Click here to switch to the map view.

The map label for this gene is pcm2 [H]

Identifier: 154248154

GI number: 154248154

Start: 4695007

End: 4695669

Strand: Direct

Name: pcm2 [H]

Synonym: Xaut_4234

Alternate gene names: 154248154

Gene position: 4695007-4695669 (Clockwise)

Preceding gene: 154248153

Following gene: 154248155

Centisome position: 88.44

GC content: 69.68

Gene sequence:

>663_bases
ATGATCGATTTCGTCGAGCTTCGCCGCGGCATGGTGGACGGGCAGGTCCGCACCAACAACGTCACCGATCCGCGCATCGT
CGCCGCCATGCTGGAGATCCCGCGCGAGAAGTTCGTGCCGGCGCCGCTGAAGTCCCTCGCATATATCGACGACGATCTGG
CGCTCACCTCCGGCAATGCCCCGCGCTATCTCATCGAGCCCATGATTCTCGCGCGCCTGGTGCAGCTCGCCGACGTACAG
GAGCATGAGCACGTGCTCGATGTGGGCACCGGCACCGGCTATTCGGCCGCCGTGCTGTCGCGCCTCGCGCAGCAGGTGGT
CGCCGTGGAGGAAGACGCCGGCCTCGCCGCGACCGCGACGGCGACCCTCGCCGAGCTGGGCGTCGGTAACGTGGCGGTGA
TGCAGGGGCCGCTGAACGCCGGCTGGGCCGCCGAGGCGCCCTACGACCTCATCCTGCTCAACGGGGCGGTGGACGAGGTG
CCGGCCAACCTCTTCGCTCAGGTGAAGGAGGGCGGCCGAGTCGTCGCCGTGGTCGGCCATGGGGGCGCCGGACAGGCCCG
CGTGTTCACCAAGGTGGCCGGTGCCATGAGCGAGCGGGTGGCCTTCAACGCCGCCGTGCCCGCGCTGCCCGGCTTCAAGG
CCGCGCCACGCTTCGTTTTCTGA

Upstream 100 bases:

>100_bases
CGATCCGGTCGGCTGAAGGGCGTCTTATCGAGAGTTTTGGCACGGAGCCGCCGCCCGGGATGTTTCGGCCGGGCCGCTCC
GGGTTTGGCCGGAGACGCAT

Downstream 100 bases:

>100_bases
AGCACCTGTGGCGCGTTTGCCGCGCTGACCCGAAAACGTCCGTGACATCGAACCGATCGGTTCCCGATCGCGAGCGGTGC
AGATATCTTTCGAGAAAGTT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIDFVELRRGMVDGQVRTNNVTDPRIVAAMLEIPREKFVPAPLKSLAYIDDDLALTSGNAPRYLIEPMILARLVQLADVQ
EHEHVLDVGTGTGYSAAVLSRLAQQVVAVEEDAGLAATATATLAELGVGNVAVMQGPLNAGWAAEAPYDLILLNGAVDEV
PANLFAQVKEGGRVVAVVGHGGAGQARVFTKVAGAMSERVAFNAAVPALPGFKAAPRFVF

Sequences:

>Translated_220_residues
MIDFVELRRGMVDGQVRTNNVTDPRIVAAMLEIPREKFVPAPLKSLAYIDDDLALTSGNAPRYLIEPMILARLVQLADVQ
EHEHVLDVGTGTGYSAAVLSRLAQQVVAVEEDAGLAATATATLAELGVGNVAVMQGPLNAGWAAEAPYDLILLNGAVDEV
PANLFAQVKEGGRVVAVVGHGGAGQARVFTKVAGAMSERVAFNAAVPALPGFKAAPRFVF
>Mature_220_residues
MIDFVELRRGMVDGQVRTNNVTDPRIVAAMLEIPREKFVPAPLKSLAYIDDDLALTSGNAPRYLIEPMILARLVQLADVQ
EHEHVLDVGTGTGYSAAVLSRLAQQVVAVEEDAGLAATATATLAELGVGNVAVMQGPLNAGWAAEAPYDLILLNGAVDEV
PANLFAQVKEGGRVVAVVGHGGAGQARVFTKVAGAMSERVAFNAAVPALPGFKAAPRFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=165, Percent_Identity=35.7575757575758, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23069; Mature: 23069

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFVELRRGMVDGQVRTNNVTDPRIVAAMLEIPREKFVPAPLKSLAYIDDDLALTSGNA
CCCHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHCCCCEEEECCCC
PRYLIEPMILARLVQLADVQEHEHVLDVGTGTGYSAAVLSRLAQQVVAVEEDAGLAATAT
CHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ATLAELGVGNVAVMQGPLNAGWAAEAPYDLILLNGAVDEVPANLFAQVKEGGRVVAVVGH
HHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEC
GGAGQARVFTKVAGAMSERVAFNAAVPALPGFKAAPRFVF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDFVELRRGMVDGQVRTNNVTDPRIVAAMLEIPREKFVPAPLKSLAYIDDDLALTSGNA
CCCHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCHHHCCCCCHHHHHHCCCCEEEECCCC
PRYLIEPMILARLVQLADVQEHEHVLDVGTGTGYSAAVLSRLAQQVVAVEEDAGLAATAT
CHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ATLAELGVGNVAVMQGPLNAGWAAEAPYDLILLNGAVDEVPANLFAQVKEGGRVVAVVGH
HHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEC
GGAGQARVFTKVAGAMSERVAFNAAVPALPGFKAAPRFVF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA