQELTRIAKQYNINAFEATLLINREIKEEDFMFFLEDSVNLLHNPFLLKNIDKFINRINKAIKNNENI
LIFGDKDADGITATIIMYETLKDFGLNVSYKIPSNGEFYGLSNELINTALEKKISLIITVDNGISSIEEINYANSKGIEI
IITDHHLPGENFKTENIVINPHLKDDKYPFKEIAGCCVSFKACLALSMSFTDLYSKNLVFLFLEKTKNEIILHAIEINNY
ILKQYLRLNNKNDPLINSNKLEKFVQNKCIIIFNKEDQNELLNKHFAKSININTIDISENFIKKYPNFRKKTLTDLIQAT
KYFKYKEVEIKDKLYYIFYNVIFETNKNLLQKCLKRLSFVAIGTIADNMPIINENRIILKVGLKEIALRERMSINYLLKD
GNILTKPNITSTDIAYKIAPILNSTGRLEKADIAINFLLTNDINQIENKFKEIKEINELRKYKEEKAWNSHNKNTIFKND
KFIVCYDNNTPKGISSRIATRLSSYYQKVAIFLTKQDNIIKGSIRSNNKINSKTLISIIPSHLVINSGGHKAAAGFTLHE
NLLEDFIKELEYATTKVEYETTNENESIPIDAILPKNLTKDSLFKTIEIFEPYGYEFREPILLMKNAYLQELKIIDKNHS
SKHINMRIKSQNDYYKAIFFNGTKKIEELDIKEDQYLDIIFTVNEDFYYPREKILKIIDIKKNAQINV

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=168, Percent_Identity=38.0952380952381, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 82367; Mature: 82367

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIWKQKETNIQAQELTRIAKQYNINAFEATLLINREIKEEDFMFFLEDSVNLLHNPFLL
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEECCHHHHCCCHHH
KNIDKFINRINKAIKNNENILIFGDKDADGITATIIMYETLKDFGLNVSYKIPSNGEFYG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHHHHCCEEEEECCCCCCEEE
LSNELINTALEKKISLIITVDNGISSIEEINYANSKGIEIIITDHHLPGENFKTENIVIN
CHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEC
PHLKDDKYPFKEIAGCCVSFKACLALSMSFTDLYSKNLVFLFLEKTKNEIILHAIEINNY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEHHHH
ILKQYLRLNNKNDPLINSNKLEKFVQNKCIIIFNKEDQNELLNKHFAKSININTIDISEN
HHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCHH
FIKKYPNFRKKTLTDLIQATKYFKYKEVEIKDKLYYIFYNVIFETNKNLLQKCLKRLSFV
HHHHCCCHHHHHHHHHHHHHHHCEEEEEEEHHHEEEEEEEEHHHCCHHHHHHHHHHHHEE
AIGTIADNMPIINENRIILKVGLKEIALRERMSINYLLKDGNILTKPNITSTDIAYKIAP
EEEEECCCCCEECCCEEEEEECHHHHHHHHHCCEEEEEECCCEEECCCCCCCCHHEEEEH
ILNSTGRLEKADIAINFLLTNDINQIENKFKEIKEINELRKYKEEKAWNSHNKNTIFKND
HHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KFIVCYDNNTPKGISSRIATRLSSYYQKVAIFLTKQDNIIKGSIRSNNKINSKTLISIIP
CEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCCCCHHHEEECC
SHLVINSGGHKAAAGFTLHENLLEDFIKELEYATTKVEYETTNENESIPIDAILPKNLTK
CCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECCCCCCH
DSLFKTIEIFEPYGYEFREPILLMKNAYLQELKIIDKNHSSKHINMRIKSQNDYYKAIFF
HHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEE
NGTKKIEELDIKEDQYLDIIFTVNEDFYYPREKILKIIDIKKNAQINV
CCCCCHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHEEECCCCEECC
>Mature Secondary Structure
MKIWKQKETNIQAQELTRIAKQYNINAFEATLLINREIKEEDFMFFLEDSVNLLHNPFLL
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEECCHHHHCCCHHH
KNIDKFINRINKAIKNNENILIFGDKDADGITATIIMYETLKDFGLNVSYKIPSNGEFYG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEHHHHHCCEEEEECCCCCCEEE
LSNELINTALEKKISLIITVDNGISSIEEINYANSKGIEIIITDHHLPGENFKTENIVIN
CHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEC
PHLKDDKYPFKEIAGCCVSFKACLALSMSFTDLYSKNLVFLFLEKTKNEIILHAIEINNY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEHHHH
ILKQYLRLNNKNDPLINSNKLEKFVQNKCIIIFNKEDQNELLNKHFAKSININTIDISEN
HHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCHH
FIKKYPNFRKKTLTDLIQATKYFKYKEVEIKDKLYYIFYNVIFETNKNLLQKCLKRLSFV
HHHHCCCHHHHHHHHHHHHHHHCEEEEEEEHHHEEEEEEEEHHHCCHHHHHHHHHHHHEE
AIGTIADNMPIINENRIILKVGLKEIALRERMSINYLLKDGNILTKPNITSTDIAYKIAP
EEEEECCCCCEECCCEEEEEECHHHHHHHHHCCEEEEEECCCEEECCCCCCCCHHEEEEH
ILNSTGRLEKADIAINFLLTNDINQIENKFKEIKEINELRKYKEEKAWNSHNKNTIFKND
HHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KFIVCYDNNTPKGISSRIATRLSSYYQKVAIFLTKQDNIIKGSIRSNNKINSKTLISIIP
CEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCCCCCCHHHEEECC
SHLVINSGGHKAAAGFTLHENLLEDFIKELEYATTKVEYETTNENESIPIDAILPKNLTK
CCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECCCCCCH
DSLFKTIEIFEPYGYEFREPILLMKNAYLQELKIIDKNHSSKHINMRIKSQNDYYKAIFF
HHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEE
NGTKKIEELDIKEDQYLDIIFTVNEDFYYPREKILKIIDIKKNAQINV
CCCCCHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]