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The map label for this gene is artI [H]

Identifier: 154246325

GI number: 154246325

Start: 2661406

End: 2662359

Strand: Direct

Name: artI [H]

Synonym: Xaut_2383

Alternate gene names: 154246325

Gene position: 2661406-2662359 (Clockwise)

Preceding gene: 154246323

Following gene: 154246326

Centisome position: 50.13

GC content: 69.5

Gene sequence:

>954_bases
ATGGCTCTACCAGTCCTTATCGCCCGCCTGCGGGCCGTGGTCGCGGGTCGTGGCTTGAGGCGTCGCCTTTGCGTCGCGCT
CGCGCTGGCGGCCCTGCCCTGGGTCGCGCAGGCCCAGCAGCCGCCGGGGCCGTCGCCCGTGTGGGGTCCGGTCCGGGGCG
GGCAGGTGAGCGGTGGGGGCGCCGGCGGCGTATCGGTGCCCAATTTCTGGAATGTGGAGCGACGGCTGGAGCGGCCTGAT
GCCGCGCGCCTGCCCAAGGAACTGCGCTTCGTTACCACCGACGACTACCCGCCCTTCAACTTCCTGAATGCCGATGGCGC
CTTGACCGGCTTCAACGTGGACCTTGCCCGCGCCATCTGTGCCGAGCTGGGGGTGACCTGCGTGATCCAGGCGGCAGCCT
TCGACACGCTGGCCGACAGCGTCGAGCAGGGCCGGGCGGACGCGGCCATCGCCGGCCTCACCGCCACGCCCCAGTCCCGC
GCCGCGCTGGATTTCTCCGAGCGCTATCTCGGCACCCCGGCGCGCTTCGTGGGCAAAAGGCAGGAGACGCTCGCCGCCGC
CACCCCGGAGAATGTGGCGGCCAAGAAGGTGGCGGTGGTCGCCCGCACCGCCCACGAGGCCTATCTGCGTTCCTTCTTCA
ACGAAGCCGCCATCCGCCCCTATCCGGACCTGAAGGCGGCCCAGGAGGCGCTGAAGGCAGGCCAGGTGGACCTGCTGTTC
GGCGACGGGATCACGCTCGCCCTGTGGCTCAATGGCGCCGACAGCGCCAATTGCTGCCGCTTCCTGGGCGGCCCCTTCAC
CGAGAGCCGCTTCTTTGGCGACGGCTTCTCCGTGGCGGTGAAGGAGGGCAACGACACCCTGCGCCACGCCATGGACTTCG
CCCTGCAGCGCATCTGGGAAAAGGGCGTCTATACCGACCTTTACCTGCGCTGGTTCCCCATCGGCTTCTATTGA

Upstream 100 bases:

>100_bases
GCTCCTCGGCCCGCCGCCCGGCCCACCCCTTGTCGGCGGACCGCGCATCCCCCACCATGCGCCGCCCCTTCGAGTGCAAC
CCTAAGGTAACCGCGCCGAC

Downstream 100 bases:

>100_bases
GGAGCCATCGGGTCCCGTCGCGGACGGTGTTTCGGCCCGCCCGCGTTGACGCCACCCGCCGACATTCCTAAGTTCGCGCT
CCTTTTTTGGAGCCTGCCAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MALPVLIARLRAVVAGRGLRRRLCVALALAALPWVAQAQQPPGPSPVWGPVRGGQVSGGGAGGVSVPNFWNVERRLERPD
AARLPKELRFVTTDDYPPFNFLNADGALTGFNVDLARAICAELGVTCVIQAAAFDTLADSVEQGRADAAIAGLTATPQSR
AALDFSERYLGTPARFVGKRQETLAAATPENVAAKKVAVVARTAHEAYLRSFFNEAAIRPYPDLKAAQEALKAGQVDLLF
GDGITLALWLNGADSANCCRFLGGPFTESRFFGDGFSVAVKEGNDTLRHAMDFALQRIWEKGVYTDLYLRWFPIGFY

Sequences:

>Translated_317_residues
MALPVLIARLRAVVAGRGLRRRLCVALALAALPWVAQAQQPPGPSPVWGPVRGGQVSGGGAGGVSVPNFWNVERRLERPD
AARLPKELRFVTTDDYPPFNFLNADGALTGFNVDLARAICAELGVTCVIQAAAFDTLADSVEQGRADAAIAGLTATPQSR
AALDFSERYLGTPARFVGKRQETLAAATPENVAAKKVAVVARTAHEAYLRSFFNEAAIRPYPDLKAAQEALKAGQVDLLF
GDGITLALWLNGADSANCCRFLGGPFTESRFFGDGFSVAVKEGNDTLRHAMDFALQRIWEKGVYTDLYLRWFPIGFY
>Mature_316_residues
ALPVLIARLRAVVAGRGLRRRLCVALALAALPWVAQAQQPPGPSPVWGPVRGGQVSGGGAGGVSVPNFWNVERRLERPDA
ARLPKELRFVTTDDYPPFNFLNADGALTGFNVDLARAICAELGVTCVIQAAAFDTLADSVEQGRADAAIAGLTATPQSRA
ALDFSERYLGTPARFVGKRQETLAAATPENVAAKKVAVVARTAHEAYLRSFFNEAAIRPYPDLKAAQEALKAGQVDLLFG
DGITLALWLNGADSANCCRFLGGPFTESRFFGDGFSVAVKEGNDTLRHAMDFALQRIWEKGVYTDLYLRWFPIGFY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=31.4655172413793, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787085, Length=228, Percent_Identity=27.1929824561404, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788649, Length=238, Percent_Identity=24.3697478991597, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=23.3050847457627, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788228, Length=217, Percent_Identity=27.1889400921659, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787031, Length=227, Percent_Identity=25.5506607929515, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 34157; Mature: 34026

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPVLIARLRAVVAGRGLRRRLCVALALAALPWVAQAQQPPGPSPVWGPVRGGQVSGGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCC
AGGVSVPNFWNVERRLERPDAARLPKELRFVTTDDYPPFNFLNADGALTGFNVDLARAIC
CCCCCCCCCCCHHHHHCCCCHHCCHHHCEEEECCCCCCCCEECCCCCEECCCHHHHHHHH
AELGVTCVIQAAAFDTLADSVEQGRADAAIAGLTATPQSRAALDFSERYLGTPARFVGKR
HHCCHHEEHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHCCHHHHHCCCHHHHHCCH
QETLAAATPENVAAKKVAVVARTAHEAYLRSFFNEAAIRPYPDLKAAQEALKAGQVDLLF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEE
GDGITLALWLNGADSANCCRFLGGPFTESRFFGDGFSVAVKEGNDTLRHAMDFALQRIWE
CCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
KGVYTDLYLRWFPIGFY
CCCHHEEEEEEEECCCC
>Mature Secondary Structure 
ALPVLIARLRAVVAGRGLRRRLCVALALAALPWVAQAQQPPGPSPVWGPVRGGQVSGGG
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCC
AGGVSVPNFWNVERRLERPDAARLPKELRFVTTDDYPPFNFLNADGALTGFNVDLARAIC
CCCCCCCCCCCHHHHHCCCCHHCCHHHCEEEECCCCCCCCEECCCCCEECCCHHHHHHHH
AELGVTCVIQAAAFDTLADSVEQGRADAAIAGLTATPQSRAALDFSERYLGTPARFVGKR
HHCCHHEEHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHCCHHHHHCCCHHHHHCCH
QETLAAATPENVAAKKVAVVARTAHEAYLRSFFNEAAIRPYPDLKAAQEALKAGQVDLLF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEE
GDGITLALWLNGADSANCCRFLGGPFTESRFFGDGFSVAVKEGNDTLRHAMDFALQRIWE
CCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
KGVYTDLYLRWFPIGFY
CCCHHEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]