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The map label for this gene is purF [H]

Identifier: 154246341

GI number: 154246341

Start: 2677801

End: 2679345

Strand: Reverse

Name: purF [H]

Synonym: Xaut_2400

Alternate gene names: 154246341

Gene position: 2679345-2677801 (Counterclockwise)

Preceding gene: 154246342

Following gene: 154246340

Centisome position: 50.47

GC content: 68.35

Gene sequence:

>1545_bases
ATGGATTTCGACAGTGACGCCCCGATGACCGCGGGCGACGACCCCCGTTCCGAGGATGGCCATCCCCGGCTGGCCGGGCT
GACCCAGGCCGAACAGGCGGATCTCGACCTGAACGGTGACCGACTCCGCGAGGAATGCGGTGTCTTCGGCATCTACAATC
ACCCGGAGGCGGCGGCCATCACCGCCATCGGGCTGCATGCGCTCCAGCATCGCGGGCAGGAGGCGGCCGGCATCGTCTCC
TACGACGGCAAGCGCTTCCATTCGGAGCGGCGCCTCGGCCTCGTGGGCGACGCCTTCTCCGACGCCAGCGTCATCGAGCG
CCTGCCCGGCGACATGGCGGTGGGCCATGTGCGCTATTCCACCACCGGCGAGACCCTGCTGCGCAACGTGCAGCCCCTGT
TCGCCGAGCTCGACGCCGGCGGCTTCGCCGTGGGCCACAACGGCAATCTCACGAACGGCCTCACCCTGCGCAAGCAGCTG
GTGCGGGACGGGGCCATCACCCAGTCCACCACCGACACCGAGGCCATCCTCCATCTGGTCGCCCGCTCCAAGAAGCCGCG
CTTCATCGACCGCTTCACCGACGCCCTGCGCGCGCTGGAGGGGGCCTATTCCCTGGTGGCCCTCACCAACAAGAAGCTCA
TCGGCGCCCGCGACCCCCTCGGCATCCGCCCGCTTGTGCTGGGCATGCTGGACGGCTCGCCCATCCTCGCCTCCGAGACC
TGCGCCCTCGACATCATCGGCGCGCGCTATGTGCGCGACGTGGAGAACGGCGAGGTCATCGTGATCGACGAGGACGGCCT
GCAGTCGTTCAAGCCGTTCCCGGAGATGGCGCCGCGGCCCTGCATCTTCGAGTACATCTATTTCGCCCGGCCCGATTCCG
TGGTGGGCGGGCGCTCGGTCTACCAAGTGCGCAAGAAGATGGGTCGCGTGCTGGCGGAGGAGAGCCCCGCCAATGCCGAC
GTCATCGTTCCCGTGCCGGATTCCGGCGTGCCGGCGGCCATCGGCTTCTCCCAGGCCTCGGGCATCCCCTACGAGCTGGG
CATCATCCGCAACCATTATGTGGGGCGCACCTTCATCCAGCCCACCCAGTCCATCCGCGACCAGGGCGTGCGCATGAAGC
ATTCCGCCAACCGCTCGGTGGTGGAAGGCCGCTCCATCGTGCTGGTGGACGACAGCCTGGTGCGCGGCACCACCTCGGTG
AAGATCGTGCAGATGATGCGTGACGCCGGCGCCCGGGAGGTGCATTTCCGCATCGCCTCCCCGCCCATCACCCATCCCGA
CTATTACGGCATCGACACCCCGGACCGCGACAAGCTGCTGGCCGCCACCCACGACCTGGAAGGCATGCGGCGCTATATCG
GCGCGGATTCGCTGGCCTTCCTCTCGGTGGACGGCGTCTACCGCGCCATGGGCTTCGAGGGCCGCGATCCGGTGAAGCCG
CAATTCACCGACCACTGCTTCACCGGCGACTATCCCACCCCGCTCACCGACCGGGCCGGCGCCGTGGGCCAACAGCAGCT
TTCGCTCCTGGCCGAGGCGAGCTGA

Upstream 100 bases:

>100_bases
GGGGTTAAGTCCTACCGCCCCACGGAACGCCAGGGCCTCGACCAGCTCGTCGCCAGCACGCGCGGCACCGGCCAGTAAGC
CGCCCGAGCGGAGGTGCATG

Downstream 100 bases:

>100_bases
GACGCGCCTCTTGAAAAGCCCTCCCCCCAACCTGCGGGGGAGGGTGTTTTTTCCCTTCAGTCCACGGAAGCCGCATGACC
GCCGACCGGCCCCTCGCCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDFDSDAPMTAGDDPRSEDGHPRLAGLTQAEQADLDLNGDRLREECGVFGIYNHPEAAAITAIGLHALQHRGQEAAGIVS
YDGKRFHSERRLGLVGDAFSDASVIERLPGDMAVGHVRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQL
VRDGAITQSTTDTEAILHLVARSKKPRFIDRFTDALRALEGAYSLVALTNKKLIGARDPLGIRPLVLGMLDGSPILASET
CALDIIGARYVRDVENGEVIVIDEDGLQSFKPFPEMAPRPCIFEYIYFARPDSVVGGRSVYQVRKKMGRVLAEESPANAD
VIVPVPDSGVPAAIGFSQASGIPYELGIIRNHYVGRTFIQPTQSIRDQGVRMKHSANRSVVEGRSIVLVDDSLVRGTTSV
KIVQMMRDAGAREVHFRIASPPITHPDYYGIDTPDRDKLLAATHDLEGMRRYIGADSLAFLSVDGVYRAMGFEGRDPVKP
QFTDHCFTGDYPTPLTDRAGAVGQQQLSLLAEAS

Sequences:

>Translated_514_residues
MDFDSDAPMTAGDDPRSEDGHPRLAGLTQAEQADLDLNGDRLREECGVFGIYNHPEAAAITAIGLHALQHRGQEAAGIVS
YDGKRFHSERRLGLVGDAFSDASVIERLPGDMAVGHVRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQL
VRDGAITQSTTDTEAILHLVARSKKPRFIDRFTDALRALEGAYSLVALTNKKLIGARDPLGIRPLVLGMLDGSPILASET
CALDIIGARYVRDVENGEVIVIDEDGLQSFKPFPEMAPRPCIFEYIYFARPDSVVGGRSVYQVRKKMGRVLAEESPANAD
VIVPVPDSGVPAAIGFSQASGIPYELGIIRNHYVGRTFIQPTQSIRDQGVRMKHSANRSVVEGRSIVLVDDSLVRGTTSV
KIVQMMRDAGAREVHFRIASPPITHPDYYGIDTPDRDKLLAATHDLEGMRRYIGADSLAFLSVDGVYRAMGFEGRDPVKP
QFTDHCFTGDYPTPLTDRAGAVGQQQLSLLAEAS
>Mature_514_residues
MDFDSDAPMTAGDDPRSEDGHPRLAGLTQAEQADLDLNGDRLREECGVFGIYNHPEAAAITAIGLHALQHRGQEAAGIVS
YDGKRFHSERRLGLVGDAFSDASVIERLPGDMAVGHVRYSTTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQL
VRDGAITQSTTDTEAILHLVARSKKPRFIDRFTDALRALEGAYSLVALTNKKLIGARDPLGIRPLVLGMLDGSPILASET
CALDIIGARYVRDVENGEVIVIDEDGLQSFKPFPEMAPRPCIFEYIYFARPDSVVGGRSVYQVRKKMGRVLAEESPANAD
VIVPVPDSGVPAAIGFSQASGIPYELGIIRNHYVGRTFIQPTQSIRDQGVRMKHSANRSVVEGRSIVLVDDSLVRGTTSV
KIVQMMRDAGAREVHFRIASPPITHPDYYGIDTPDRDKLLAATHDLEGMRRYIGADSLAFLSVDGVYRAMGFEGRDPVKP
QFTDHCFTGDYPTPLTDRAGAVGQQQLSLLAEAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=469, Percent_Identity=37.5266524520256, Blast_Score=331, Evalue=9e-91,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=38.4453781512605, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=30.7359307359307, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=38.2045929018789, Blast_Score=306, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=481, Percent_Identity=34.7193347193347, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=39.2276422764228, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI28573187, Length=511, Percent_Identity=38.9432485322896, Blast_Score=338, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24659604, Length=471, Percent_Identity=39.4904458598726, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55834; Mature: 55834

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDSDAPMTAGDDPRSEDGHPRLAGLTQAEQADLDLNGDRLREECGVFGIYNHPEAAAI
CCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHCCCCCCHHHHHHHCCEEEECCCCCHHHH
TAIGLHALQHRGQEAAGIVSYDGKRFHSERRLGLVGDAFSDASVIERLPGDMAVGHVRYS
HHHHHHHHHHCCCCCCEEEECCCHHHHCCCCCCEECCCCCCHHHHHHCCCCEEEEEEEEC
TTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQLVRDGAITQSTTDTEAILHLV
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
ARSKKPRFIDRFTDALRALEGAYSLVALTNKKLIGARDPLGIRPLVLGMLDGSPILASET
HHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCCCCHHEEEEECCCCEEECCC
CALDIIGARYVRDVENGEVIVIDEDGLQSFKPFPEMAPRPCIFEYIYFARPDSVVGGRSV
CEEEEHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHH
YQVRKKMGRVLAEESPANADVIVPVPDSGVPAAIGFSQASGIPYELGIIRNHYVGRTFIQ
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCHHCCCCCEEEEEECCCCCCCHHCC
PTQSIRDQGVRMKHSANRSVVEGRSIVLVDDSLVRGTTSVKIVQMMRDAGAREVHFRIAS
CHHHHHHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECC
PPITHPDYYGIDTPDRDKLLAATHDLEGMRRYIGADSLAFLSVDGVYRAMGFEGRDPVKP
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCC
QFTDHCFTGDYPTPLTDRAGAVGQQQLSLLAEAS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MDFDSDAPMTAGDDPRSEDGHPRLAGLTQAEQADLDLNGDRLREECGVFGIYNHPEAAAI
CCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHCCCCCCHHHHHHHCCEEEECCCCCHHHH
TAIGLHALQHRGQEAAGIVSYDGKRFHSERRLGLVGDAFSDASVIERLPGDMAVGHVRYS
HHHHHHHHHHCCCCCCEEEECCCHHHHCCCCCCEECCCCCCHHHHHHCCCCEEEEEEEEC
TTGETLLRNVQPLFAELDAGGFAVGHNGNLTNGLTLRKQLVRDGAITQSTTDTEAILHLV
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
ARSKKPRFIDRFTDALRALEGAYSLVALTNKKLIGARDPLGIRPLVLGMLDGSPILASET
HHCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCCCCHHEEEEECCCCEEECCC
CALDIIGARYVRDVENGEVIVIDEDGLQSFKPFPEMAPRPCIFEYIYFARPDSVVGGRSV
CEEEEHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHH
YQVRKKMGRVLAEESPANADVIVPVPDSGVPAAIGFSQASGIPYELGIIRNHYVGRTFIQ
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEECCHHCCCCCEEEEEECCCCCCCHHCC
PTQSIRDQGVRMKHSANRSVVEGRSIVLVDDSLVRGTTSVKIVQMMRDAGAREVHFRIAS
CHHHHHHCCCEEECCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEEEECC
PPITHPDYYGIDTPDRDKLLAATHDLEGMRRYIGADSLAFLSVDGVYRAMGFEGRDPVKP
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCC
QFTDHCFTGDYPTPLTDRAGAVGQQQLSLLAEAS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA