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The map label for this gene is macA [H]

Identifier: 154248146

GI number: 154248146

Start: 4685481

End: 4686761

Strand: Direct

Name: macA [H]

Synonym: Xaut_4226

Alternate gene names: 154248146

Gene position: 4685481-4686761 (Clockwise)

Preceding gene: 154248145

Following gene: 154248147

Centisome position: 88.26

GC content: 72.29

Gene sequence:

>1281_bases
GTGGACCAGATCACCCCCGCTGCCGACACCGCGTCGCGTGACCTGCCGAAGGCGGCGGGACACGGCGGCCTCATCGCCGC
CGTGTGGCATCGCAAATGGCGGATCGCCGCCCTTATGCTGGTGCTGGCCGGGGCGGGCTGGGGGCTGCTGCGCTGGCGCG
CGGGGCCGGAGGTGGTGGTCTATCCGGTGGTGAAGGCCGAGCTGGTGCGCACCGTGGTCGCCACCGGCCATGTGGAGACC
CCCTACCGGGTGAGCATCGGCAGCCAGATCACCGGCACCGTCAAGGAGGTGCTGGTGGAGGAGGGCCAGAGCGTGCGGCA
GGGCCAGCCGCTGGTTTCCCTCGACGCCACCGAGCTTGAGTCCGCAGTGGTGCAGGCCGAGGGCGCCGTGGCCCAGGCCG
ACGCCCGCATGCGGCAGCTGCGCGAACTCACCAAGCCCGCCGCCGACGAGGCGCAAAAGCAGGCCCGTGCCAACCTCGAC
AATGCCCGCGCCGCCTTCGATCGCGCCGACAAGCTGGCCAAATCTGGCGCCGGCACCCAGGCGACCCTGGACGAGGCGAC
ACGGGCGCTGAATGTCGCCCTGACGCTGGCGCGCACGGCGGAGCTGCAGGTCTACACCTCAAGTCCCGGCGGCAGCGATT
ATGTCATCGCCGAGACCCAGCTGAGCCAGGCCAAGGCGAACCTTGCCACTGCCCGGGCGCGGCTCGGCTACGCCACCATC
ACCGCCCCGCGCGACGGCGTGCTCATCACCCGCAGCGTGGAGCGCGGCGCCGTGGTGCAGGCGGGCAAGACGCTGCTGGT
GCTGGCGCCGGCCGGCGACACCCAGCTCGTGGTCCAGATCGACGAGAAGAACCTCAGCCTGCTCAAGCTCGGCCAGAGCG
CGCTGGCGTCCGCCGACGCCTATCCGGACCAGCGGTTCCCCGCGAGCCTTACCTATATCAACCCCTCCGTGGACATCACC
CGGGCCTCGGTGGAGGTGAAGCTTACGGTGCCCGAACCGCCGGTCTACCTGCGGCAGGACATGACCGTTTCCGTGGACAT
CGCGGTGGATCGCCGGCCGGACGCGGTGGTGGTGCCCGCGCGCACCGTGCACAGCCCCACCTCGGCGGCGCCGTTCGTGC
TGGTGGCGCGGGACGGGCGGGCGCGGGAGCAGAAGGTCCGCCTCGGCCTGAGGGCCGGCGACCAGGTTCAGGTGCTGGAG
GGGCTCACCCCCGGCGAGGCGGTGCTGCCGGTGGCCTCGGGGGTGCGCGCCGGCCAGCGCATCCGGCCGGTGTCGCGATG
A

Upstream 100 bases:

>100_bases
GGACCATGATGCCGGGGTTCGCACCATCGAATCCCGGCGCCCGGCCTTCCCTGATGCGAGCCCCGCGCATGGCGCCAAGC
CGCAACCGTTCAGGGATCAC

Downstream 100 bases:

>100_bases
ACCGCTGGCTGCCGTTCGAATGGATCGCCGCCCTGCGCTTCCTCGGCGAAGGGCGCATGCAGACCGCCTTCATCCTCGGC
GGCATCACCATCGGCGTGGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDQITPAADTASRDLPKAAGHGGLIAAVWHRKWRIAALMLVLAGAGWGLLRWRAGPEVVVYPVVKAELVRTVVATGHVET
PYRVSIGSQITGTVKEVLVEEGQSVRQGQPLVSLDATELESAVVQAEGAVAQADARMRQLRELTKPAADEAQKQARANLD
NARAAFDRADKLAKSGAGTQATLDEATRALNVALTLARTAELQVYTSSPGGSDYVIAETQLSQAKANLATARARLGYATI
TAPRDGVLITRSVERGAVVQAGKTLLVLAPAGDTQLVVQIDEKNLSLLKLGQSALASADAYPDQRFPASLTYINPSVDIT
RASVEVKLTVPEPPVYLRQDMTVSVDIAVDRRPDAVVVPARTVHSPTSAAPFVLVARDGRAREQKVRLGLRAGDQVQVLE
GLTPGEAVLPVASGVRAGQRIRPVSR

Sequences:

>Translated_426_residues
MDQITPAADTASRDLPKAAGHGGLIAAVWHRKWRIAALMLVLAGAGWGLLRWRAGPEVVVYPVVKAELVRTVVATGHVET
PYRVSIGSQITGTVKEVLVEEGQSVRQGQPLVSLDATELESAVVQAEGAVAQADARMRQLRELTKPAADEAQKQARANLD
NARAAFDRADKLAKSGAGTQATLDEATRALNVALTLARTAELQVYTSSPGGSDYVIAETQLSQAKANLATARARLGYATI
TAPRDGVLITRSVERGAVVQAGKTLLVLAPAGDTQLVVQIDEKNLSLLKLGQSALASADAYPDQRFPASLTYINPSVDIT
RASVEVKLTVPEPPVYLRQDMTVSVDIAVDRRPDAVVVPARTVHSPTSAAPFVLVARDGRAREQKVRLGLRAGDQVQVLE
GLTPGEAVLPVASGVRAGQRIRPVSR
>Mature_426_residues
MDQITPAADTASRDLPKAAGHGGLIAAVWHRKWRIAALMLVLAGAGWGLLRWRAGPEVVVYPVVKAELVRTVVATGHVET
PYRVSIGSQITGTVKEVLVEEGQSVRQGQPLVSLDATELESAVVQAEGAVAQADARMRQLRELTKPAADEAQKQARANLD
NARAAFDRADKLAKSGAGTQATLDEATRALNVALTLARTAELQVYTSSPGGSDYVIAETQLSQAKANLATARARLGYATI
TAPRDGVLITRSVERGAVVQAGKTLLVLAPAGDTQLVVQIDEKNLSLLKLGQSALASADAYPDQRFPASLTYINPSVDIT
RASVEVKLTVPEPPVYLRQDMTVSVDIAVDRRPDAVVVPARTVHSPTSAAPFVLVARDGRAREQKVRLGLRAGDQVQVLE
GLTPGEAVLPVASGVRAGQRIRPVSR

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=387, Percent_Identity=26.3565891472868, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789900, Length=306, Percent_Identity=27.4509803921569, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787013, Length=282, Percent_Identity=29.4326241134752, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789929, Length=366, Percent_Identity=24.8633879781421, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45154; Mature: 45154

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQITPAADTASRDLPKAAGHGGLIAAVWHRKWRIAALMLVLAGAGWGLLRWRAGPEVVV
CCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
YPVVKAELVRTVVATGHVETPYRVSIGSQITGTVKEVLVEEGQSVRQGQPLVSLDATELE
EEHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHCCCCEEEECHHHHH
SAVVQAEGAVAQADARMRQLRELTKPAADEAQKQARANLDNARAAFDRADKLAKSGAGTQ
HHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ATLDEATRALNVALTLARTAELQVYTSSPGGSDYVIAETQLSQAKANLATARARLGYATI
HHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHCCEEEE
TAPRDGVLITRSVERGAVVQAGKTLLVLAPAGDTQLVVQIDEKNLSLLKLGQSALASADA
ECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCEEEEEECCCCCEEEHHCHHHHHCCCC
YPDQRFPASLTYINPSVDITRASVEVKLTVPEPPVYLRQDMTVSVDIAVDRRPDAVVVPA
CCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCCEEEECCCEEEEEEEEECCCCEEEECC
RTVHSPTSAAPFVLVARDGRAREQKVRLGLRAGDQVQVLEGLTPGEAVLPVASGVRAGQR
HHCCCCCCCCCEEEEECCCCCCCHHHEEECCCCCEEEEECCCCCCCEEHHHHHCCCCCCC
IRPVSR
CCCCCC
>Mature Secondary Structure
MDQITPAADTASRDLPKAAGHGGLIAAVWHRKWRIAALMLVLAGAGWGLLRWRAGPEVVV
CCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEE
YPVVKAELVRTVVATGHVETPYRVSIGSQITGTVKEVLVEEGQSVRQGQPLVSLDATELE
EEHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHCCCCEEEECHHHHH
SAVVQAEGAVAQADARMRQLRELTKPAADEAQKQARANLDNARAAFDRADKLAKSGAGTQ
HHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ATLDEATRALNVALTLARTAELQVYTSSPGGSDYVIAETQLSQAKANLATARARLGYATI
HHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHCCEEEE
TAPRDGVLITRSVERGAVVQAGKTLLVLAPAGDTQLVVQIDEKNLSLLKLGQSALASADA
ECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCEEEEEECCCCCEEEHHCHHHHHCCCC
YPDQRFPASLTYINPSVDITRASVEVKLTVPEPPVYLRQDMTVSVDIAVDRRPDAVVVPA
CCCCCCCCEEEEECCCCEEEEEEEEEEEECCCCCEEEECCCEEEEEEEEECCCCEEEECC
RTVHSPTSAAPFVLVARDGRAREQKVRLGLRAGDQVQVLEGLTPGEAVLPVASGVRAGQR
HHCCCCCCCCCEEEEECCCCCCCHHHEEECCCCCEEEEECCCCCCCEEHHHHHCCCCCCC
IRPVSR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]