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The map label for this gene is ybiS [H]

Identifier: 154246416

GI number: 154246416

Start: 2766759

End: 2767361

Strand: Reverse

Name: ybiS [H]

Synonym: Xaut_2475

Alternate gene names: 154246416

Gene position: 2767361-2766759 (Counterclockwise)

Preceding gene: 154246418

Following gene: 154246414

Centisome position: 52.13

GC content: 62.52

Gene sequence:

>603_bases
ATGATGTGGCGGGTGGTCGGAGCACTGCTGACAGGCTTGATGCTCAGCGGTTGCATGTCGGAAACCTATGCGCCGGCACC
TGAATCGGCGCAGACGGCGCGGGACAAGCAGCTTCTCGCGAACAAGCCGTATCCCAATGTGAAGCCGAGCGGAGATTACG
CACGGCATATCGTCGAGTATCCGACGAACAAGAAGCCGGGTACCATAGTCATCGATACGCCCAACAAGTTCCTTTACTAC
GTCGAAGGCAACGGCAAGGCGATCCGCTACGGTGTTACCGTGGGCGAGGAAAGCCTCGCGTTCCGCGGCCAGGCGAAGGT
GGGGCGCAAGGCGGAATGGCCGAGCTGGACCCCCACCCCGGAAATTCACCAGCGCATCGCCGGCCTGCCCTCCTATGTGG
CCCCCGGCCCGCACAACCCCATGGGTGCCCGCGCGCTGTACCTGTACCAGGGCAACCAGGACACGCTGTTCCGCATCCAC
GGCACCAACCAGCCTGAATATCTCGGGCAGGCCATTTCCTCGGGCTGCATCCGCATGCTGAACGAGGACGTGATCGACCT
CTACAGCCGCGTGCCGACCGGTACCGAAGTGGTGGTTCTCTGA

Upstream 100 bases:

>100_bases
GCATGCGCAGAACTCTTCAAGCATGGCGGACATTGGGTTAATTCAATCGGAGCGGGATCGATTCCTCCTTGCGCAGCAAG
GCGAGTAAAACGAGGCGAAC

Downstream 100 bases:

>100_bases
TCACGGCATGTGCGCCTGACCAGTAGAGAGTGATGTTTGACGAAGGGCGCGGTTGATCCGCGCCCTTTTTCTTTTGTGCC
TGTGATCCAGCAGCTTCAGC

Product: ErfK/YbiS/YcfS/YnhG family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MMWRVVGALLTGLMLSGCMSETYAPAPESAQTARDKQLLANKPYPNVKPSGDYARHIVEYPTNKKPGTIVIDTPNKFLYY
VEGNGKAIRYGVTVGEESLAFRGQAKVGRKAEWPSWTPTPEIHQRIAGLPSYVAPGPHNPMGARALYLYQGNQDTLFRIH
GTNQPEYLGQAISSGCIRMLNEDVIDLYSRVPTGTEVVVL

Sequences:

>Translated_200_residues
MMWRVVGALLTGLMLSGCMSETYAPAPESAQTARDKQLLANKPYPNVKPSGDYARHIVEYPTNKKPGTIVIDTPNKFLYY
VEGNGKAIRYGVTVGEESLAFRGQAKVGRKAEWPSWTPTPEIHQRIAGLPSYVAPGPHNPMGARALYLYQGNQDTLFRIH
GTNQPEYLGQAISSGCIRMLNEDVIDLYSRVPTGTEVVVL
>Mature_200_residues
MMWRVVGALLTGLMLSGCMSETYAPAPESAQTARDKQLLANKPYPNVKPSGDYARHIVEYPTNKKPGTIVIDTPNKFLYY
VEGNGKAIRYGVTVGEESLAFRGQAKVGRKAEWPSWTPTPEIHQRIAGLPSYVAPGPHNPMGARALYLYQGNQDTLFRIH
GTNQPEYLGQAISSGCIRMLNEDVIDLYSRVPTGTEVVVL

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=143, Percent_Identity=36.3636363636364, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787968, Length=145, Percent_Identity=39.3103448275862, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788299, Length=98, Percent_Identity=43.8775510204082, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787356, Length=149, Percent_Identity=31.5436241610738, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 22039; Mature: 22039

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMWRVVGALLTGLMLSGCMSETYAPAPESAQTARDKQLLANKPYPNVKPSGDYARHIVEY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
PTNKKPGTIVIDTPNKFLYYVEGNGKAIRYGVTVGEESLAFRGQAKVGRKAEWPSWTPTP
CCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCHHHEECCCCCCCCCCCCCCCCCCH
EIHQRIAGLPSYVAPGPHNPMGARALYLYQGNQDTLFRIHGTNQPEYLGQAISSGCIRML
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
NEDVIDLYSRVPTGTEVVVL
HHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MMWRVVGALLTGLMLSGCMSETYAPAPESAQTARDKQLLANKPYPNVKPSGDYARHIVEY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
PTNKKPGTIVIDTPNKFLYYVEGNGKAIRYGVTVGEESLAFRGQAKVGRKAEWPSWTPTP
CCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCHHHEECCCCCCCCCCCCCCCCCCH
EIHQRIAGLPSYVAPGPHNPMGARALYLYQGNQDTLFRIHGTNQPEYLGQAISSGCIRML
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
NEDVIDLYSRVPTGTEVVVL
HHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]