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The map label for this gene is kstD [H]

Identifier: 154246635

GI number: 154246635

Start: 2983375

End: 2985087

Strand: Reverse

Name: kstD [H]

Synonym: Xaut_2695

Alternate gene names: 154246635

Gene position: 2985087-2983375 (Counterclockwise)

Preceding gene: 154246637

Following gene: 154246634

Centisome position: 56.23

GC content: 67.31

Gene sequence:

>1713_bases
ATGTCTTTGAGCGAACACGCCCCTCTCGTGTGCGACGTGCTGGTCATCGGGTCCGGAGCCGGCGGCCTGTCCACGGCGGT
CACCGCGAGGAAGCACGGCCTCGACGTCGTCGTGGTGGAGAAGGAGGAGCATTTCGGCGGCACCACCGCCTTTTCGGGCG
GCGTGCTGTGGATCCCTGGAAATCCCCATGCTGCGGCGCAGGGCATCACCGACACGCGAGACGCCGCCCGGCTCTACCTG
AAGACCGAGACCGGCAACTTCTACCGCGAGGACGCGATCGAGTACTTTCTCGACAACGGCCCGCGCATGCTCGACTTCTT
CGAGCGCGAGACCGAGGTGAAGTTCGTCGCCTCCGCCTATCCCGACTACCATCCGGATCTGCCGGGCGGCGTGGATATCG
GCCGCTCCGTCGTCGCCGCGCCCTATGATGCGCGCGGCCTTGGAGGCGAGATGAACCGGCTCAAGCCGCCGCTCGCGACC
ATCACCTTCATCGGCATGATGTTCAATTCATCCAACAATGAACTGAAGCATTTCTTCAACGCCACGCGCTCGCTGACCTC
CGCGCTCTATGTGGCGAAGCGCCTTGCCAATCACCTGAAGGACCTTCTTTTGTACCGGCGCGGGGTCCAGGTCACGAGCG
GCAACGCTCTGGCGGCCCGCCTCGCCAAATCGGCCTTCGACTGCGGCATTGCGATCCGCACCGGCACAGCCGCCGAAAAG
CTGATCGTCAAGGATGGCGCGGTGACCGGCGCCGTCGTGAAGGACGCCTCCGGCACCCAGCGCATCACCGCGCGACGGGG
GGTGGTGCTGGCCTGTGGCGGCTTCCCCCACGACGAGGCGCGCCTTGCGGGCATCTATCCCCACCGCCGGCGTGGCGGCG
CCCATGTCTCCCCCACCCCTGAGGGCAATACCGGCGATGGCATCCGCCTCGCGGAAACCGTGGGTGGGCAGTTCGAGGCG
CGCTTTCCCGATGCGGCGGCCTGGATGCCGGTGTCGAAAGTGCCCCTCGGCAACGGACGCTTCGGTGTATTCCCCCACCT
CGTGGACCGCTACAAGCCCGGCGTCATCGCGGTGAACCGGCACGGCCGCCGCTTCACCAACGAGGCCAATTCCTACCACG
ACGTGGGCGCCGCCATGATCGCCGACAGCGAGGGGGAGGGCGAGACCGTCGCCTGGCTGGTCTGCGACCATGCCACCCTC
CGCAAGTACGGCCTCGGCTTTGCCAAGCCCGCTCCGGTGCCGACCTCGCTCTATACCCGCTCCGGCTATCTGCTGAAGGG
TCGCACGCTGGCCGACCTCGCCCGCGCGGCCGGCATTGATGCCGCCGGCCTGGAAGAGACAGTGCGCACCTACAATGTGG
GCGCCGTAAAGGGCGAGGACCCGGCATTCCACCGCGGCTCCACCGCCTTCAACCGCTATCTCGGCGACCCCGACCACAAG
CCCAACCCCACGGTGGCGCCCATCGATCAAGGGCCGTTCTATGCGGTGAAGATCTTCATGGGTGATCTCGGTACCTTCGA
CGGGCTGTCCACCGACCCGCTCGGTCGGGTGCTGGACACGAACGCAGCCCCCATCGCCGGGCTCTACGCCGTGGGCAACG
ATGCGGCGAGCATCATGGGCGGCAACTATCCCGGCGCCGGCATCACGCTCGGGCCCATCATGACGTCGGGCTATGTCACC
GGGCGGCACCTGGCCGGCGTCTCCGCCCACTGA

Upstream 100 bases:

>100_bases
AGTGCGTCGGCACCGCGGTGCCATGCGATCACACAACGTCTTTTGCGCACAGGAAGCGCGGACTTCACACGCCCGGCGCG
TGTCGAGGAGGAGCCTTTTC

Downstream 100 bases:

>100_bases
CGAAAACAAAGATAAGACAATAAACCATAAGGGAAAAACGCCATGGCCATGGATCGCAGGAGCTTTCTCGGCACAAGCCT
TGCCGTTACCGCGTTCGGGC

Product: putative succinate dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSLSEHAPLVCDVLVIGSGAGGLSTAVTARKHGLDVVVVEKEEHFGGTTAFSGGVLWIPGNPHAAAQGITDTRDAARLYL
KTETGNFYREDAIEYFLDNGPRMLDFFERETEVKFVASAYPDYHPDLPGGVDIGRSVVAAPYDARGLGGEMNRLKPPLAT
ITFIGMMFNSSNNELKHFFNATRSLTSALYVAKRLANHLKDLLLYRRGVQVTSGNALAARLAKSAFDCGIAIRTGTAAEK
LIVKDGAVTGAVVKDASGTQRITARRGVVLACGGFPHDEARLAGIYPHRRRGGAHVSPTPEGNTGDGIRLAETVGGQFEA
RFPDAAAWMPVSKVPLGNGRFGVFPHLVDRYKPGVIAVNRHGRRFTNEANSYHDVGAAMIADSEGEGETVAWLVCDHATL
RKYGLGFAKPAPVPTSLYTRSGYLLKGRTLADLARAAGIDAAGLEETVRTYNVGAVKGEDPAFHRGSTAFNRYLGDPDHK
PNPTVAPIDQGPFYAVKIFMGDLGTFDGLSTDPLGRVLDTNAAPIAGLYAVGNDAASIMGGNYPGAGITLGPIMTSGYVT
GRHLAGVSAH

Sequences:

>Translated_570_residues
MSLSEHAPLVCDVLVIGSGAGGLSTAVTARKHGLDVVVVEKEEHFGGTTAFSGGVLWIPGNPHAAAQGITDTRDAARLYL
KTETGNFYREDAIEYFLDNGPRMLDFFERETEVKFVASAYPDYHPDLPGGVDIGRSVVAAPYDARGLGGEMNRLKPPLAT
ITFIGMMFNSSNNELKHFFNATRSLTSALYVAKRLANHLKDLLLYRRGVQVTSGNALAARLAKSAFDCGIAIRTGTAAEK
LIVKDGAVTGAVVKDASGTQRITARRGVVLACGGFPHDEARLAGIYPHRRRGGAHVSPTPEGNTGDGIRLAETVGGQFEA
RFPDAAAWMPVSKVPLGNGRFGVFPHLVDRYKPGVIAVNRHGRRFTNEANSYHDVGAAMIADSEGEGETVAWLVCDHATL
RKYGLGFAKPAPVPTSLYTRSGYLLKGRTLADLARAAGIDAAGLEETVRTYNVGAVKGEDPAFHRGSTAFNRYLGDPDHK
PNPTVAPIDQGPFYAVKIFMGDLGTFDGLSTDPLGRVLDTNAAPIAGLYAVGNDAASIMGGNYPGAGITLGPIMTSGYVT
GRHLAGVSAH
>Mature_569_residues
SLSEHAPLVCDVLVIGSGAGGLSTAVTARKHGLDVVVVEKEEHFGGTTAFSGGVLWIPGNPHAAAQGITDTRDAARLYLK
TETGNFYREDAIEYFLDNGPRMLDFFERETEVKFVASAYPDYHPDLPGGVDIGRSVVAAPYDARGLGGEMNRLKPPLATI
TFIGMMFNSSNNELKHFFNATRSLTSALYVAKRLANHLKDLLLYRRGVQVTSGNALAARLAKSAFDCGIAIRTGTAAEKL
IVKDGAVTGAVVKDASGTQRITARRGVVLACGGFPHDEARLAGIYPHRRRGGAHVSPTPEGNTGDGIRLAETVGGQFEAR
FPDAAAWMPVSKVPLGNGRFGVFPHLVDRYKPGVIAVNRHGRRFTNEANSYHDVGAAMIADSEGEGETVAWLVCDHATLR
KYGLGFAKPAPVPTSLYTRSGYLLKGRTLADLARAAGIDAAGLEETVRTYNVGAVKGEDPAFHRGSTAFNRYLGDPDHKP
NPTVAPIDQGPFYAVKIFMGDLGTFDGLSTDPLGRVLDTNAAPIAGLYAVGNDAASIMGGNYPGAGITLGPIMTSGYVTG
RHLAGVSAH

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 60392; Mature: 60261

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSEHAPLVCDVLVIGSGAGGLSTAVTARKHGLDVVVVEKEEHFGGTTAFSGGVLWIPG
CCCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCEEEECC
NPHAAAQGITDTRDAARLYLKTETGNFYREDAIEYFLDNGPRMLDFFERETEVKFVASAY
CCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECC
PDYHPDLPGGVDIGRSVVAAPYDARGLGGEMNRLKPPLATITFIGMMFNSSNNELKHFFN
CCCCCCCCCCHHHCCEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHEECCCCHHHHHHHH
ATRSLTSALYVAKRLANHLKDLLLYRRGVQVTSGNALAARLAKSAFDCGIAIRTGTAAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCEEEECCCCHHE
LIVKDGAVTGAVVKDASGTQRITARRGVVLACGGFPHDEARLAGIYPHRRRGGAHVSPTP
EEEECCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCEEECCCCHHCCCCCCCCCCC
EGNTGDGIRLAETVGGQFEARFPDAAAWMPVSKVPLGNGRFGVFPHLVDRYKPGVIAVNR
CCCCCCCEEEEHHCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HGRRFTNEANSYHDVGAAMIADSEGEGETVAWLVCDHATLRKYGLGFAKPAPVPTSLYTR
CCCCCCCCCCCHHHCCEEEEECCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCHHCCC
SGYLLKGRTLADLARAAGIDAAGLEETVRTYNVGAVKGEDPAFHRGSTAFNRYLGDPDHK
CCEEEECCHHHHHHHHCCCCHHHHHHHHHHEECCEECCCCCCCCCCCHHHHHHCCCCCCC
PNPTVAPIDQGPFYAVKIFMGDLGTFDGLSTDPLGRVLDTNAAPIAGLYAVGNDAASIMG
CCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHCC
GNYPGAGITLGPIMTSGYVTGRHLAGVSAH
CCCCCCCEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLSEHAPLVCDVLVIGSGAGGLSTAVTARKHGLDVVVVEKEEHFGGTTAFSGGVLWIPG
CCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCEEEECC
NPHAAAQGITDTRDAARLYLKTETGNFYREDAIEYFLDNGPRMLDFFERETEVKFVASAY
CCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECC
PDYHPDLPGGVDIGRSVVAAPYDARGLGGEMNRLKPPLATITFIGMMFNSSNNELKHFFN
CCCCCCCCCCHHHCCEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHEECCCCHHHHHHHH
ATRSLTSALYVAKRLANHLKDLLLYRRGVQVTSGNALAARLAKSAFDCGIAIRTGTAAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCEEEECCCCHHE
LIVKDGAVTGAVVKDASGTQRITARRGVVLACGGFPHDEARLAGIYPHRRRGGAHVSPTP
EEEECCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCEEECCCCHHCCCCCCCCCCC
EGNTGDGIRLAETVGGQFEARFPDAAAWMPVSKVPLGNGRFGVFPHLVDRYKPGVIAVNR
CCCCCCCEEEEHHCCCCEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HGRRFTNEANSYHDVGAAMIADSEGEGETVAWLVCDHATLRKYGLGFAKPAPVPTSLYTR
CCCCCCCCCCCHHHCCEEEEECCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCHHCCC
SGYLLKGRTLADLARAAGIDAAGLEETVRTYNVGAVKGEDPAFHRGSTAFNRYLGDPDHK
CCEEEECCHHHHHHHHCCCCHHHHHHHHHHEECCEECCCCCCCCCCCHHHHHHCCCCCCC
PNPTVAPIDQGPFYAVKIFMGDLGTFDGLSTDPLGRVLDTNAAPIAGLYAVGNDAASIMG
CCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHCC
GNYPGAGITLGPIMTSGYVTGRHLAGVSAH
CCCCCCCEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]