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The map label for this gene is rbsB [H]

Identifier: 154246354

GI number: 154246354

Start: 2694706

End: 2695749

Strand: Direct

Name: rbsB [H]

Synonym: Xaut_2413

Alternate gene names: 154246354

Gene position: 2694706-2695749 (Clockwise)

Preceding gene: 154246353

Following gene: 154246355

Centisome position: 50.76

GC content: 68.49

Gene sequence:

>1044_bases
ATGCACCTGTGGCCACGGCTGCTGATGGCAGCTTCTGTCGCCTGGGCCGGGCTGGCCGCGACATCGCCCGCCGGGGCTGC
GGAAAAGCCCGCAGACAAGCCCATCGTGGGCATCGTCATCAAAACCTCCACCAACCCCTTCTTCGTCAAGATGAAGGAGG
GCGCGGAAGCCGCCGCCGCGCGGCTGGGCCTGGAGCTGCGATCCTTCGCCGGCAAGATGGACGGCGACACCGATGCCCAG
GTCACCGCCGTCGAGAGCCTGATCGCCGCCGGCGCCAAGGGCATCTTGATCACCCCGTCGGATTCCCGCGCCATCGTGCC
GGTGCTGGAGCAGGCGCGGGCCAAGGGCATCCTGGTGATCGCCCTCGACACGCCCCTCGATCCCCCGGGCGCGGCGGACG
CCACCTTCGCCACCGACAATGTCCTCGCCGGCAAGCTGGTGGGCGAATGGGCGGCCAAGACTTTGGGCGCCAATGCCAAA
TCCGCCAAGGTCGCGCTGCTCGACCTGAACGTGAACCAGATTTCCGTGGACGTGGACCGCGACCAGGGCTTCCTCAAGGG
CTTCGGCGTGAACGTTGCCGACCCCGCCGTCATCGGCGACGAGAAGGACAGCCGCATCGTCGGCCACGATGTCACCCTCG
GCAATGAGGAGGGCGGGCGCCGGGCCATGGAGAACCTGCTCCAGCGCGATCCCTATGTGTCCGTGGTCTATGCCATCAAC
GAGCCCGCCGCCGCCGGCGCCTATGAGGCGCTGCGCTCCATCGGCCGCGACAAGGACGTGCTGGTGGTGGCCGTGGACGG
CGGCTGCCCTGGCGTGCGCAACGTGAAGGACGGCGTCATCGCCGCCACCGCCATGCAATTCCCCCTCGCCATGGCCCAGA
TGGGCGTCGAGGCGGTGCTGAAATACGCCACGGACGGCACCAAGCCGAAGCCCGCGGACGGGCAGGACTTCGTCAATACC
GGCGTGGAGCTGGTGACCGACCGGCCGGTGCCGGGACTGCCTTCCATCGACACCGACGCCGCCCTGAAGAAATGCTGGGG
CTGA

Upstream 100 bases:

>100_bases
TATCTATTGACTCGCCATGCGCCCTGCGCTCAGATGCCATGCGGATAACGCATCCGTCCGGCAAGCGGAAACCGCGCCGG
CGCATCTGGGAGGACGTTCG

Downstream 100 bases:

>100_bases
TGGGCCCCGTTAAGGCACGCGTGCGCCGGCCGAAACGGCCGGGGAGGGATCTGCAATGACAGACACGAGTGGGGAGAAAC
GCGGGATGCAGGCGTTCGAA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MHLWPRLLMAASVAWAGLAATSPAGAAEKPADKPIVGIVIKTSTNPFFVKMKEGAEAAAARLGLELRSFAGKMDGDTDAQ
VTAVESLIAAGAKGILITPSDSRAIVPVLEQARAKGILVIALDTPLDPPGAADATFATDNVLAGKLVGEWAAKTLGANAK
SAKVALLDLNVNQISVDVDRDQGFLKGFGVNVADPAVIGDEKDSRIVGHDVTLGNEEGGRRAMENLLQRDPYVSVVYAIN
EPAAAGAYEALRSIGRDKDVLVVAVDGGCPGVRNVKDGVIAATAMQFPLAMAQMGVEAVLKYATDGTKPKPADGQDFVNT
GVELVTDRPVPGLPSIDTDAALKKCWG

Sequences:

>Translated_347_residues
MHLWPRLLMAASVAWAGLAATSPAGAAEKPADKPIVGIVIKTSTNPFFVKMKEGAEAAAARLGLELRSFAGKMDGDTDAQ
VTAVESLIAAGAKGILITPSDSRAIVPVLEQARAKGILVIALDTPLDPPGAADATFATDNVLAGKLVGEWAAKTLGANAK
SAKVALLDLNVNQISVDVDRDQGFLKGFGVNVADPAVIGDEKDSRIVGHDVTLGNEEGGRRAMENLLQRDPYVSVVYAIN
EPAAAGAYEALRSIGRDKDVLVVAVDGGCPGVRNVKDGVIAATAMQFPLAMAQMGVEAVLKYATDGTKPKPADGQDFVNT
GVELVTDRPVPGLPSIDTDAALKKCWG
>Mature_347_residues
MHLWPRLLMAASVAWAGLAATSPAGAAEKPADKPIVGIVIKTSTNPFFVKMKEGAEAAAARLGLELRSFAGKMDGDTDAQ
VTAVESLIAAGAKGILITPSDSRAIVPVLEQARAKGILVIALDTPLDPPGAADATFATDNVLAGKLVGEWAAKTLGANAK
SAKVALLDLNVNQISVDVDRDQGFLKGFGVNVADPAVIGDEKDSRIVGHDVTLGNEEGGRRAMENLLQRDPYVSVVYAIN
EPAAAGAYEALRSIGRDKDVLVVAVDGGCPGVRNVKDGVIAATAMQFPLAMAQMGVEAVLKYATDGTKPKPADGQDFVNT
GVELVTDRPVPGLPSIDTDAALKKCWG

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=295, Percent_Identity=33.2203389830508, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790526, Length=299, Percent_Identity=29.4314381270903, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790674, Length=263, Percent_Identity=29.6577946768061, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35928; Mature: 35928

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLWPRLLMAASVAWAGLAATSPAGAAEKPADKPIVGIVIKTSTNPFFVKMKEGAEAAAA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHH
RLGLELRSFAGKMDGDTDAQVTAVESLIAAGAKGILITPSDSRAIVPVLEQARAKGILVI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCEEEEE
ALDTPLDPPGAADATFATDNVLAGKLVGEWAAKTLGANAKSAKVALLDLNVNQISVDVDR
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECC
DQGFLKGFGVNVADPAVIGDEKDSRIVGHDVTLGNEEGGRRAMENLLQRDPYVSVVYAIN
CCCCHHCCCCCCCCCCEECCCCCCEEEECEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
EPAAAGAYEALRSIGRDKDVLVVAVDGGCPGVRNVKDGVIAATAMQFPLAMAQMGVEAVL
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KYATDGTKPKPADGQDFVNTGVELVTDRPVPGLPSIDTDAALKKCWG
HHHCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHLWPRLLMAASVAWAGLAATSPAGAAEKPADKPIVGIVIKTSTNPFFVKMKEGAEAAAA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCHHHHH
RLGLELRSFAGKMDGDTDAQVTAVESLIAAGAKGILITPSDSRAIVPVLEQARAKGILVI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCEEEEE
ALDTPLDPPGAADATFATDNVLAGKLVGEWAAKTLGANAKSAKVALLDLNVNQISVDVDR
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECC
DQGFLKGFGVNVADPAVIGDEKDSRIVGHDVTLGNEEGGRRAMENLLQRDPYVSVVYAIN
CCCCHHCCCCCCCCCCEECCCCCCEEEECEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
EPAAAGAYEALRSIGRDKDVLVVAVDGGCPGVRNVKDGVIAATAMQFPLAMAQMGVEAVL
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KYATDGTKPKPADGQDFVNTGVELVTDRPVPGLPSIDTDAALKKCWG
HHHCCCCCCCCCCCHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]