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The map label for this gene is trmD [H]

Identifier: 154246452

GI number: 154246452

Start: 2806083

End: 2806811

Strand: Reverse

Name: trmD [H]

Synonym: Xaut_2511

Alternate gene names: 154246452

Gene position: 2806811-2806083 (Counterclockwise)

Preceding gene: 154246453

Following gene: 154246451

Centisome position: 52.87

GC content: 71.47

Gene sequence:

>729_bases
ATGACCTCCCCGACCTTCCGCGCCAGCATCCTCACCCTTTACCCGGAGATGTTTCCCGGTCCTCTCGGCCATGCCCTGGC
CGGCCGGGCGCTGGAGAAGGGGCTGTGGGCGCTGGAGGCAGTGCAGATCCGCGACCATGCCCTCGACAAGCATCGCTCGG
TGGACGACACCCCCGCCGGCGGCGGCCCGGGCATGGTGATGCGCGCCGATGTGCTGGCGCGCGCCATCGATGCGGTCTCC
CCGCCCGATGATCCCCGCCCGCGCCTGCTCATGACCCCGCGCGGCGCCCCGCTCACCCAGGCGCGGGTGCGCGCCTTGTC
GGAAGGCCCCGGCGCGGTGATCCTGTGTGGGCGGTTCGAGGGGGTGGACGAGCGTATCGTCGGCGCCCGCAACCTTGAGG
AAATCTGCGTCGGCGACGTGGTGCTGTCCGGCGGCGAGGCGGCGGCGCTGCTGCTGCTCGATGCCTGCGTGCGGCTCATT
CCCGGCGTCATGGGCAAGGCGGAGAGCGGCACCGAGGAGAGCTTTTCCGCGGGCCTGCTGGAATATCCCCAATACACCCG
CCCGCAGCTGTTCGAGGGCGAGCCCATCCCCGACGTGCTGACCTGCGGCGATCACGCCCGCGTCGCCCGCTGGCGCCATG
CCCAATCCCTCGCCGTCACCCGCGCGCGGCGGCCGGATCTTCTCGCTCCCGTGGCCCCGCATGACGGGACCATGAAGGAA
AGGGAATGA

Upstream 100 bases:

>100_bases
GGGCGAGGACACTGGGGGCGAGTGAGCGCTGCGCATTCCCACTTGATGCGCAGAGGGGCACCCGTTTGACTCGGACGCCC
CCCTCCCCCATCACGCTGCC

Downstream 100 bases:

>100_bases
CACGGCCCCATTTCTCGGCTATGGAGCCGCGTCCCTTAACTTAAAACGACACAACGTGGCACCGGGGCGTTTTGCCGAAC
CGGTGGCTGCACAAAGGTTG

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTSPTFRASILTLYPEMFPGPLGHALAGRALEKGLWALEAVQIRDHALDKHRSVDDTPAGGGPGMVMRADVLARAIDAVS
PPDDPRPRLLMTPRGAPLTQARVRALSEGPGAVILCGRFEGVDERIVGARNLEEICVGDVVLSGGEAAALLLLDACVRLI
PGVMGKAESGTEESFSAGLLEYPQYTRPQLFEGEPIPDVLTCGDHARVARWRHAQSLAVTRARRPDLLAPVAPHDGTMKE
RE

Sequences:

>Translated_242_residues
MTSPTFRASILTLYPEMFPGPLGHALAGRALEKGLWALEAVQIRDHALDKHRSVDDTPAGGGPGMVMRADVLARAIDAVS
PPDDPRPRLLMTPRGAPLTQARVRALSEGPGAVILCGRFEGVDERIVGARNLEEICVGDVVLSGGEAAALLLLDACVRLI
PGVMGKAESGTEESFSAGLLEYPQYTRPQLFEGEPIPDVLTCGDHARVARWRHAQSLAVTRARRPDLLAPVAPHDGTMKE
RE
>Mature_241_residues
TSPTFRASILTLYPEMFPGPLGHALAGRALEKGLWALEAVQIRDHALDKHRSVDDTPAGGGPGMVMRADVLARAIDAVSP
PDDPRPRLLMTPRGAPLTQARVRALSEGPGAVILCGRFEGVDERIVGARNLEEICVGDVVLSGGEAAALLLLDACVRLIP
GVMGKAESGTEESFSAGLLEYPQYTRPQLFEGEPIPDVLTCGDHARVARWRHAQSLAVTRARRPDLLAPVAPHDGTMKER
E

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=44.1964285714286, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25933; Mature: 25802

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPTFRASILTLYPEMFPGPLGHALAGRALEKGLWALEAVQIRDHALDKHRSVDDTPAG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GGPGMVMRADVLARAIDAVSPPDDPRPRLLMTPRGAPLTQARVRALSEGPGAVILCGRFE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEECCC
GVDERIVGARNLEEICVGDVVLSGGEAAALLLLDACVRLIPGVMGKAESGTEESFSAGLL
CCHHHHHCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHH
EYPQYTRPQLFEGEPIPDVLTCGDHARVARWRHAQSLAVTRARRPDLLAPVAPHDGTMKE
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RE
CC
>Mature Secondary Structure 
TSPTFRASILTLYPEMFPGPLGHALAGRALEKGLWALEAVQIRDHALDKHRSVDDTPAG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GGPGMVMRADVLARAIDAVSPPDDPRPRLLMTPRGAPLTQARVRALSEGPGAVILCGRFE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEECCC
GVDERIVGARNLEEICVGDVVLSGGEAAALLLLDACVRLIPGVMGKAESGTEESFSAGLL
CCHHHHHCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHH
EYPQYTRPQLFEGEPIPDVLTCGDHARVARWRHAQSLAVTRARRPDLLAPVAPHDGTMKE
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]