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The map label for this gene is oprM [H]

Identifier: 154248089

GI number: 154248089

Start: 4615790

End: 4617202

Strand: Direct

Name: oprM [H]

Synonym: Xaut_4169

Alternate gene names: 154248089

Gene position: 4615790-4617202 (Clockwise)

Preceding gene: 154248088

Following gene: 154248093

Centisome position: 86.94

GC content: 69.0

Gene sequence:

>1413_bases
ATGAGGTTTTTCAGCACATTTGGGCGGCGCCCGCGTGGGGCGCTTCTGGCCGCCGGCGCCCTCGCGCTGCTGCTCGCCGG
CTGCCTGCCGCAGGAGCCGGCGCCGGACGTGGGGATCGCCACCCCCACCCATTATGTGGAAGCGCCGGCCAACCCAAACG
CCCATCTCCCGCCGCCCGAATGGTGGCGGAGCTTTCGCTCCAGCGACCTCACCCGCTTCGTGGCGGAAGCCAACGTCGAC
AATCTCGACATCGCCGTCGCCACCGCTGAAATCGTGCAGGCCGACGCCCAGGTGGGCATATCCGGCGCACCGCTCTACCC
CAACCTCACCTTCGCGGGCTCTGCCGCGCAGATCCGCTCCAGCGGCAGCAGCGGCAATCCGGGTAACACCAGCGGCCAGT
ATATTCTCGGCCTGTCCGCCGGCTACATGGTGGACTTCTGGGGAAAGAACCGCTCCGGCCTTCTGGCGGCGGAAGAGAAT
GCGGCGGCGGCCCGCTTCAATCGCGATGTGGTGACGCTGTCCACCCAGGTGGCGGTGGCCAATACCTATTTCCAGATCCT
CGGCGCCCGTGACCAGCTGCGCATCGCGCAGAACAGCCTGGCCGCCGCAACCCGCATCCTCGACATCGTGAAGCGACAGT
TCGCCGGCGGCACCGTCTCGCAGCTCGACGTGTCGCAGCAGGAGGCGCTGGTGGCGCAGGTGCGGGCCTCGATCCCGCCC
ATCGAGATCACCCTGCGCCAGAACTGGGCGGCGCTGGCCGTGCTGCTGGCGCGCACGCCCGTCGCGCTCACCCCGCCGCA
GGGGAGCCTGTCGCAGATCACCGTGCCGCGCATCACCCCGGGATTACCGTCTGACCTCCTCACCCGCCGGCCCGATATTG
CCCTCGCCGAGGCCCAGCTCCAGGCAGGGGGCTATAATGTGGAGGCGGCGCGGGCGGCCATGCTGCCGCAGATCCAGCTG
ACCGGCACCGGGGGAGTGCAGAGCGCGGCGCTTCAGGCGCTGTTCGCCCCTGGGGCTTGGTACTACACGCTGGTTGCGGG
GCTGACCCAGCCCATTTTCGATGGCTACCTGCTGCAGAGCCAGCTCGACCAGGCCAAGGGCGTGCAGGTGCAGGACCTGC
AGGTCTATCGCAAGGCCATCCTGTCGGCCTTCGCCGATGTGGAGAAGGCGCTGGTGGCGCTGGACCAGACCAACAAGCAG
GTGAAGCTGCAGGCCGACGTGGTGGAAAGCTCACGCACCGCGTTCCGCGTGGCCGAATCCCAGCTGGCGGGCGGCACCAC
CACCCTCGTCGCCGTGCTTCAGGCGCAGCAGACCTACTTTACAGCCGAGAATGGCCTGGCGCAGGCGCGGCTGTCGCGGC
TGCTGGCCGCCACCAGCCTGTTCCAGGCGCTGGGTGGCGGGTGGGGCCCCTGA

Upstream 100 bases:

>100_bases
GACGCCGGTGGTCTATCTCTACATCGACCGTCTGCGCCGGGGGCGCCTCCACACGGACCGGCGTGCCCGCCTGCGGCCGC
CGCCAGAGCCGGCCGCATAG

Downstream 100 bases:

>100_bases
GGGTTATGGCTGCACCGCGGCCACCGCATCGTCCACGCTGTAGCTGCAGCGCGCCACCAGATCGCGGGCGCCGGCGGCCG
CCAGCAGGTCGCGGACACGA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRFFSTFGRRPRGALLAAGALALLLAGCLPQEPAPDVGIATPTHYVEAPANPNAHLPPPEWWRSFRSSDLTRFVAEANVD
NLDIAVATAEIVQADAQVGISGAPLYPNLTFAGSAAQIRSSGSSGNPGNTSGQYILGLSAGYMVDFWGKNRSGLLAAEEN
AAAARFNRDVVTLSTQVAVANTYFQILGARDQLRIAQNSLAAATRILDIVKRQFAGGTVSQLDVSQQEALVAQVRASIPP
IEITLRQNWAALAVLLARTPVALTPPQGSLSQITVPRITPGLPSDLLTRRPDIALAEAQLQAGGYNVEAARAAMLPQIQL
TGTGGVQSAALQALFAPGAWYYTLVAGLTQPIFDGYLLQSQLDQAKGVQVQDLQVYRKAILSAFADVEKALVALDQTNKQ
VKLQADVVESSRTAFRVAESQLAGGTTTLVAVLQAQQTYFTAENGLAQARLSRLLAATSLFQALGGGWGP

Sequences:

>Translated_470_residues
MRFFSTFGRRPRGALLAAGALALLLAGCLPQEPAPDVGIATPTHYVEAPANPNAHLPPPEWWRSFRSSDLTRFVAEANVD
NLDIAVATAEIVQADAQVGISGAPLYPNLTFAGSAAQIRSSGSSGNPGNTSGQYILGLSAGYMVDFWGKNRSGLLAAEEN
AAAARFNRDVVTLSTQVAVANTYFQILGARDQLRIAQNSLAAATRILDIVKRQFAGGTVSQLDVSQQEALVAQVRASIPP
IEITLRQNWAALAVLLARTPVALTPPQGSLSQITVPRITPGLPSDLLTRRPDIALAEAQLQAGGYNVEAARAAMLPQIQL
TGTGGVQSAALQALFAPGAWYYTLVAGLTQPIFDGYLLQSQLDQAKGVQVQDLQVYRKAILSAFADVEKALVALDQTNKQ
VKLQADVVESSRTAFRVAESQLAGGTTTLVAVLQAQQTYFTAENGLAQARLSRLLAATSLFQALGGGWGP
>Mature_470_residues
MRFFSTFGRRPRGALLAAGALALLLAGCLPQEPAPDVGIATPTHYVEAPANPNAHLPPPEWWRSFRSSDLTRFVAEANVD
NLDIAVATAEIVQADAQVGISGAPLYPNLTFAGSAAQIRSSGSSGNPGNTSGQYILGLSAGYMVDFWGKNRSGLLAAEEN
AAAARFNRDVVTLSTQVAVANTYFQILGARDQLRIAQNSLAAATRILDIVKRQFAGGTVSQLDVSQQEALVAQVRASIPP
IEITLRQNWAALAVLLARTPVALTPPQGSLSQITVPRITPGLPSDLLTRRPDIALAEAQLQAGGYNVEAARAAMLPQIQL
TGTGGVQSAALQALFAPGAWYYTLVAGLTQPIFDGYLLQSQLDQAKGVQVQDLQVYRKAILSAFADVEKALVALDQTNKQ
VKLQADVVESSRTAFRVAESQLAGGTTTLVAVLQAQQTYFTAENGLAQARLSRLLAATSLFQALGGGWGP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=30.1724137931034, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI2367348, Length=352, Percent_Identity=25, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49640; Mature: 49640

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFSTFGRRPRGALLAAGALALLLAGCLPQEPAPDVGIATPTHYVEAPANPNAHLPPPE
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHH
WWRSFRSSDLTRFVAEANVDNLDIAVATAEIVQADAQVGISGAPLYPNLTFAGSAAQIRS
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHC
SGSSGNPGNTSGQYILGLSAGYMVDFWGKNRSGLLAAEENAAAARFNRDVVTLSTQVAVA
CCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEEECCCCHHHHCCCCEEEEEEHHHHH
NTYFQILGARDQLRIAQNSLAAATRILDIVKRQFAGGTVSQLDVSQQEALVAQVRASIPP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCC
IEITLRQNWAALAVLLARTPVALTPPQGSLSQITVPRITPGLPSDLLTRRPDIALAEAQL
EEEEECCCHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCHHHHHCCCCCEEEHHHH
QAGGYNVEAARAAMLPQIQLTGTGGVQSAALQALFAPGAWYYTLVAGLTQPIFDGYLLQS
HCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QLDQAKGVQVQDLQVYRKAILSAFADVEKALVALDQTNKQVKLQADVVESSRTAFRVAES
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHH
QLAGGTTTLVAVLQAQQTYFTAENGLAQARLSRLLAATSLFQALGGGWGP
HHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRFFSTFGRRPRGALLAAGALALLLAGCLPQEPAPDVGIATPTHYVEAPANPNAHLPPPE
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHH
WWRSFRSSDLTRFVAEANVDNLDIAVATAEIVQADAQVGISGAPLYPNLTFAGSAAQIRS
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHC
SGSSGNPGNTSGQYILGLSAGYMVDFWGKNRSGLLAAEENAAAARFNRDVVTLSTQVAVA
CCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCEEEECCCCHHHHCCCCEEEEEEHHHHH
NTYFQILGARDQLRIAQNSLAAATRILDIVKRQFAGGTVSQLDVSQQEALVAQVRASIPP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCC
IEITLRQNWAALAVLLARTPVALTPPQGSLSQITVPRITPGLPSDLLTRRPDIALAEAQL
EEEEECCCHHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCHHHHHCCCCCEEEHHHH
QAGGYNVEAARAAMLPQIQLTGTGGVQSAALQALFAPGAWYYTLVAGLTQPIFDGYLLQS
HCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QLDQAKGVQVQDLQVYRKAILSAFADVEKALVALDQTNKQVKLQADVVESSRTAFRVAES
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHH
QLAGGTTTLVAVLQAQQTYFTAENGLAQARLSRLLAATSLFQALGGGWGP
HHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]