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The map label for this gene is folD [H]

Identifier: 154248007

GI number: 154248007

Start: 4525952

End: 4526839

Strand: Direct

Name: folD [H]

Synonym: Xaut_4086

Alternate gene names: 154248007

Gene position: 4525952-4526839 (Clockwise)

Preceding gene: 154248006

Following gene: 154248015

Centisome position: 85.25

GC content: 69.37

Gene sequence:

>888_bases
ATGGGGGATGTGGTGATCGAGACCAGGCCCATCGACGGAAAGGCGTTCGCAGCCGGGCTCCGGGCCCGCATTGCCGAGGA
GGTGGCCCGCCTCGGCGCCGCCCACGGGCTCAAGCCGGGCCTTGCCGTGGTCCTCGTGGGCGAGGACCCGGCCAGCCAGG
TTTACGTGCGCAACAAGGCGGCCCAGACCCTAGAGGCCGGCATGACCTCCTTCGAGCACAAGCTCGCGGTGGACACGCCC
GAGACCGAGCTGCTGGCGCTGATCGCCCGACTCAACGCCGATCCGGCAGTGAACGGCATTCTGGTTCAGCTGCCGCTGCC
GGCGCAGATCGATGCCATGAAGGTGCTCTCCACCATCGATCCGGCCAAGGACGTGGACGGCTTCCATGTGGTGAATGCCG
GCCGCCTCGCTGTGGGCCTCGACGCGCTGGTGCCGTGCACGCCGCTCGGCTGCGTGATGCTGCTGAAGCACCACCTGGGC
AACCTTTCCGGCCTCAAGGCAGTGGTGGTGGGGCGCTCCAACATTGTCGGCAAGCCGGCGGCGCAGCTGCTGCTGAAAGA
GGACTGCACCGTCACCATCGCCCATTCGCGCACCCGCGACCTGCCCGGCGAGTGCCGCACCGCCGACATCCTGGTCGCCG
CCGTGGGCCGGCCGGAGATGGTGCGCGGCGACTGGATCAAGCCCGGCGCCACCGTGATCGACGTGGGCATCAACCGCATT
CCCAAGCCCGACGGCAAGACGCGCCTCGTGGGCGACGTAGCCTATCAGGAGGCGCAGGGCATCGCCGGCCTGATCACCCC
GGTTCCGGGCGGCGTCGGCCCCATGACCATCGCCTGCCTGCTCCAGAACACCCTCACCGCCGCCAAGCGGCAGGCGGGTC
TGGAATAG

Upstream 100 bases:

>100_bases
GAGCATCAGGCCCGCGCGCGGCACCGCCCTTCCCTGGATGAGGCGCGCCGTGGCCGGCGGGCAGACGGCCCGTCCACCCT
TGCCGACGGGACCGAGGCAT

Downstream 100 bases:

>100_bases
CGCCGGGCCGCTCAGCCCGGCGCAGCCGGCTGGGGGCCTGGAATAACAAAGGGCCGGTGAGCTTGGCGCACCGGCCCTTT
GACTTATCTGGAATCCGACT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGDVVIETRPIDGKAFAAGLRARIAEEVARLGAAHGLKPGLAVVLVGEDPASQVYVRNKAAQTLEAGMTSFEHKLAVDTP
ETELLALIARLNADPAVNGILVQLPLPAQIDAMKVLSTIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLG
NLSGLKAVVVGRSNIVGKPAAQLLLKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRI
PKPDGKTRLVGDVAYQEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQAGLE

Sequences:

>Translated_295_residues
MGDVVIETRPIDGKAFAAGLRARIAEEVARLGAAHGLKPGLAVVLVGEDPASQVYVRNKAAQTLEAGMTSFEHKLAVDTP
ETELLALIARLNADPAVNGILVQLPLPAQIDAMKVLSTIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLG
NLSGLKAVVVGRSNIVGKPAAQLLLKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRI
PKPDGKTRLVGDVAYQEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQAGLE
>Mature_294_residues
GDVVIETRPIDGKAFAAGLRARIAEEVARLGAAHGLKPGLAVVLVGEDPASQVYVRNKAAQTLEAGMTSFEHKLAVDTPE
TELLALIARLNADPAVNGILVQLPLPAQIDAMKVLSTIDPAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGN
LSGLKAVVVGRSNIVGKPAAQLLLKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRIP
KPDGKTRLVGDVAYQEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQAGLE

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=47.9166666666667, Blast_Score=245, Evalue=3e-65,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=43.298969072165, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=43.2432432432432, Blast_Score=204, Evalue=6e-53,
Organism=Homo sapiens, GI36796743, Length=201, Percent_Identity=31.8407960199005, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=53.405017921147, Blast_Score=260, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=42.9065743944637, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=44.0559440559441, Blast_Score=238, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=45.578231292517, Blast_Score=237, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=322, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=50, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=50, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=47.5694444444444, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=47.5694444444444, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=47.5694444444444, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=47.5694444444444, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30719; Mature: 30588

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDVVIETRPIDGKAFAAGLRARIAEEVARLGAAHGLKPGLAVVLVGEDPASQVYVRNKA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHH
AQTLEAGMTSFEHKLAVDTPETELLALIARLNADPAVNGILVQLPLPAQIDAMKVLSTID
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCC
PAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLKAVVVGRSNIVGKPA
CCCCCCCEEEEECCCEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHH
AQLLLKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRI
HHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCEEEEECCCCC
PKPDGKTRLVGDVAYQEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQAGLE
CCCCCCEEEEHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GDVVIETRPIDGKAFAAGLRARIAEEVARLGAAHGLKPGLAVVLVGEDPASQVYVRNKA
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHH
AQTLEAGMTSFEHKLAVDTPETELLALIARLNADPAVNGILVQLPLPAQIDAMKVLSTID
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCC
PAKDVDGFHVVNAGRLAVGLDALVPCTPLGCVMLLKHHLGNLSGLKAVVVGRSNIVGKPA
CCCCCCCEEEEECCCEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHH
AQLLLKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRI
HHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCEEEEECCCCC
PKPDGKTRLVGDVAYQEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQAGLE
CCCCCCEEEEHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA