Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 154246637

GI number: 154246637

Start: 2986247

End: 2987155

Strand: Reverse

Name: galE [C]

Synonym: Xaut_2697

Alternate gene names: 154246637

Gene position: 2987155-2986247 (Counterclockwise)

Preceding gene: 154246638

Following gene: 154246635

Centisome position: 56.27

GC content: 71.62

Gene sequence:

>909_bases
ATGAAGGTCCTCGTCACCGGCGGCACCGGATTCATCGCCGCCTGGATCATCCGCCGCCTGCTCCGGCGCAGCGTCGCCGT
GCGCGTCCTCGACATCGCCAGCGACAGCGCCGCCGTGCGCGACATCCTGGGCGACCTCGCACGCGAGGTGGATTGGCGGT
TGGCCGATATCCGCGACACAGACGCCGTGCATGCGGCCGCGCGGGGCTGCGGCGGGATCGTGCACATGGCCGGCATCCTC
ACGCCCGCCTGCCGGTCGGATCCGGTGAAGGGCGCCCAGATCAACCTCATCGGCACGCTCAACGCCTTCGAGGCCGCCCG
GCGCGAGGGCATGCCGCGCGTCGTCTATGCGAGTTCGGCGGGCGTGTTCGGGCCGCGGGACGGACACATCCCCTGCCCCA
CCACCCACTACGGCGCCTTCAAGCTCGCCTGCGAAGGCAGCGCCCGGGCGTATTGGGAAGACCATCGCATCGCCAGCATC
GGCTTCCGGCCCTTCATCGTCTACGGGCCGGGCCGCGGCGAGGAAGGCGCCAGCGCCGGGCCGACGCTTGCCTGCCGCGC
CGCCGCGCAGGGCGCGCCCTATGTGATCCCCTATGTGGGCTCAACCGGCATGATCTATGTGGACGACGTGGCGGTGGCCT
TCGAGCAGGCTCTGTTTGCCCCGCTGCCAGGCGCGCACGCCTTCAATCTGGCCGGACGCCCCGCGACCGTCGATGCGGTG
ATCGCCGCCATCCGGCGTGAGGTGCCCGATGCGCGCATCACCGCCACCGGCGCTCCCATGCCCATCGCCGGGGAGCTGGC
GCCGGATCACCTTTCCTCCGTGTTGCCGGACTTGCCGGAAACGTCCCTCGACACCGGCATCGCCGCCACCATCGCCTATT
ACCGGGGGCGGGCCGCCGCGCGGGCGTGA

Upstream 100 bases:

>100_bases
CGTCGTGGATGGCGGCCTCAGGCGCTCCCTCATGGGCCACATCCCGAGACCGGGATACTAAGGCGCCCCCTCCAACCACG
ATTGGACCAGAGGTTCAACC

Downstream 100 bases:

>100_bases
GCGCCTGCGTCCTATCGCGGTGCGGCGCGGAACGCTGCCGGTGAGCGCCCGGCATGGCGGGTGAAGAAGCGGGAGAAATA
AGCCGGATCCTGAAAGCCAA

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: UDP-Glucose 4-Epimerase; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase Family Protein; DTDP-Glucose 4 6-Dehydratase; GDP-D-Mannose Dehydratase; Epimerase; Nucleoside-Diphosphate-Sugar Epimerases; Nucleotide Sugar Epimerase/Dehydratase; NAD_dependent Epimerase/Dehydratase; NAD Dependent Epimerase; UDP-Glucose 4-Epimerase Related Protein; NAD Dependent Epimerase/Dehydratase Family; NDP-Sugar Dehydratase Or Epimerase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKVLVTGGTGFIAAWIIRRLLRRSVAVRVLDIASDSAAVRDILGDLAREVDWRLADIRDTDAVHAAARGCGGIVHMAGIL
TPACRSDPVKGAQINLIGTLNAFEAARREGMPRVVYASSAGVFGPRDGHIPCPTTHYGAFKLACEGSARAYWEDHRIASI
GFRPFIVYGPGRGEEGASAGPTLACRAAAQGAPYVIPYVGSTGMIYVDDVAVAFEQALFAPLPGAHAFNLAGRPATVDAV
IAAIRREVPDARITATGAPMPIAGELAPDHLSSVLPDLPETSLDTGIAATIAYYRGRAAARA

Sequences:

>Translated_302_residues
MKVLVTGGTGFIAAWIIRRLLRRSVAVRVLDIASDSAAVRDILGDLAREVDWRLADIRDTDAVHAAARGCGGIVHMAGIL
TPACRSDPVKGAQINLIGTLNAFEAARREGMPRVVYASSAGVFGPRDGHIPCPTTHYGAFKLACEGSARAYWEDHRIASI
GFRPFIVYGPGRGEEGASAGPTLACRAAAQGAPYVIPYVGSTGMIYVDDVAVAFEQALFAPLPGAHAFNLAGRPATVDAV
IAAIRREVPDARITATGAPMPIAGELAPDHLSSVLPDLPETSLDTGIAATIAYYRGRAAARA
>Mature_302_residues
MKVLVTGGTGFIAAWIIRRLLRRSVAVRVLDIASDSAAVRDILGDLAREVDWRLADIRDTDAVHAAARGCGGIVHMAGIL
TPACRSDPVKGAQINLIGTLNAFEAARREGMPRVVYASSAGVFGPRDGHIPCPTTHYGAFKLACEGSARAYWEDHRIASI
GFRPFIVYGPGRGEEGASAGPTLACRAAAQGAPYVIPYVGSTGMIYVDDVAVAFEQALFAPLPGAHAFNLAGRPATVDAV
IAAIRREVPDARITATGAPMPIAGELAPDHLSSVLPDLPETSLDTGIAATIAYYRGRAAARA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 31624; Mature: 31624

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGGTGFIAAWIIRRLLRRSVAVRVLDIASDSAAVRDILGDLAREVDWRLADIRDT
CEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCC
DAVHAAARGCGGIVHMAGILTPACRSDPVKGAQINLIGTLNAFEAARREGMPRVVYASSA
HHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCC
GVFGPRDGHIPCPTTHYGAFKLACEGSARAYWEDHRIASIGFRPFIVYGPGRGEEGASAG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCEEEEECCCEEEEECCCCCCCCCCCC
PTLACRAAAQGAPYVIPYVGSTGMIYVDDVAVAFEQALFAPLPGAHAFNLAGRPATVDAV
CCEEEHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
IAAIRREVPDARITATGAPMPIAGELAPDHLSSVLPDLPETSLDTGIAATIAYYRGRAAA
HHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHCCHHC
RA
CC
>Mature Secondary Structure
MKVLVTGGTGFIAAWIIRRLLRRSVAVRVLDIASDSAAVRDILGDLAREVDWRLADIRDT
CEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCC
DAVHAAARGCGGIVHMAGILTPACRSDPVKGAQINLIGTLNAFEAARREGMPRVVYASSA
HHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCC
GVFGPRDGHIPCPTTHYGAFKLACEGSARAYWEDHRIASIGFRPFIVYGPGRGEEGASAG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCEEEEECCCEEEEECCCCCCCCCCCC
PTLACRAAAQGAPYVIPYVGSTGMIYVDDVAVAFEQALFAPLPGAHAFNLAGRPATVDAV
CCEEEHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHH
IAAIRREVPDARITATGAPMPIAGELAPDHLSSVLPDLPETSLDTGIAATIAYYRGRAAA
HHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHCHHHHHHHHHHCCHHC
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA