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The map label for this gene is nrtB [H]

Identifier: 154246715

GI number: 154246715

Start: 3073196

End: 3074254

Strand: Reverse

Name: nrtB [H]

Synonym: Xaut_2776

Alternate gene names: 154246715

Gene position: 3074254-3073196 (Counterclockwise)

Preceding gene: 154246724

Following gene: 154246714

Centisome position: 57.91

GC content: 66.86

Gene sequence:

>1059_bases
ATGACTGACCAGCTCGTTGAGGCCGCCAGCAGCCATGCCGGGTCCGTGGCCCCAAATAGATCCAGAAACCCAGCCAAATC
GAGAACCGCACGTAAATCCAGAAACGCCGCCCGCCCCAAGGTCTGGGCCACCGGCCTCGTCGCCGGTGCAGCGTGGCTCG
CGGCCGCCGCCTATGCCGATTTCGTGCCCGATGCGGACGACTTCGCCTACACGCAGTATTTCGCGGTGGGCCTTGCCGCC
ATCGGTGCCGCGCTCATTGTCCTCAGCCTGTGCGGCCCGGTCACCGGCCTGCCGCTCAAGGGCCTGCGCTATTGGGGACC
CTGGCTGGTGGTGATCCCGATCGGCCTGTTTGCCTGGGAGGCGGTGACCGCCAAGCTCGAACTGTTGCCCCGCCCGTTCT
TCGTGCCGCCCCAGTCCCTGATCGAGGTCTATCTGGATGACTGGCCGAAGCTCGGCCTCAGCACGGTGGCTTCGCTGAAA
CTGCTGGCCTGGGGCTACGGCATCGGCGCTGCGGTCGGCGTCACCCTCGGGGTCGCCATCGGCTGGTGGCGCACCGCCGG
CTACTGGGCGCATCCGGTGCTGCGCTTCATCGGCCCGTTGCCCGCCACCGCGTGGCTGCCGGTGGCGTTCTTCCTGTTCC
CCTCCAGCTGGAGCGCCAGCGTGTTCCTGCTGGCGCTGGCGACCGGCTTTCCCGTCACCATCCTCACCTGGTCGGGCATC
GCCAGCGTCAACAAGGATTATTATGACATCGCCCGCACGCTGGGCGCCAGCCAGCGCTTCCTGGTGCTCAAGGTGGCCAT
TCCCGCCGCCTTGCCCCACGTGTTCGTGGGCCTGTTCATGGGCCTCGGCACCTCCTTCGCGGTGCTGGTGGTGGCGGAGA
TGCTGGGCGTGAAGGCCGGGCTCGGCTGGTACCTGCAATGGGCGCAGGGCTGGGCTGCCTATGCCAACATGTATGCCGCC
CTCATCGTCATGGCGCTCATGTGCTCGGGCCTCGTCACCCTGCTGTTCAAGATCCGCGATCATCTTCTCTCCTGGCAGAA
GGAGCTGGTGCGGTGGTGA

Upstream 100 bases:

>100_bases
GCCGAGGAACTGAGAGTGGTCCAGGTTTTCACTCCGACAACCAACGTCCGTCGCTTTGCGGAAGACGTCGATGCGGACGT
CCTGAGCTGAGGAGAATGAG

Downstream 100 bases:

>100_bases
TCGAAGCGCTCTCCCCCACCCGCGCCCCCGCCAGCAACTGCGGCCCCGGCATCCGCCTCGATGTGGAGAATGTGAGCCAC
GCCTTCAGCCTGGAGGGCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTDQLVEAASSHAGSVAPNRSRNPAKSRTARKSRNAARPKVWATGLVAGAAWLAAAAYADFVPDADDFAYTQYFAVGLAA
IGAALIVLSLCGPVTGLPLKGLRYWGPWLVVIPIGLFAWEAVTAKLELLPRPFFVPPQSLIEVYLDDWPKLGLSTVASLK
LLAWGYGIGAAVGVTLGVAIGWWRTAGYWAHPVLRFIGPLPATAWLPVAFFLFPSSWSASVFLLALATGFPVTILTWSGI
ASVNKDYYDIARTLGASQRFLVLKVAIPAALPHVFVGLFMGLGTSFAVLVVAEMLGVKAGLGWYLQWAQGWAAYANMYAA
LIVMALMCSGLVTLLFKIRDHLLSWQKELVRW

Sequences:

>Translated_352_residues
MTDQLVEAASSHAGSVAPNRSRNPAKSRTARKSRNAARPKVWATGLVAGAAWLAAAAYADFVPDADDFAYTQYFAVGLAA
IGAALIVLSLCGPVTGLPLKGLRYWGPWLVVIPIGLFAWEAVTAKLELLPRPFFVPPQSLIEVYLDDWPKLGLSTVASLK
LLAWGYGIGAAVGVTLGVAIGWWRTAGYWAHPVLRFIGPLPATAWLPVAFFLFPSSWSASVFLLALATGFPVTILTWSGI
ASVNKDYYDIARTLGASQRFLVLKVAIPAALPHVFVGLFMGLGTSFAVLVVAEMLGVKAGLGWYLQWAQGWAAYANMYAA
LIVMALMCSGLVTLLFKIRDHLLSWQKELVRW
>Mature_351_residues
TDQLVEAASSHAGSVAPNRSRNPAKSRTARKSRNAARPKVWATGLVAGAAWLAAAAYADFVPDADDFAYTQYFAVGLAAI
GAALIVLSLCGPVTGLPLKGLRYWGPWLVVIPIGLFAWEAVTAKLELLPRPFFVPPQSLIEVYLDDWPKLGLSTVASLKL
LAWGYGIGAAVGVTLGVAIGWWRTAGYWAHPVLRFIGPLPATAWLPVAFFLFPSSWSASVFLLALATGFPVTILTWSGIA
SVNKDYYDIARTLGASQRFLVLKVAIPAALPHVFVGLFMGLGTSFAVLVVAEMLGVKAGLGWYLQWAQGWAAYANMYAAL
IVMALMCSGLVTLLFKIRDHLLSWQKELVRW

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=192, Percent_Identity=29.6875, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081802, Length=134, Percent_Identity=32.089552238806, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37981; Mature: 37850

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQLVEAASSHAGSVAPNRSRNPAKSRTARKSRNAARPKVWATGLVAGAAWLAAAAYAD
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
FVPDADDFAYTQYFAVGLAAIGAALIVLSLCGPVTGLPLKGLRYWGPWLVVIPIGLFAWE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
AVTAKLELLPRPFFVPPQSLIEVYLDDWPKLGLSTVASLKLLAWGYGIGAAVGVTLGVAI
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GWWRTAGYWAHPVLRFIGPLPATAWLPVAFFLFPSSWSASVFLLALATGFPVTILTWSGI
HHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCH
ASVNKDYYDIARTLGASQRFLVLKVAIPAALPHVFVGLFMGLGTSFAVLVVAEMLGVKAG
HHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
LGWYLQWAQGWAAYANMYAALIVMALMCSGLVTLLFKIRDHLLSWQKELVRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDQLVEAASSHAGSVAPNRSRNPAKSRTARKSRNAARPKVWATGLVAGAAWLAAAAYAD
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
FVPDADDFAYTQYFAVGLAAIGAALIVLSLCGPVTGLPLKGLRYWGPWLVVIPIGLFAWE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
AVTAKLELLPRPFFVPPQSLIEVYLDDWPKLGLSTVASLKLLAWGYGIGAAVGVTLGVAI
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GWWRTAGYWAHPVLRFIGPLPATAWLPVAFFLFPSSWSASVFLLALATGFPVTILTWSGI
HHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCH
ASVNKDYYDIARTLGASQRFLVLKVAIPAALPHVFVGLFMGLGTSFAVLVVAEMLGVKAG
HHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
LGWYLQWAQGWAAYANMYAALIVMALMCSGLVTLLFKIRDHLLSWQKELVRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8437564 [H]