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The map label for this gene is hemK [H]

Identifier: 154246563

GI number: 154246563

Start: 2908112

End: 2908972

Strand: Reverse

Name: hemK [H]

Synonym: Xaut_2622

Alternate gene names: 154246563

Gene position: 2908972-2908112 (Counterclockwise)

Preceding gene: 154246564

Following gene: 154246562

Centisome position: 54.79

GC content: 74.33

Gene sequence:

>861_bases
GTGGCTGAAGCCCTGACCATCGGCACTCTGCGCCGGCAGATGGCGGCATCCCTCGCTGCGGCGGGCGTGGAGACGCCGGA
CCTCGACGCCCGCCTGCTGCTGGCGCAGGCGCTCGGCTGCGCGCCGGGCGATCTTCTCCTGCGCGCCGACGCGCCCGTCC
CGTCCGAGGCGGAGGTGACGGCGCGCGCCTTCACGAAACGACGGGCGGGCGGCGAGCCGGTGGCGCGCATTCGCGGCCGG
CGGGAGTTCTGGAGTCTCGACCTTCACCTCTCCCCGGACACCTTGGTGCCCCGCCCCGATACCGAGACCCTGGTGGAGGC
GGCCCTCGCCGCGCTGCCCGACCGAACCGCACCCCTCTCCATCCTCGATCTGGGCACGGGCACGGGCGCGATCCTCGCCG
CCTTGCTGGTGGAGTGTCCCAACGCCACCGGCATCGCCGTGGACCGCTCAGAGGGCGCGGCCTTGACCGCGCGCGCCAAT
CTCGCCCGCAACGGCCTCGCCGCGCGCGCCCATGTGATGGTCGGCGACTGGGGAACGGCGCTGGCCGGCGGCTTCGACCT
CGTGGTCTCCAACCCGCCCTACATCACCTCTTCGGCCATGCTGGACCTGCCCGCCGAAGTCCGCCTGCACGACCCTCATC
TCGCGTTGGAGGCCGGGGCGGACGGCCTCCTCGCCTATGAAGCCATCGCCGCCGACCTGCCGCGCCTCGTGCGCCGAGGC
GGCATCGCCGTGCTGGAGCTGGGCGAGGGACAGGAAGCGGCCGTGGCGGCGCTGGTCGCGGCGGCGGGACTTGAGGTGGC
AGGTCCGGCGCGGCGCGATCTTTCGGGCATCCCCAGGGCCCTCCTCGCCCGCCGCGGGTAG

Upstream 100 bases:

>100_bases
ACGGCGCTGGACGAGATCATCGATCCGCTGCTCACCGAACACCAGGCCGCTTTGCTCGCCGCGGCCGAGGACGCCTGAGC
CGTGGGCAAGGGCCCTGCTT

Downstream 100 bases:

>100_bases
GGCCAGGTAGCGGCAAATGGCGGCGCCCCAAGGCAAGCAGCGCGCAAGCTGAGAAAAAACCGCTTGGAACCTGGGAGGGA
AATGGCTAGTGTCTTTTTCA

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAEALTIGTLRRQMAASLAAAGVETPDLDARLLLAQALGCAPGDLLLRADAPVPSEAEVTARAFTKRRAGGEPVARIRGR
REFWSLDLHLSPDTLVPRPDTETLVEAALAALPDRTAPLSILDLGTGTGAILAALLVECPNATGIAVDRSEGAALTARAN
LARNGLAARAHVMVGDWGTALAGGFDLVVSNPPYITSSAMLDLPAEVRLHDPHLALEAGADGLLAYEAIAADLPRLVRRG
GIAVLELGEGQEAAVAALVAAAGLEVAGPARRDLSGIPRALLARRG

Sequences:

>Translated_286_residues
MAEALTIGTLRRQMAASLAAAGVETPDLDARLLLAQALGCAPGDLLLRADAPVPSEAEVTARAFTKRRAGGEPVARIRGR
REFWSLDLHLSPDTLVPRPDTETLVEAALAALPDRTAPLSILDLGTGTGAILAALLVECPNATGIAVDRSEGAALTARAN
LARNGLAARAHVMVGDWGTALAGGFDLVVSNPPYITSSAMLDLPAEVRLHDPHLALEAGADGLLAYEAIAADLPRLVRRG
GIAVLELGEGQEAAVAALVAAAGLEVAGPARRDLSGIPRALLARRG
>Mature_285_residues
AEALTIGTLRRQMAASLAAAGVETPDLDARLLLAQALGCAPGDLLLRADAPVPSEAEVTARAFTKRRAGGEPVARIRGRR
EFWSLDLHLSPDTLVPRPDTETLVEAALAALPDRTAPLSILDLGTGTGAILAALLVECPNATGIAVDRSEGAALTARANL
ARNGLAARAHVMVGDWGTALAGGFDLVVSNPPYITSSAMLDLPAEVRLHDPHLALEAGADGLLAYEAIAADLPRLVRRGG
IAVLELGEGQEAAVAALVAAAGLEVAGPARRDLSGIPRALLARRG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=33.0677290836653, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787463, Length=274, Percent_Identity=39.4160583941606, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=30.9644670050761, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=160, Percent_Identity=31.25, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582226, Length=267, Percent_Identity=26.9662921348315, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 29384; Mature: 29253

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEALTIGTLRRQMAASLAAAGVETPDLDARLLLAQALGCAPGDLLLRADAPVPSEAEVT
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
ARAFTKRRAGGEPVARIRGRREFWSLDLHLSPDTLVPRPDTETLVEAALAALPDRTAPLS
HHHHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
ILDLGTGTGAILAALLVECPNATGIAVDRSEGAALTARANLARNGLAARAHVMVGDWGTA
EEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEHHHHHCCCCEEEEEEECCCHHH
LAGGFDLVVSNPPYITSSAMLDLPAEVRLHDPHLALEAGADGLLAYEAIAADLPRLVRRG
HCCCEEEEECCCCCEECCCEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHC
GIAVLELGEGQEAAVAALVAAAGLEVAGPARRDLSGIPRALLARRG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AEALTIGTLRRQMAASLAAAGVETPDLDARLLLAQALGCAPGDLLLRADAPVPSEAEVT
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
ARAFTKRRAGGEPVARIRGRREFWSLDLHLSPDTLVPRPDTETLVEAALAALPDRTAPLS
HHHHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
ILDLGTGTGAILAALLVECPNATGIAVDRSEGAALTARANLARNGLAARAHVMVGDWGTA
EEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEHHHHHCCCCEEEEEEECCCHHH
LAGGFDLVVSNPPYITSSAMLDLPAEVRLHDPHLALEAGADGLLAYEAIAADLPRLVRRG
HCCCEEEEECCCCCEECCCEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHC
GIAVLELGEGQEAAVAALVAAAGLEVAGPARRDLSGIPRALLARRG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]