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The map label for this gene is rnhA [H]

Identifier: 154246299

GI number: 154246299

Start: 2630737

End: 2631192

Strand: Direct

Name: rnhA [H]

Synonym: Xaut_2357

Alternate gene names: 154246299

Gene position: 2630737-2631192 (Clockwise)

Preceding gene: 154246298

Following gene: 154246301

Centisome position: 49.55

GC content: 68.42

Gene sequence:

>456_bases
ATGAAAGAAGTGGCGGTGTTCACGGACGGGGCCTGCTCGGGCAATCCCGGCCCCGGCGGCTGGGGCGCGATCCTGCGCTT
CGGCGCCCACGAGAAGGAGCTGAGCGGTGGCGAGGCCCTCACCACCAACAACCGCATGGAGCTGATGGGCGCCATCGCCG
CGCTGGAGGCGCTGAAGGAGCCGTGCACGGTGGATCTGCACACCGACAGCAACTACCTGAAGGACGGCGTGACCAAGTGG
ATGCACGGCTGGAAGCGCAACGGCTGGCGCACCGCCGACAAGAAGCCGGTGAAGAACCAGGACCTGTGGGAGCGCCTCGA
TGCGGCGCTGAAGCGCCACACCCTGCGCTGGCATTGGGTGAAGGGCCATGCCGGCCACGCTGAGAACGAGCGCGCGGACG
AACTGGCCCGCGCCGGCATGGCCCCGTTCAAGCTCAAGGGCCGACTCAGCGGCTGA

Upstream 100 bases:

>100_bases
CCGAGGGCGCTTTGGTGCGCCCCAAGGACCCGCTGGAATATCTGAAGAAGCTGCGCTTCCACCAGGCGGTGGACAGCGCG
CAGGATTACGGTCTGGCGGC

Downstream 100 bases:

>100_bases
GGGCGCGCGGCGCCGGCCGCCGGAGCTATTTCTTCTGGGCCGCCGCTTCCTGTTTCTTGCGCTCAATGCAGCGCGAGATG
AGGGTCGAGCGGTCCGCCTG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKEVAVFTDGACSGNPGPGGWGAILRFGAHEKELSGGEALTTNNRMELMGAIAALEALKEPCTVDLHTDSNYLKDGVTKW
MHGWKRNGWRTADKKPVKNQDLWERLDAALKRHTLRWHWVKGHAGHAENERADELARAGMAPFKLKGRLSG

Sequences:

>Translated_151_residues
MKEVAVFTDGACSGNPGPGGWGAILRFGAHEKELSGGEALTTNNRMELMGAIAALEALKEPCTVDLHTDSNYLKDGVTKW
MHGWKRNGWRTADKKPVKNQDLWERLDAALKRHTLRWHWVKGHAGHAENERADELARAGMAPFKLKGRLSG
>Mature_151_residues
MKEVAVFTDGACSGNPGPGGWGAILRFGAHEKELSGGEALTTNNRMELMGAIAALEALKEPCTVDLHTDSNYLKDGVTKW
MHGWKRNGWRTADKKPVKNQDLWERLDAALKRHTLRWHWVKGHAGHAENERADELARAGMAPFKLKGRLSG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=37.4149659863946, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=67.1328671328671, Blast_Score=207, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI115532542, Length=144, Percent_Identity=38.8888888888889, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=37.1212121212121, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137476, Length=153, Percent_Identity=33.9869281045752, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552509, Length=153, Percent_Identity=33.9869281045752, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16712; Mature: 16712

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVAVFTDGACSGNPGPGGWGAILRFGAHEKELSGGEALTTNNRMELMGAIAALEALKE
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHCC
PCTVDLHTDSNYLKDGVTKWMHGWKRNGWRTADKKPVKNQDLWERLDAALKRHTLRWHWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGHAGHAENERADELARAGMAPFKLKGRLSG
CCCCCCCCCHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MKEVAVFTDGACSGNPGPGGWGAILRFGAHEKELSGGEALTTNNRMELMGAIAALEALKE
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHCC
PCTVDLHTDSNYLKDGVTKWMHGWKRNGWRTADKKPVKNQDLWERLDAALKRHTLRWHWV
CCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGHAGHAENERADELARAGMAPFKLKGRLSG
CCCCCCCCCHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA