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The map label for this gene is bepF [H]

Identifier: 154246301

GI number: 154246301

Start: 2631883

End: 2633061

Strand: Direct

Name: bepF [H]

Synonym: Xaut_2359

Alternate gene names: 154246301

Gene position: 2631883-2633061 (Clockwise)

Preceding gene: 154246299

Following gene: 154246302

Centisome position: 49.57

GC content: 68.28

Gene sequence:

>1179_bases
ATGACGCTTGCCCCGCGCCTTTGGGTTGTTCTTGCTGCGTTTGCCCTGCCGCTTGCCGCCTGCGGACAGCCTTCCGCCCA
GCAGCAGGGCGCGCCGCCGCCGCCCCAGGTGACGGTGGCCACGCCGCAGACCAAGACCGTCACCGACCAGGACGAATATG
TCGGCCGCTTCGTCGCCATCGACGAGGTGAACGTGCGTGCCCGGGTGGCCGGCTATCTGGACAAGATCCATTTCACCGAC
GGCCAGATGGTGAAGGAGGGCGACCTGCTCTTCACCATCGACCAGCGCCCCTACCTGATCGCCCTCGACCAGGCCAAGGC
GAACCTGGACCAAGCCAAGGCCAATCTCGACTTCACCAAGGCCGACCTCGACCGCGCCAAGACGCTGCTGGAGGACCGCA
CCTCCAACGCCATCTCCAAGCAGACGTTCGACCAGCGCACCCAGGCCGCGCGCTCCGCCGCCGCGACCGTGCTGTCGCAG
GAGGCCGCCGTCCACTCGGCCCAGCTCGACCTCACCTTCACCGAGGTGCGCGCCCCGGTCTCGGGCCGCATTGGCGATCG
GCGCGTGTCGGTGGGCAATTACGTGGCGGCCGGCTCCGGCGCCACGCCCACCCTTCTCGCGGTGATCGTCTCCATCGATC
CCATCCGCTTCGAATTCACCATCGACGAGGCCTCCCTGCTGCGCATCCTGCGCCGCAAGGGCGGGGCCGACGTGTCCGGC
GACCCGGTCGCCCTCAAGCTGATCGACGACAAGGATTTCATCCACCCGGGCAAGCTCGACTTCCTGAACAACGTCATCGA
CCGGGAAACCGGCACCATCCGTGGCCGCGCGGTGTTCGAGAACAAGAACGGCCTGTTCCGCCCCGGCATGTTCGCCCGTA
TCCGCCTCGATTCCTCCGCCCCCTACCAGGCGCTGGCCGTGCCGGACGTCGCCATCGGCACCGAGCAGGTGAACAAGTTC
GTCTATGTGATCGGGCCCGAGAACAAGGTGCTGCAGAAGGTGGTCACCCTGGGGCCGCTGGTGGGCGACATGCGGGTGAT
CCGCTCCGGCCTCACGGCGGACGACAAGGTGGTCGTCAACGGCCTGATGCGCGTGCGGCCCGGCGTCACCGTCAACCCGC
AGATGGCGGAGGCCAGCCCTCCGCCCGCAGCCGCCCCTGCCGCCACCACCGCGAAGTGA

Upstream 100 bases:

>100_bases
ATGCGCCGTCTTCCGGTTTCGGCTCGTCGGTTGCGGGGGGTGCCGAACCTTCTTCACATCTTCGTTGGCGGGCGTTCGTC
CGTCGAGGAGCCGGATTGCC

Downstream 100 bases:

>100_bases
GGTGAGAAGATGCGCTTCTCACACTTCTTCATCGACCGGCCCATCTTCGCCTCGGTGGTGTCCGCCGTCGTCATGATCCT
TGGCGCGGTGACCTACCTGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTLAPRLWVVLAAFALPLAACGQPSAQQQGAPPPPQVTVATPQTKTVTDQDEYVGRFVAIDEVNVRARVAGYLDKIHFTD
GQMVKEGDLLFTIDQRPYLIALDQAKANLDQAKANLDFTKADLDRAKTLLEDRTSNAISKQTFDQRTQAARSAAATVLSQ
EAAVHSAQLDLTFTEVRAPVSGRIGDRRVSVGNYVAAGSGATPTLLAVIVSIDPIRFEFTIDEASLLRILRRKGGADVSG
DPVALKLIDDKDFIHPGKLDFLNNVIDRETGTIRGRAVFENKNGLFRPGMFARIRLDSSAPYQALAVPDVAIGTEQVNKF
VYVIGPENKVLQKVVTLGPLVGDMRVIRSGLTADDKVVVNGLMRVRPGVTVNPQMAEASPPPAAAPAATTAK

Sequences:

>Translated_392_residues
MTLAPRLWVVLAAFALPLAACGQPSAQQQGAPPPPQVTVATPQTKTVTDQDEYVGRFVAIDEVNVRARVAGYLDKIHFTD
GQMVKEGDLLFTIDQRPYLIALDQAKANLDQAKANLDFTKADLDRAKTLLEDRTSNAISKQTFDQRTQAARSAAATVLSQ
EAAVHSAQLDLTFTEVRAPVSGRIGDRRVSVGNYVAAGSGATPTLLAVIVSIDPIRFEFTIDEASLLRILRRKGGADVSG
DPVALKLIDDKDFIHPGKLDFLNNVIDRETGTIRGRAVFENKNGLFRPGMFARIRLDSSAPYQALAVPDVAIGTEQVNKF
VYVIGPENKVLQKVVTLGPLVGDMRVIRSGLTADDKVVVNGLMRVRPGVTVNPQMAEASPPPAAAPAATTAK
>Mature_391_residues
TLAPRLWVVLAAFALPLAACGQPSAQQQGAPPPPQVTVATPQTKTVTDQDEYVGRFVAIDEVNVRARVAGYLDKIHFTDG
QMVKEGDLLFTIDQRPYLIALDQAKANLDQAKANLDFTKADLDRAKTLLEDRTSNAISKQTFDQRTQAARSAAATVLSQE
AAVHSAQLDLTFTEVRAPVSGRIGDRRVSVGNYVAAGSGATPTLLAVIVSIDPIRFEFTIDEASLLRILRRKGGADVSGD
PVALKLIDDKDFIHPGKLDFLNNVIDRETGTIRGRAVFENKNGLFRPGMFARIRLDSSAPYQALAVPDVAIGTEQVNKFV
YVIGPENKVLQKVVTLGPLVGDMRVIRSGLTADDKVVVNGLMRVRPGVTVNPQMAEASPPPAAAPAATTAK

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=331, Percent_Identity=33.8368580060423, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786668, Length=391, Percent_Identity=32.4808184143223, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789929, Length=369, Percent_Identity=29.810298102981, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=30.4812834224599, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081951, Length=138, Percent_Identity=31.8840579710145, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789637, Length=151, Percent_Identity=32.4503311258278, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42130; Mature: 41998

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAPRLWVVLAAFALPLAACGQPSAQQQGAPPPPQVTVATPQTKTVTDQDEYVGRFVAI
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHCEEEEEE
DEVNVRARVAGYLDKIHFTDGQMVKEGDLLFTIDQRPYLIALDQAKANLDQAKANLDFTK
ECCCHHHHHHHHHHHEECCCCCEEECCCEEEEECCCCEEEEECCHHCCHHHHHCCCCCHH
ADLDRAKTLLEDRTSNAISKQTFDQRTQAARSAAATVLSQEAAVHSAQLDLTFTEVRAPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHCCCC
SGRIGDRRVSVGNYVAAGSGATPTLLAVIVSIDPIRFEFTIDEASLLRILRRKGGADVSG
CCCCCCEEEECCCEEEECCCCCHHHHHHEEECCCEEEEEEECHHHHHHHHHHCCCCCCCC
DPVALKLIDDKDFIHPGKLDFLNNVIDRETGTIRGRAVFENKNGLFRPGMFARIRLDSSA
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCC
PYQALAVPDVAIGTEQVNKFVYVIGPENKVLQKVVTLGPLVGDMRVIRSGLTADDKVVVN
CEEEEECCCCCCCHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHH
GLMRVRPGVTVNPQMAEASPPPAAAPAATTAK
HHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLAPRLWVVLAAFALPLAACGQPSAQQQGAPPPPQVTVATPQTKTVTDQDEYVGRFVAI
CCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHCEEEEEE
DEVNVRARVAGYLDKIHFTDGQMVKEGDLLFTIDQRPYLIALDQAKANLDQAKANLDFTK
ECCCHHHHHHHHHHHEECCCCCEEECCCEEEEECCCCEEEEECCHHCCHHHHHCCCCCHH
ADLDRAKTLLEDRTSNAISKQTFDQRTQAARSAAATVLSQEAAVHSAQLDLTFTEVRAPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHCCCC
SGRIGDRRVSVGNYVAAGSGATPTLLAVIVSIDPIRFEFTIDEASLLRILRRKGGADVSG
CCCCCCEEEECCCEEEECCCCCHHHHHHEEECCCEEEEEEECHHHHHHHHHHCCCCCCCC
DPVALKLIDDKDFIHPGKLDFLNNVIDRETGTIRGRAVFENKNGLFRPGMFARIRLDSSA
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCC
PYQALAVPDVAIGTEQVNKFVYVIGPENKVLQKVVTLGPLVGDMRVIRSGLTADDKVVVN
CEEEEECCCCCCCHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHH
GLMRVRPGVTVNPQMAEASPPPAAAPAATTAK
HHHHHCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]