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The map label for this gene is glcB [H]

Identifier: 154248172

GI number: 154248172

Start: 4717931

End: 4720102

Strand: Direct

Name: glcB [H]

Synonym: Xaut_4252

Alternate gene names: 154248172

Gene position: 4717931-4720102 (Clockwise)

Preceding gene: 154248171

Following gene: 154248173

Centisome position: 88.87

GC content: 68.32

Gene sequence:

>2172_bases
ATGGCAAGTGGGACCGAGGCCCGGGTGGAACGCGCCGGGCTGCAAATCGCGCGTACTCTGCACGACTTCATTGAAACCGA
GGCATTGCCCGGCAGCGGCATCGAGGCGGACGTCTTCTGGGAGGGCCTTTCCGCCATCGCCCACGACCTGGCACCGCGCA
ACCGCGAACTCCTGGCGCGGCGCGAGGAGCTGCAGAAGACCCTCGACCAGTGGTATCGCGACAATGGCACCCCCCTCGAT
CTGGAGGCCTACAAGGCCTTCCTGACAAAGATCGGCTATCTGGTGCCCGAAGGCCCCGCCTTCAGCGTGACCACGGCCAA
TGTGGACCCCGAGATCGCCGAAGTGGCCGGCCCGCAGCTGGTCGTGCCGGTGATGAACGCGCGCTACGCGCTGAATGCCG
CCAATGCGCGCTGGGGCTCGCTCTATGACGCGCTCTACGGCACCGACGCCATCCCCGAGACCGACGGCGCCGAGAAGGGC
AAGGGCTACAATCCCGTCCGCGGCGCCAAGGTCATCGCCTGGGGCCGCGCGTTCCTCGACGAGAGCGCGCCCCTGAAGGC
CGGCTCCTGGGCCGATGCCGGTCCGCTGTCGGTCAAGGACGGCGCCCTTGCGGTCGGCGCCACCGGCCTTGCGGACCCGT
CCCAGTTCGCCGGCTATCGGGGCGAGGCCTCGGCGCCGTCCGCCGTGCTGCTGAAGAAGAACGGCCTCCATGTGGAGATC
GTGGTGGACCGCGCCAGCGTCATCGGCAAGGACGATACCGCCGGCATCTCCGACCTGGTGCTGGAAGCCGCCCTCACCAC
CATCCAGGACTGCGAGGATTCCGTCGCCGCCGTGGATGCGGAGGACAAGGTCGCGGTCTACCGCAACTGGCTCGGCCTGA
TGAGCGGCGACCTCAAGGAGGAGATCGCCAAGGGCGGGCGCATCTTCACCCGCAGCCTGAATCCCGACCGGGAATATACC
GCGCCGGACGGCTGCACCCTGGTGCTGCCGGGCCGCTCGCTCATGCTGGTGCGCAACGTGGGCCACCTCATGACCAATCC
GGCCATCCTGCTGAAGGACGGCTCGGAAATCCCCGAGGGCATCATGGATGCCGCCGTCACCGCGCTCATCGCCCTGCGCG
ACATCGGCCCCAACGGACGCCGCGCCAACAGCCGCGCCGGCTCCATGTATGTGGTCAAGCCCAAGATGCACGGGCCGGAA
GAGGTGGCGTTCGCGGTGGAGATCTTCGACCGCGTCGAGGATCTGCTGGGCCTGCCCAGCGACACCATCAAGATGGGCAT
CATGGACGAGGAGCGCCGCACCACGGTGAACCTGAAGGAGAGCATCCGCGCCGCATCCAGCCGGGTTGCCTTCATCAACA
CCGGCTTCCTCGACCGCACCGGCGACGAGATCCACACTTCCATGGAAGCCGGCCCCATGGTCCGCAAGGGCGACATGAAG
CAGGCTGCCTGGATCAGCGCCTACGAGGCCCGCAACGTGGACATCGGCCTGGAGTGCGGCCTTGCCGGCCATGCCCAGAT
CGGCAAGGGCATGTGGGCCATGCCGGACCTGATGGCCGCCATGCTTGAGCAGAAGATCGCCCACCCCAAGGCCGGCGCCA
ACACCGCCTGGGTGCCGTCGCCCACTGCCGCCACCCTGCACGCCACCCACTATCACAAGGTGGACGTGCATCAGGTGCAG
GCCGCGCTGCGCAGCCGCCCGAAGGCCAAGCTGGACGATCTCCTGACCATCCCGGTGGCGGTGCGGCCGAACTGGACGCC
CGAGGACATCCAGCACGAGCTGGACAACAATGCCCAGGGCATCCTCGGCTATGTGGTGCGCTGGGTCGACCAGGGCGTCG
GCTGCTCCAAGGTGCCGGACATCCACGACGTGGGCCTGATGGAGGATCGCGCCACCCTGCGCATCTCCGCCCAGCACATG
GCTAACTGGCTGCGCCACGGCGTGTGCAGCGAGGCGCAGGTGCTGGAGACACTGAAGCGCATGGCGGCGGTGGTGGACCG
GCAGAATGCCGGCGATCCGCTCTACCGGCCCATGGCGCCGGACTTCACCTCCATCGCCTTCCAGGCCGCCTGCGACCTCG
TCTTCAAGGGCCGCGAGCAGCCGAGCGGCTACACCGAGCCGCTGCTCCACCAGCGCCGGCTGGAGCTGAAGGCGCAGAGC
GCCGCGGCCTGA

Upstream 100 bases:

>100_bases
TTGCGGCGTAGAACAAGCGGGCGAGGCTGCTGGCGTCTCAGACGGACCAGCGCTCCTCAGGATGTACCTCGCCCTGGGAA
AAGGACGACACGGAGTGACC

Downstream 100 bases:

>100_bases
CACGCCTCTCACACACGAACCGGAGCGCGGCCGCAGTGTTGCGCTCCGGTAGTGGGCGAGAACCCAGCGCGTCGGCGCGG
GCCACGCCAAAAGGGTCGTT

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MASGTEARVERAGLQIARTLHDFIETEALPGSGIEADVFWEGLSAIAHDLAPRNRELLARREELQKTLDQWYRDNGTPLD
LEAYKAFLTKIGYLVPEGPAFSVTTANVDPEIAEVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETDGAEKG
KGYNPVRGAKVIAWGRAFLDESAPLKAGSWADAGPLSVKDGALAVGATGLADPSQFAGYRGEASAPSAVLLKKNGLHVEI
VVDRASVIGKDDTAGISDLVLEAALTTIQDCEDSVAAVDAEDKVAVYRNWLGLMSGDLKEEIAKGGRIFTRSLNPDREYT
APDGCTLVLPGRSLMLVRNVGHLMTNPAILLKDGSEIPEGIMDAAVTALIALRDIGPNGRRANSRAGSMYVVKPKMHGPE
EVAFAVEIFDRVEDLLGLPSDTIKMGIMDEERRTTVNLKESIRAASSRVAFINTGFLDRTGDEIHTSMEAGPMVRKGDMK
QAAWISAYEARNVDIGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHQVQ
AALRSRPKAKLDDLLTIPVAVRPNWTPEDIQHELDNNAQGILGYVVRWVDQGVGCSKVPDIHDVGLMEDRATLRISAQHM
ANWLRHGVCSEAQVLETLKRMAAVVDRQNAGDPLYRPMAPDFTSIAFQAACDLVFKGREQPSGYTEPLLHQRRLELKAQS
AAA

Sequences:

>Translated_723_residues
MASGTEARVERAGLQIARTLHDFIETEALPGSGIEADVFWEGLSAIAHDLAPRNRELLARREELQKTLDQWYRDNGTPLD
LEAYKAFLTKIGYLVPEGPAFSVTTANVDPEIAEVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETDGAEKG
KGYNPVRGAKVIAWGRAFLDESAPLKAGSWADAGPLSVKDGALAVGATGLADPSQFAGYRGEASAPSAVLLKKNGLHVEI
VVDRASVIGKDDTAGISDLVLEAALTTIQDCEDSVAAVDAEDKVAVYRNWLGLMSGDLKEEIAKGGRIFTRSLNPDREYT
APDGCTLVLPGRSLMLVRNVGHLMTNPAILLKDGSEIPEGIMDAAVTALIALRDIGPNGRRANSRAGSMYVVKPKMHGPE
EVAFAVEIFDRVEDLLGLPSDTIKMGIMDEERRTTVNLKESIRAASSRVAFINTGFLDRTGDEIHTSMEAGPMVRKGDMK
QAAWISAYEARNVDIGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHQVQ
AALRSRPKAKLDDLLTIPVAVRPNWTPEDIQHELDNNAQGILGYVVRWVDQGVGCSKVPDIHDVGLMEDRATLRISAQHM
ANWLRHGVCSEAQVLETLKRMAAVVDRQNAGDPLYRPMAPDFTSIAFQAACDLVFKGREQPSGYTEPLLHQRRLELKAQS
AAA
>Mature_722_residues
ASGTEARVERAGLQIARTLHDFIETEALPGSGIEADVFWEGLSAIAHDLAPRNRELLARREELQKTLDQWYRDNGTPLDL
EAYKAFLTKIGYLVPEGPAFSVTTANVDPEIAEVAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIPETDGAEKGK
GYNPVRGAKVIAWGRAFLDESAPLKAGSWADAGPLSVKDGALAVGATGLADPSQFAGYRGEASAPSAVLLKKNGLHVEIV
VDRASVIGKDDTAGISDLVLEAALTTIQDCEDSVAAVDAEDKVAVYRNWLGLMSGDLKEEIAKGGRIFTRSLNPDREYTA
PDGCTLVLPGRSLMLVRNVGHLMTNPAILLKDGSEIPEGIMDAAVTALIALRDIGPNGRRANSRAGSMYVVKPKMHGPEE
VAFAVEIFDRVEDLLGLPSDTIKMGIMDEERRTTVNLKESIRAASSRVAFINTGFLDRTGDEIHTSMEAGPMVRKGDMKQ
AAWISAYEARNVDIGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPSPTAATLHATHYHKVDVHQVQA
ALRSRPKAKLDDLLTIPVAVRPNWTPEDIQHELDNNAQGILGYVVRWVDQGVGCSKVPDIHDVGLMEDRATLRISAQHMA
NWLRHGVCSEAQVLETLKRMAAVVDRQNAGDPLYRPMAPDFTSIAFQAACDLVFKGREQPSGYTEPLLHQRRLELKAQSA
AA

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=721, Percent_Identity=59.7780859916782, Blast_Score=873, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 78129; Mature: 77997

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTEARVERAGLQIARTLHDFIETEALPGSGIEADVFWEGLSAIAHDLAPRNRELLAR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
REELQKTLDQWYRDNGTPLDLEAYKAFLTKIGYLVPEGPAFSVTTANVDPEIAEVAGPQL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCEE
VVPVMNARYALNAANARWGSLYDALYGTDAIPETDGAEKGKGYNPVRGAKVIAWGRAFLD
EEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHC
ESAPLKAGSWADAGPLSVKDGALAVGATGLADPSQFAGYRGEASAPSAVLLKKNGLHVEI
CCCCCCCCCCCCCCCCEECCCCEEECCCCCCCHHHHCCCCCCCCCCCEEEEECCCCEEEE
VVDRASVIGKDDTAGISDLVLEAALTTIQDCEDSVAAVDAEDKVAVYRNWLGLMSGDLKE
EEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHH
EIAKGGRIFTRSLNPDREYTAPDGCTLVLPGRSLMLVRNVGHLMTNPAILLKDGSEIPEG
HHHCCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCEEEEECCCCCHHH
IMDAAVTALIALRDIGPNGRRANSRAGSMYVVKPKMHGPEEVAFAVEIFDRVEDLLGLPS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
DTIKMGIMDEERRTTVNLKESIRAASSRVAFINTGFLDRTGDEIHTSMEAGPMVRKGDMK
HHEEECCCCCCHHHEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCH
QAAWISAYEARNVDIGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHKVDVHQVQAALRSRPKAKLDDLLTIPVAVRPNWTPEDIQHELDNNAQG
CCCCEEEECEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHH
ILGYVVRWVDQGVGCSKVPDIHDVGLMEDRATLRISAQHMANWLRHGVCSEAQVLETLKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHH
MAAVVDRQNAGDPLYRPMAPDFTSIAFQAACDLVFKGREQPSGYTEPLLHQRRLELKAQS
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AAA
CCC
>Mature Secondary Structure 
ASGTEARVERAGLQIARTLHDFIETEALPGSGIEADVFWEGLSAIAHDLAPRNRELLAR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
REELQKTLDQWYRDNGTPLDLEAYKAFLTKIGYLVPEGPAFSVTTANVDPEIAEVAGPQL
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCEE
VVPVMNARYALNAANARWGSLYDALYGTDAIPETDGAEKGKGYNPVRGAKVIAWGRAFLD
EEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHC
ESAPLKAGSWADAGPLSVKDGALAVGATGLADPSQFAGYRGEASAPSAVLLKKNGLHVEI
CCCCCCCCCCCCCCCCEECCCCEEECCCCCCCHHHHCCCCCCCCCCCEEEEECCCCEEEE
VVDRASVIGKDDTAGISDLVLEAALTTIQDCEDSVAAVDAEDKVAVYRNWLGLMSGDLKE
EEECHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHH
EIAKGGRIFTRSLNPDREYTAPDGCTLVLPGRSLMLVRNVGHLMTNPAILLKDGSEIPEG
HHHCCCEEEEECCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCEEEEECCCCCHHH
IMDAAVTALIALRDIGPNGRRANSRAGSMYVVKPKMHGPEEVAFAVEIFDRVEDLLGLPS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
DTIKMGIMDEERRTTVNLKESIRAASSRVAFINTGFLDRTGDEIHTSMEAGPMVRKGDMK
HHEEECCCCCCHHHEECHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCH
QAAWISAYEARNVDIGLECGLAGHAQIGKGMWAMPDLMAAMLEQKIAHPKAGANTAWVPS
HHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PTAATLHATHYHKVDVHQVQAALRSRPKAKLDDLLTIPVAVRPNWTPEDIQHELDNNAQG
CCCCEEEECEEEECHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHH
ILGYVVRWVDQGVGCSKVPDIHDVGLMEDRATLRISAQHMANWLRHGVCSEAQVLETLKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHH
MAAVVDRQNAGDPLYRPMAPDFTSIAFQAACDLVFKGREQPSGYTEPLLHQRRLELKAQS
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]