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The map label for this gene is dctB [H]

Identifier: 154247858

GI number: 154247858

Start: 4355265

End: 4357268

Strand: Direct

Name: dctB [H]

Synonym: Xaut_3935

Alternate gene names: 154247858

Gene position: 4355265-4357268 (Clockwise)

Preceding gene: 154247854

Following gene: 154247859

Centisome position: 82.04

GC content: 65.02

Gene sequence:

>2004_bases
GTGGTCATGGCAACAGCAACCATTGGAAAGGTGTCGGGCGCTGATGCCGGCGCCCGTACCATTCCCTGCCGGTATGCGGG
GCGGTGTGGCGGTGCATGCCGGGAATTTCGCGGGGACGTGCTGGACCCCGCCATCTTCGCCGAGCTTGACTGCGTGGCCT
ATCATCGCCGGCGCGGCGTCCTGGTGTTTCTGCTGGTGGCCTTTGCCGCCGCGGCCGTCGGTTTCTGGCTCGCCTTCACC
TTCGACCAGCGGCAGGGCCTGGCCGAGCTGAAGACGTTCGGCGACGAGCAACTGCACCGCTGGAGCGCGCGGCTGGAGCG
GGAGATCGACAAGTTCGGCATCCTGCCGCTGACCGTGGCCATGGACCGGGATGTGATCTCCTTCCTCACCGCGCCCGACC
AAAGCGAAAAGCGGGACGCCTTGAGCGCCTATCTCGCCCGCCTGAACATCGCCGCCGGCACGCAGCAGGTCTTCGTCATC
GACACCAAGGCAGAGGTGGTCGCATCGAGCAACTGGCAGGATGCCAAGAGCTTCGTCGGCCGTAACATCTCCTATCGGCC
CTATTACCGGGATGCGGTCCCGGGCAGGGTGACCGGCTATTACGCCATCGGCACCACCGGCAACGCGCCCGGATACTATC
TTGCGACGGAAGTCGAGGAGAACGGCCGGCGCGTCGGCGTGGTGGCCGTGAAGATCGACCTGGAACAACTTGAGTCCGAC
TGGCTGAACGGCACCGCCGAGCCCATCCTCATGACCGATGCCAATGACATCGTGGTCCTGTCCTCCCGCGAGGACTGGAA
GTATCGCAGCCTCGGTCCTCTCGATCCCAAGAAGATCGAGCAGATGAACGAGACCCAGCAATACAATCGCCACATCGTGG
AGCCGTTGGTCTGGCAGGCTACCGGGGGCGCGGGCGACGGCCGCAGCTTCATTATCACCGGTGACCGCAGCGGCACGCGC
ACCTATCTCGCCGACAGCCGCTACCTGCCGAATGTCGCCATGACCCTGACCGTGCTTTCCGACACCACGGGCGTGAGCGT
CCGGGCCGCCGAACGGGCGACGGTGGCCGCCGTTCTCATCCTGCTGGTGGCGCTGTCGGTGCGCATCGTCAACCAGCGCC
GGCTCGCCACCCGGGAGCGGCTGCTCGCCCGCGATGCGCTGCAGGAGGCCTATAATTATTTGGAGCGCCAGTTCGAGGAG
CGCAATCGGCAGCTTCGGTCCACCAATGAAGAGCTGCGCCACGAAGTGGTCGAACGCATCAAGGCAGCGCGCCGGCTCAA
GTCCTTCCAGGACGAAATGATCCGCACCGAGAACCTGGCGGTGATCGGCCAGCTTTCGGCCGGCCTTGCCCATGAAATCA
ACCAGCCCCTCGCAGCATTGTCCACCCTGTCGGAAAACGCGGTGCGGTTTCTGGAGCTGAACGACACCAGCACGGTGCGC
CACAATCTGGAACGCATCTGCGATCTGGTCCGGCGCATGGGCGTGCTCACCGGGCAGTTGCGCTCCTTTGCCCGGCGCAC
CGACGGCGAATTGGGGCCGGTGGATCTTCACCATTCCATCGACAGTGCGGTGGCGCTGCTCGGCCACCGCATCAAGAAGG
AAAAGGTGGATCTCGTCGTCCACAAGCCACAACAGCCGATCACGGTGATGGGCGATGCCGTGCGCCTGGAACAGGTGCTG
GTGAACCTCATCAGCAATGCCATGGACGCGCTCACCGGCAATGGCGAGCCGCGGATCGAGATCACCGCGGGCCGCGACGG
CGCCTGGGCGGTGATCGACGTGGTCGACAATGGGCACGGCCTGAGCCACACCGTGATGGACCGATTGTTCGAGCCCTTCT
TCACCACCAAGAAGACCAGCGGGCTGGGGCTCGGCCTTGCCATTTCGCAGGATATCGTGCGCCGCTTCGGTGGCGACCTC
ACCGCCGCCAACACCGCCGCGGGCGGCGCTCGTTTCCGCGTCAGCCTGCGGCCTGTCGAAGCCTCGGAGAACGATCATGG
TTGA

Upstream 100 bases:

>100_bases
TGCGGAGCAGATGTTCGGAAATCCGGCCGCGCTGATCCAGATCATGGAGCGCTGCACGCGTCAGTTTAAACTTTCGTCGT
AAAGGAAGGACGGCTCGGCC

Downstream 100 bases:

>100_bases
TGCCGACGCCATCGTCATCGAGGACGATGAGGACGTGAAGCTCGGCTGCCTGCAGGCGCTGGCGCTGGAGGGCATCAAGG
CCATCGGCTACGACAGCGTC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MVMATATIGKVSGADAGARTIPCRYAGRCGGACREFRGDVLDPAIFAELDCVAYHRRRGVLVFLLVAFAAAAVGFWLAFT
FDQRQGLAELKTFGDEQLHRWSARLEREIDKFGILPLTVAMDRDVISFLTAPDQSEKRDALSAYLARLNIAAGTQQVFVI
DTKAEVVASSNWQDAKSFVGRNISYRPYYRDAVPGRVTGYYAIGTTGNAPGYYLATEVEENGRRVGVVAVKIDLEQLESD
WLNGTAEPILMTDANDIVVLSSREDWKYRSLGPLDPKKIEQMNETQQYNRHIVEPLVWQATGGAGDGRSFIITGDRSGTR
TYLADSRYLPNVAMTLTVLSDTTGVSVRAAERATVAAVLILLVALSVRIVNQRRLATRERLLARDALQEAYNYLERQFEE
RNRQLRSTNEELRHEVVERIKAARRLKSFQDEMIRTENLAVIGQLSAGLAHEINQPLAALSTLSENAVRFLELNDTSTVR
HNLERICDLVRRMGVLTGQLRSFARRTDGELGPVDLHHSIDSAVALLGHRIKKEKVDLVVHKPQQPITVMGDAVRLEQVL
VNLISNAMDALTGNGEPRIEITAGRDGAWAVIDVVDNGHGLSHTVMDRLFEPFFTTKKTSGLGLGLAISQDIVRRFGGDL
TAANTAAGGARFRVSLRPVEASENDHG

Sequences:

>Translated_667_residues
MVMATATIGKVSGADAGARTIPCRYAGRCGGACREFRGDVLDPAIFAELDCVAYHRRRGVLVFLLVAFAAAAVGFWLAFT
FDQRQGLAELKTFGDEQLHRWSARLEREIDKFGILPLTVAMDRDVISFLTAPDQSEKRDALSAYLARLNIAAGTQQVFVI
DTKAEVVASSNWQDAKSFVGRNISYRPYYRDAVPGRVTGYYAIGTTGNAPGYYLATEVEENGRRVGVVAVKIDLEQLESD
WLNGTAEPILMTDANDIVVLSSREDWKYRSLGPLDPKKIEQMNETQQYNRHIVEPLVWQATGGAGDGRSFIITGDRSGTR
TYLADSRYLPNVAMTLTVLSDTTGVSVRAAERATVAAVLILLVALSVRIVNQRRLATRERLLARDALQEAYNYLERQFEE
RNRQLRSTNEELRHEVVERIKAARRLKSFQDEMIRTENLAVIGQLSAGLAHEINQPLAALSTLSENAVRFLELNDTSTVR
HNLERICDLVRRMGVLTGQLRSFARRTDGELGPVDLHHSIDSAVALLGHRIKKEKVDLVVHKPQQPITVMGDAVRLEQVL
VNLISNAMDALTGNGEPRIEITAGRDGAWAVIDVVDNGHGLSHTVMDRLFEPFFTTKKTSGLGLGLAISQDIVRRFGGDL
TAANTAAGGARFRVSLRPVEASENDHG
>Mature_667_residues
MVMATATIGKVSGADAGARTIPCRYAGRCGGACREFRGDVLDPAIFAELDCVAYHRRRGVLVFLLVAFAAAAVGFWLAFT
FDQRQGLAELKTFGDEQLHRWSARLEREIDKFGILPLTVAMDRDVISFLTAPDQSEKRDALSAYLARLNIAAGTQQVFVI
DTKAEVVASSNWQDAKSFVGRNISYRPYYRDAVPGRVTGYYAIGTTGNAPGYYLATEVEENGRRVGVVAVKIDLEQLESD
WLNGTAEPILMTDANDIVVLSSREDWKYRSLGPLDPKKIEQMNETQQYNRHIVEPLVWQATGGAGDGRSFIITGDRSGTR
TYLADSRYLPNVAMTLTVLSDTTGVSVRAAERATVAAVLILLVALSVRIVNQRRLATRERLLARDALQEAYNYLERQFEE
RNRQLRSTNEELRHEVVERIKAARRLKSFQDEMIRTENLAVIGQLSAGLAHEINQPLAALSTLSENAVRFLELNDTSTVR
HNLERICDLVRRMGVLTGQLRSFARRTDGELGPVDLHHSIDSAVALLGHRIKKEKVDLVVHKPQQPITVMGDAVRLEQVL
VNLISNAMDALTGNGEPRIEITAGRDGAWAVIDVVDNGHGLSHTVMDRLFEPFFTTKKTSGLGLGLAISQDIVRRFGGDL
TAANTAAGGARFRVSLRPVEASENDHG

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=32.5102880658436, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=30.5439330543933, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081816, Length=304, Percent_Identity=25.6578947368421, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=279, Percent_Identity=25.4480286738351, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=29.2035398230088, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=32.3008849557522, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=241, Percent_Identity=28.2157676348548, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73629; Mature: 73629

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMATATIGKVSGADAGARTIPCRYAGRCGGACREFRGDVLDPAIFAELDCVAYHRRRGV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
LVFLLVAFAAAAVGFWLAFTFDQRQGLAELKTFGDEQLHRWSARLEREIDKFGILPLTVA
HHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEE
MDRDVISFLTAPDQSEKRDALSAYLARLNIAAGTQQVFVIDTKAEVVASSNWQDAKSFVG
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHEECCCCHHHHHHHC
RNISYRPYYRDAVPGRVTGYYAIGTTGNAPGYYLATEVEENGRRVGVVAVKIDLEQLESD
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEEHHHHHHH
WLNGTAEPILMTDANDIVVLSSREDWKYRSLGPLDPKKIEQMNETQQYNRHIVEPLVWQA
HCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
TGGAGDGRSFIITGDRSGTRTYLADSRYLPNVAMTLTVLSDTTGVSVRAAERATVAAVLI
CCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHH
LLVALSVRIVNQRRLATRERLLARDALQEAYNYLERQFEERNRQLRSTNEELRHEVVERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KAARRLKSFQDEMIRTENLAVIGQLSAGLAHEINQPLAALSTLSENAVRFLELNDTSTVR
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCHHHH
HNLERICDLVRRMGVLTGQLRSFARRTDGELGPVDLHHSIDSAVALLGHRIKKEKVDLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
HKPQQPITVMGDAVRLEQVLVNLISNAMDALTGNGEPRIEITAGRDGAWAVIDVVDNGHG
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCC
LSHTVMDRLFEPFFTTKKTSGLGLGLAISQDIVRRFGGDLTAANTAAGGARFRVSLRPVE
CHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
ASENDHG
CCCCCCC
>Mature Secondary Structure
MVMATATIGKVSGADAGARTIPCRYAGRCGGACREFRGDVLDPAIFAELDCVAYHRRRGV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
LVFLLVAFAAAAVGFWLAFTFDQRQGLAELKTFGDEQLHRWSARLEREIDKFGILPLTVA
HHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEE
MDRDVISFLTAPDQSEKRDALSAYLARLNIAAGTQQVFVIDTKAEVVASSNWQDAKSFVG
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHEECCCCHHHHHHHC
RNISYRPYYRDAVPGRVTGYYAIGTTGNAPGYYLATEVEENGRRVGVVAVKIDLEQLESD
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEEHHHHHHH
WLNGTAEPILMTDANDIVVLSSREDWKYRSLGPLDPKKIEQMNETQQYNRHIVEPLVWQA
HCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
TGGAGDGRSFIITGDRSGTRTYLADSRYLPNVAMTLTVLSDTTGVSVRAAERATVAAVLI
CCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHH
LLVALSVRIVNQRRLATRERLLARDALQEAYNYLERQFEERNRQLRSTNEELRHEVVERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
KAARRLKSFQDEMIRTENLAVIGQLSAGLAHEINQPLAALSTLSENAVRFLELNDTSTVR
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCHHHH
HNLERICDLVRRMGVLTGQLRSFARRTDGELGPVDLHHSIDSAVALLGHRIKKEKVDLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
HKPQQPITVMGDAVRLEQVLVNLISNAMDALTGNGEPRIEITAGRDGAWAVIDVVDNGHG
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCC
LSHTVMDRLFEPFFTTKKTSGLGLGLAISQDIVRRFGGDLTAANTAAGGARFRVSLRPVE
CHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEEEEC
ASENDHG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]