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The map label for this gene is livJ [C]

Identifier: 154247828

GI number: 154247828

Start: 4322113

End: 4323279

Strand: Direct

Name: livJ [C]

Synonym: Xaut_3905

Alternate gene names: 154247828

Gene position: 4322113-4323279 (Clockwise)

Preceding gene: 154247827

Following gene: 154247829

Centisome position: 81.41

GC content: 64.35

Gene sequence:

>1167_bases
ATGCGCCGCGGAGTTTCACTGGGAGGCGGACCGGTGTTCCGCTCCCGGACGAAAGGGAGAGACCTGATGAAGAAGCTCTT
GATGGCGTCGCTCGCTCTGGGCGCCAGCCTTCTGCTTGCGGCTGAGGCCCAGGCGCAGGTGAAGATGGGCGTCGGCGGTC
CCATGACCGGCGCGAATGCTGCCTTCGGCGCCCAGCTGAAGACCGGCGCCGAGCAGGCCGTGGCCGACATCAACGCCGCC
GGCGGCATCCTCGGCCAGAAGATCCAGCTCTCGGTTGGCGACGACGGCGGCAAGCCCGAGCAGGGCAAGTCTGCGGCCAA
CAAGTTCATCTCCGACGGCGTCAAGTTCGTGGTCGGCCACTTCAACTCCGGCGTGTCGATCCCGACTTCGCAGGATTACG
AGGAAGCCGGCGTCCTCCAGATCAGCCCCGCCTCCACCAACCCGACCTTCACCGAGCGCAAGATGTGGAACACGTTCCGC
ACCTGCGGCCGGGACGACCAGCAGGGCGCGGTGGCCGGCGCCTATATCGTCAAGAACTTCAAGGGCAAGAAGGTCGCCAT
CGTCCACGACAAGACCCCCTACGGCAAGGGTCTGGCGGACGAGACGCAGAAGACCATCAACAAGGGCGGCGTGAAGGAAG
TCCTCTATGAGGGCATCAATCCCGGCGAGAAGGACTATTCGGCCCTCGTCTCCAAGCTGAAGGCCAATGGCGTCGACCTG
CTCTACTACGGCGGCCTGCATCCCGAGGCCGGCCTTCTGGTACGTCAGATGCGCGACCAGGGCATGAAGACTGTGCTGTT
CTCTGGCGACGGCATCACCGACAAGGAATACTGGACCATCGCCGGTCCCGGCGCCGAGGGCACGCTGATGACCTTCGGCC
CCGATCCGCGCAACAATCCGGCGGCCAAGGACATCGTCGCCGCCTTCAAGGCCAAGGGCGTGGATCCGGAAGGTTACGTG
CTCTACTCCTATGCCGCGGTGCAGGTGATGAAGCAGGCGGCCGAGGCGGCCAAGTCCCTCGATCCCAAGAAGGTGGCCGC
CGAGATCCATTCCGGCAAGACCTTCAACACCGTGCTCGGCCCGCTCTCCTTCGACAAGAAGGGCGACATCACCAAGCTCG
ACTATGTGGTCTACGTCTGGAAGGACGGCGCCTACACCCAGCTCTGA

Upstream 100 bases:

>100_bases
ACGAGCCGGCGGGCAAGCTCGCCTGGCGTCCAAAGACATTCGGTTGAAACCGGCGCGAGGTGCCGCGCGCGGCATCGACA
GGCCGGGGAATTGCGGCACA

Downstream 100 bases:

>100_bases
CCGGTCGGAGCGTTTTCGAGCGAAGTGAACACCGGTTCGCATGACGGAAACGCACCAGTCTCGGTGACAGTAAGGCCCGC
GCTCTGCGCGGGCCTTTTTG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRRGVSLGGGPVFRSRTKGRDLMKKLLMASLALGASLLLAAEAQAQVKMGVGGPMTGANAAFGAQLKTGAEQAVADINAA
GGILGQKIQLSVGDDGGKPEQGKSAANKFISDGVKFVVGHFNSGVSIPTSQDYEEAGVLQISPASTNPTFTERKMWNTFR
TCGRDDQQGAVAGAYIVKNFKGKKVAIVHDKTPYGKGLADETQKTINKGGVKEVLYEGINPGEKDYSALVSKLKANGVDL
LYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRNNPAAKDIVAAFKAKGVDPEGYV
LYSYAAVQVMKQAAEAAKSLDPKKVAAEIHSGKTFNTVLGPLSFDKKGDITKLDYVVYVWKDGAYTQL

Sequences:

>Translated_388_residues
MRRGVSLGGGPVFRSRTKGRDLMKKLLMASLALGASLLLAAEAQAQVKMGVGGPMTGANAAFGAQLKTGAEQAVADINAA
GGILGQKIQLSVGDDGGKPEQGKSAANKFISDGVKFVVGHFNSGVSIPTSQDYEEAGVLQISPASTNPTFTERKMWNTFR
TCGRDDQQGAVAGAYIVKNFKGKKVAIVHDKTPYGKGLADETQKTINKGGVKEVLYEGINPGEKDYSALVSKLKANGVDL
LYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRNNPAAKDIVAAFKAKGVDPEGYV
LYSYAAVQVMKQAAEAAKSLDPKKVAAEIHSGKTFNTVLGPLSFDKKGDITKLDYVVYVWKDGAYTQL
>Mature_388_residues
MRRGVSLGGGPVFRSRTKGRDLMKKLLMASLALGASLLLAAEAQAQVKMGVGGPMTGANAAFGAQLKTGAEQAVADINAA
GGILGQKIQLSVGDDGGKPEQGKSAANKFISDGVKFVVGHFNSGVSIPTSQDYEEAGVLQISPASTNPTFTERKMWNTFR
TCGRDDQQGAVAGAYIVKNFKGKKVAIVHDKTPYGKGLADETQKTINKGGVKEVLYEGINPGEKDYSALVSKLKANGVDL
LYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRNNPAAKDIVAAFKAKGVDPEGYV
LYSYAAVQVMKQAAEAAKSLDPKKVAAEIHSGKTFNTVLGPLSFDKKGDITKLDYVVYVWKDGAYTQL

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=358, Percent_Identity=43.0167597765363, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=40, Blast_Score=261, Evalue=5e-71,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41050; Mature: 41050

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGVSLGGGPVFRSRTKGRDLMKKLLMASLALGASLLLAAEAQAQVKMGVGGPMTGANA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCC
AFGAQLKTGAEQAVADINAAGGILGQKIQLSVGDDGGKPEQGKSAANKFISDGVKFVVGH
CCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHEEEEE
FNSGVSIPTSQDYEEAGVLQISPASTNPTFTERKMWNTFRTCGRDDQQGAVAGAYIVKNF
CCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCC
KGKKVAIVHDKTPYGKGLADETQKTINKGGVKEVLYEGINPGEKDYSALVSKLKANGVDL
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
LYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRNNP
EEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCC
AAKDIVAAFKAKGVDPEGYVLYSYAAVQVMKQAAEAAKSLDPKKVAAEIHSGKTFNTVLG
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHC
PLSFDKKGDITKLDYVVYVWKDGAYTQL
CCCCCCCCCCEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MRRGVSLGGGPVFRSRTKGRDLMKKLLMASLALGASLLLAAEAQAQVKMGVGGPMTGANA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCC
AFGAQLKTGAEQAVADINAAGGILGQKIQLSVGDDGGKPEQGKSAANKFISDGVKFVVGH
CCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHEEEEE
FNSGVSIPTSQDYEEAGVLQISPASTNPTFTERKMWNTFRTCGRDDQQGAVAGAYIVKNF
CCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCC
KGKKVAIVHDKTPYGKGLADETQKTINKGGVKEVLYEGINPGEKDYSALVSKLKANGVDL
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCEEE
LYYGGLHPEAGLLVRQMRDQGMKTVLFSGDGITDKEYWTIAGPGAEGTLMTFGPDPRNNP
EEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCC
AAKDIVAAFKAKGVDPEGYVLYSYAAVQVMKQAAEAAKSLDPKKVAAEIHSGKTFNTVLG
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHC
PLSFDKKGDITKLDYVVYVWKDGAYTQL
CCCCCCCCCCEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA