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The map label for this gene is ilvB [H]

Identifier: 154246734

GI number: 154246734

Start: 3112519

End: 3114237

Strand: Reverse

Name: ilvB [H]

Synonym: Xaut_2795

Alternate gene names: 154246734

Gene position: 3114237-3112519 (Counterclockwise)

Preceding gene: 154246735

Following gene: 154246732

Centisome position: 58.66

GC content: 71.38

Gene sequence:

>1719_bases
ATGCAGCATTCCGCGAACATCCGCTCCGGCGGCGAGATCCTGGTGGACGCGCTGAAGATCCATGGCGTCGACACGGTCTA
TTGCCTGCCCGGCGAGAGCTTCCTGCCCGCCATCGACGCCCTCGCGGGGGCCACCGGCCGCATCCGGACCATCGTGACCC
GCCACGAGGCCGCGGCGGCCAACATGGCGGAGGCCTACGGCAAGCTCACCGGACGGCCCGGCATCTGCTTCGTCACGCGC
GGGCCGGGCGCAAGCCATGCCGCCATCGGCATCCACACCGCCGCGCAGGATTCCACCCCGCTCATCCTCTTCATCGGGCA
GGTGGACACCGCCATCCGCGGCCGCGAGGCGTGGCAGGAGGTGGATTATCCCGCCATGTTCGGCGGCATGGCCAAGTGGG
TGGTGGAGGTGGACCGGCCGGAGCGCCTGCCCGAGCTGGTCTCCCGTGCCTTCCATGTGGCGGTTTCCGGCCGCCCCGGC
CCGGTGGTGGTGGCCCTGCCCGAGGACGTGCTGGGCGCCTCGGCGCAGGTGGCGGACGCGCAGGCGTACAAGCGCATCGC
CGCCCATCCCGGTGCGGACGACCTTAACCGCCTCACCGCCATGCTGGAGGCCAGCGAACGGCCCCTCGTCATCGTCGGCG
GCGGCGGATGGAGCGCCAGGGCCTGCCGCGACTTCCAGCATTTCGTGGAGGACTTCGACCTGCCGGTGGCGGCGGCCTTC
CGCCGCCAGGACATCCTCGACAACCACCACCCCAATTATGTGGGCCATGCGGGGCTCGGGCCGAACCCCAAGCTGGTGGC
CCGCATCCGCGCTGCCGACCTGATCGTCGCTATCGGCCCCCGCCTCGGCGAGACCACCACCGCCGGCTACACCCTGTTCG
AGCTGCCGCGCCCGGCGCAGCGCTTCGTCCATGTCCATGCCGACCCGCTGGAACTGGGCCGGGTCTATCAGGCGGACCTT
CCCATCAACGCCGGCATGCCCGAGTTCGCCGCCGCTGCCGCCGCGCTGAAGCCCAGGGGCGCCGCCGGCTGGCACGCGCC
GTGGAGCGCCGCCGTGAAGGCCGCCCGCGCCGACTATCTCGCCGACCAGACCCCCGGCGCCATGCCGGGCCGGCTCGATC
TCGGCGCGGTGATGGTGCACCTGCGCGAGAGCCTGCCCAGGGATGCCATCCTCACCAACGGCGCCGGCAACTACGCCATC
TGGGTGCACAGGTTCCACCGCTACGGCGGCTTCCGCACCCAGCTTGCTCCCACCTCCGGCGCCATGGGCTACGGCCTGCC
GGCCGCCATCGCCGCCAAGCTGCACCAGCCGGCACGGGACGTGATCTGCTTTGCCGGCGACGGCTGCTTCCAGATGAGCG
GGCAGGAACTGGCCACCGGCTGCCAGTACGGCGCCGGCGTGGTGGTGCTGCTGGTGAACAACGGCATCTATGGCTCCATC
CGCATGCACCAGGAGAGGGAATATCCCGGCCGGGTGTTCGCCACCACGCTGGAGAACCCCGACTTTCCTGCCCTGGCGCG
GGCCTATGGCGCCTTTGGCGAGCGGGTGGAGGAGACGGCGGCCTTCGCCGAGGCATTTAATCGCGCCCGCGCCTTCGCGG
CGGACCGGAACAAGCCGGCGTTGCTGGAGCTGGTCATCGATCCCGAAGCCATCACGCCGTCCGCCACCCTCACCGGCCTG
CGCGAGGCCGCGCTGAAGGCCGGCCAGGGCGCGACTTGA

Upstream 100 bases:

>100_bases
GCATGGTGCCGGAGACCAAGGGCAATTTCCGCTAAAGTTGAAGCCTGCGCCGGTGTCCCTCATCCGGAGCAGGCCCTCCC
TTCCCTGCACGGGCCAGACA

Downstream 100 bases:

>100_bases
AGCCGGCCAACGGCGGGGAGGACCGGGCACGAACCCGTCACCGCCATGCGGACGCACTCCGTCATCCAAGGCGCCTGGCA
GGGCGCCGCCTCGGGCGAGA

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MQHSANIRSGGEILVDALKIHGVDTVYCLPGESFLPAIDALAGATGRIRTIVTRHEAAAANMAEAYGKLTGRPGICFVTR
GPGASHAAIGIHTAAQDSTPLILFIGQVDTAIRGREAWQEVDYPAMFGGMAKWVVEVDRPERLPELVSRAFHVAVSGRPG
PVVVALPEDVLGASAQVADAQAYKRIAAHPGADDLNRLTAMLEASERPLVIVGGGGWSARACRDFQHFVEDFDLPVAAAF
RRQDILDNHHPNYVGHAGLGPNPKLVARIRAADLIVAIGPRLGETTTAGYTLFELPRPAQRFVHVHADPLELGRVYQADL
PINAGMPEFAAAAAALKPRGAAGWHAPWSAAVKAARADYLADQTPGAMPGRLDLGAVMVHLRESLPRDAILTNGAGNYAI
WVHRFHRYGGFRTQLAPTSGAMGYGLPAAIAAKLHQPARDVICFAGDGCFQMSGQELATGCQYGAGVVVLLVNNGIYGSI
RMHQEREYPGRVFATTLENPDFPALARAYGAFGERVEETAAFAEAFNRARAFAADRNKPALLELVIDPEAITPSATLTGL
REAALKAGQGAT

Sequences:

>Translated_572_residues
MQHSANIRSGGEILVDALKIHGVDTVYCLPGESFLPAIDALAGATGRIRTIVTRHEAAAANMAEAYGKLTGRPGICFVTR
GPGASHAAIGIHTAAQDSTPLILFIGQVDTAIRGREAWQEVDYPAMFGGMAKWVVEVDRPERLPELVSRAFHVAVSGRPG
PVVVALPEDVLGASAQVADAQAYKRIAAHPGADDLNRLTAMLEASERPLVIVGGGGWSARACRDFQHFVEDFDLPVAAAF
RRQDILDNHHPNYVGHAGLGPNPKLVARIRAADLIVAIGPRLGETTTAGYTLFELPRPAQRFVHVHADPLELGRVYQADL
PINAGMPEFAAAAAALKPRGAAGWHAPWSAAVKAARADYLADQTPGAMPGRLDLGAVMVHLRESLPRDAILTNGAGNYAI
WVHRFHRYGGFRTQLAPTSGAMGYGLPAAIAAKLHQPARDVICFAGDGCFQMSGQELATGCQYGAGVVVLLVNNGIYGSI
RMHQEREYPGRVFATTLENPDFPALARAYGAFGERVEETAAFAEAFNRARAFAADRNKPALLELVIDPEAITPSATLTGL
REAALKAGQGAT
>Mature_572_residues
MQHSANIRSGGEILVDALKIHGVDTVYCLPGESFLPAIDALAGATGRIRTIVTRHEAAAANMAEAYGKLTGRPGICFVTR
GPGASHAAIGIHTAAQDSTPLILFIGQVDTAIRGREAWQEVDYPAMFGGMAKWVVEVDRPERLPELVSRAFHVAVSGRPG
PVVVALPEDVLGASAQVADAQAYKRIAAHPGADDLNRLTAMLEASERPLVIVGGGGWSARACRDFQHFVEDFDLPVAAAF
RRQDILDNHHPNYVGHAGLGPNPKLVARIRAADLIVAIGPRLGETTTAGYTLFELPRPAQRFVHVHADPLELGRVYQADL
PINAGMPEFAAAAAALKPRGAAGWHAPWSAAVKAARADYLADQTPGAMPGRLDLGAVMVHLRESLPRDAILTNGAGNYAI
WVHRFHRYGGFRTQLAPTSGAMGYGLPAAIAAKLHQPARDVICFAGDGCFQMSGQELATGCQYGAGVVVLLVNNGIYGSI
RMHQEREYPGRVFATTLENPDFPALARAYGAFGERVEETAAFAEAFNRARAFAADRNKPALLELVIDPEAITPSATLTGL
REAALKAGQGAT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=26.8292682926829, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI21361361, Length=558, Percent_Identity=25.4480286738351, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=30.2325581395349, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=30.7553956834532, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=27.6173285198556, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1788716, Length=584, Percent_Identity=26.027397260274, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=25.2204585537919, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=26.63139329806, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=26.63139329806, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17542570, Length=593, Percent_Identity=24.283305227656, Blast_Score=120, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=27.5741710296684, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=23.1182795698925, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922626, Length=585, Percent_Identity=27.8632478632479, Blast_Score=168, Evalue=8e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60771; Mature: 60771

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHSANIRSGGEILVDALKIHGVDTVYCLPGESFLPAIDALAGATGRIRTIVTRHEAAAA
CCCCCCCCCCCHHEEEHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHH
NMAEAYGKLTGRPGICFVTRGPGASHAAIGIHTAAQDSTPLILFIGQVDTAIRGREAWQE
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHH
VDYPAMFGGMAKWVVEVDRPERLPELVSRAFHVAVSGRPGPVVVALPEDVLGASAQVADA
CCCHHHHHHHEEEEEECCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCCHHHHH
QAYKRIAAHPGADDLNRLTAMLEASERPLVIVGGGGWSARACRDFQHFVEDFDLPVAAAF
HHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RRQDILDNHHPNYVGHAGLGPNPKLVARIRAADLIVAIGPRLGETTTAGYTLFELPRPAQ
HHHHHHCCCCCCCEECCCCCCCHHHEEEEEHHEEEEEECCCCCCCCCCCEEEEECCCCHH
RFVHVHADPLELGRVYQADLPINAGMPEFAAAAAALKPRGAAGWHAPWSAAVKAARADYL
HEEEECCCHHHHCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ADQTPGAMPGRLDLGAVMVHLRESLPRDAILTNGAGNYAIWVHRFHRYGGFRTQLAPTSG
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEHHHHCCCEEEEECCCCC
AMGYGLPAAIAAKLHQPARDVICFAGDGCFQMSGQELATGCQYGAGVVVLLVNNGIYGSI
CCCCCCHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHCCCCCCCEEEEEECCCEEEEE
RMHQEREYPGRVFATTLENPDFPALARAYGAFGERVEETAAFAEAFNRARAFAADRNKPA
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLELVIDPEAITPSATLTGLREAALKAGQGAT
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQHSANIRSGGEILVDALKIHGVDTVYCLPGESFLPAIDALAGATGRIRTIVTRHEAAAA
CCCCCCCCCCCHHEEEHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHH
NMAEAYGKLTGRPGICFVTRGPGASHAAIGIHTAAQDSTPLILFIGQVDTAIRGREAWQE
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHH
VDYPAMFGGMAKWVVEVDRPERLPELVSRAFHVAVSGRPGPVVVALPEDVLGASAQVADA
CCCHHHHHHHEEEEEECCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCCHHHHH
QAYKRIAAHPGADDLNRLTAMLEASERPLVIVGGGGWSARACRDFQHFVEDFDLPVAAAF
HHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RRQDILDNHHPNYVGHAGLGPNPKLVARIRAADLIVAIGPRLGETTTAGYTLFELPRPAQ
HHHHHHCCCCCCCEECCCCCCCHHHEEEEEHHEEEEEECCCCCCCCCCCEEEEECCCCHH
RFVHVHADPLELGRVYQADLPINAGMPEFAAAAAALKPRGAAGWHAPWSAAVKAARADYL
HEEEECCCHHHHCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ADQTPGAMPGRLDLGAVMVHLRESLPRDAILTNGAGNYAIWVHRFHRYGGFRTQLAPTSG
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEHHHHCCCEEEEECCCCC
AMGYGLPAAIAAKLHQPARDVICFAGDGCFQMSGQELATGCQYGAGVVVLLVNNGIYGSI
CCCCCCHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHCCCCCCCEEEEEECCCEEEEE
RMHQEREYPGRVFATTLENPDFPALARAYGAFGERVEETAAFAEAFNRARAFAADRNKPA
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLELVIDPEAITPSATLTGLREAALKAGQGAT
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]