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The map label for this gene is hslU

Identifier: 154246238

GI number: 154246238

Start: 2559579

End: 2560886

Strand: Direct

Name: hslU

Synonym: Xaut_2296

Alternate gene names: 154246238

Gene position: 2559579-2560886 (Clockwise)

Preceding gene: 154246237

Following gene: 154246241

Centisome position: 48.21

GC content: 68.96

Gene sequence:

>1308_bases
ATGAGCGACTTTTCCCCCCGCGAGATCGTCTCCGAGCTGGACCGCCACATCGTCGGCCAGGCCAATGCGAAGAGGGCGGT
GGCTATCGCCCTGCGCAACCGGTGGCGGCGCCAGCAGCTGGACGACAAGCTGCGCGAGGAGGTGCTGCCCAAGAACATCC
TCATGATCGGCCCCACGGGCGTCGGCAAGACCGAGATTTCCCGCCGCCTCGCCCGGCTTGCCGGCGCGCCCTTCCTCAAG
GTCGAGGCCACCAAGTTCACGGAAGTGGGCTACGTGGGCCGCGACGTGGAGCAGATCGTGCGCGACCTCGTGGAGGTGGG
CATCGGCCTGGTGCGCGAGGCGCGGCGCAAGGATGTGCAGGCCAAGGCCCATCTCGCCGCCGAGAACCGGGTGCTCGACG
CCCTGGTGGGCGCCACCGCCTCCCCCGCCACCCGCGACGCCTTCCGCAAGCGCCTGCGCGCGGGCGAGCTGGACGACAAG
GAAGTGGAGATCGAGGTGCAGGCCGGCGGGCAGGGCATGCCCATGTTCGAGATTCCCGGCATGCCCGGCACCCAGATGGG
CGCCGTCAATCTGGGCGACATGCTGGGCAAGGCCTTCGGTGGCCGCTCCAAGCCGCGCCGCGTCAATGTGAAGGAGGCCC
ATCCCCTCCTGCTCGCCGAGGAGGCGGACAAGCTCATCGACCAGGACGCCATCGTCCAGGATGCGATCCACCAGGTGGAG
AACAACGGCATCGTCTTCCTCGACGAGATCGACAAGATCGCTGGCCGCGAGGGCCGCTCGGGCGCGGATGTCTCCCGCGA
GGGGGTGCAGCGCGACCTGCTCCCCCTCATCGAGGGCACCACGGTTTCCACCAAGCACGGGCCGGTTAAGACCGACCACA
TCCTGTTCATCGCCTCCGGCGCCTTCCACGTCTCCAAGCCGTCGGACCTGCTGCCGGAGCTTCAGGGCCGCCTGCCCATC
CGCGTGGAGCTGGAGGCGCTGACCCGCGCCGACTTCGTGCGCATCCTCACCGAGACCGAGGCGAGCCTGGTCAAGCAGTC
GGTGGCGCTCATGGGCACCGAGGGCGTGACCCTCACCATCACCACCGACGCGGTGGAGGCCATCGCCGACGCGGCGGTGG
AGGTGAATTCCAATGTGGAGAACATCGGCGCCCGGCGGCTCCAGACCGTCATCGAGCGGGTGCTGGACGACCTGTCCTTC
TCCGCGCCCGACCGCTCCGGCGAGACGGTGGTGGTGGATGCGGAATATGTGCGCTCCCGCGTCGCCGACCTTGCCCGCAA
CGCCGACCTGAGCCGCTTCATCCTCTGA

Upstream 100 bases:

>100_bases
ACATCGTGGTGGAAACCATCGGCGCCTGAGGCCCCGCCCGCGCCCCGGCGGCGCGAGCGACCGCGACCCGAACGCCACCA
GATTCCAAGAGCGACCCTTG

Downstream 100 bases:

>100_bases
GGCGGGCAACTCCGAGGCAGCGATCCTCTGGCAGGATCGTCTCACGCAGGCGTCGGCCTCTCTCTCACGAGAGCCGGCGC
CGGTCCTGCCAGGCGAGTTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSDFSPREIVSELDRHIVGQANAKRAVAIALRNRWRRQQLDDKLREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLK
VEATKFTEVGYVGRDVEQIVRDLVEVGIGLVREARRKDVQAKAHLAAENRVLDALVGATASPATRDAFRKRLRAGELDDK
EVEIEVQAGGQGMPMFEIPGMPGTQMGAVNLGDMLGKAFGGRSKPRRVNVKEAHPLLLAEEADKLIDQDAIVQDAIHQVE
NNGIVFLDEIDKIAGREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPI
RVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLTITTDAVEAIADAAVEVNSNVENIGARRLQTVIERVLDDLSF
SAPDRSGETVVVDAEYVRSRVADLARNADLSRFIL

Sequences:

>Translated_435_residues
MSDFSPREIVSELDRHIVGQANAKRAVAIALRNRWRRQQLDDKLREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLK
VEATKFTEVGYVGRDVEQIVRDLVEVGIGLVREARRKDVQAKAHLAAENRVLDALVGATASPATRDAFRKRLRAGELDDK
EVEIEVQAGGQGMPMFEIPGMPGTQMGAVNLGDMLGKAFGGRSKPRRVNVKEAHPLLLAEEADKLIDQDAIVQDAIHQVE
NNGIVFLDEIDKIAGREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPI
RVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLTITTDAVEAIADAAVEVNSNVENIGARRLQTVIERVLDDLSF
SAPDRSGETVVVDAEYVRSRVADLARNADLSRFIL
>Mature_434_residues
SDFSPREIVSELDRHIVGQANAKRAVAIALRNRWRRQQLDDKLREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLKV
EATKFTEVGYVGRDVEQIVRDLVEVGIGLVREARRKDVQAKAHLAAENRVLDALVGATASPATRDAFRKRLRAGELDDKE
VEIEVQAGGQGMPMFEIPGMPGTQMGAVNLGDMLGKAFGGRSKPRRVNVKEAHPLLLAEEADKLIDQDAIVQDAIHQVEN
NGIVFLDEIDKIAGREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPIR
VELEALTRADFVRILTETEASLVKQSVALMGTEGVTLTITTDAVEAIADAAVEVNSNVENIGARRLQTVIERVLDDLSFS
APDRSGETVVVDAEYVRSRVADLARNADLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=57.847533632287, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1786642, Length=113, Percent_Identity=43.3628318584071, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71982905, Length=252, Percent_Identity=32.5396825396825, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982908, Length=252, Percent_Identity=32.5396825396825, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648291, Length=268, Percent_Identity=32.089552238806, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648289, Length=268, Percent_Identity=32.089552238806, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_XANP2 (A7IHP6)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417196.1
- ProteinModelPortal:   A7IHP6
- SMR:   A7IHP6
- STRING:   A7IHP6
- GeneID:   5420822
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2296
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   XAUT78245:XAUT_2296-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR011704
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF07724 AAA_2; PF07728 AAA_5; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 47465; Mature: 47334

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 248-248 BINDING 313-313 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFSPREIVSELDRHIVGQANAKRAVAIALRNRWRRQQLDDKLREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEISRRLARLAGAPFLKVEATKFTEVGYVGRDVEQIVRDLVEVGIGLVREARRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHLAAENRVLDALVGATASPATRDAFRKRLRAGELDDKEVEIEVQAGGQGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGTQMGAVNLGDMLGKAFGGRSKPRRVNVKEAHPLLLAEEADKLIDQDAIVQDAIHQVE
CCCCCCCCCCHHHHHHHHHCCCCCCCEECHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
NNGIVFLDEIDKIAGREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASG
CCCEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEC
AFHVSKPSDLLPELQGRLPIRVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLTI
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TTDAVEAIADAAVEVNSNVENIGARRLQTVIERVLDDLSFSAPDRSGETVVVDAEYVRSR
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHH
VADLARNADLSRFIL
HHHHHHCCCHHHHCC
>Mature Secondary Structure 
SDFSPREIVSELDRHIVGQANAKRAVAIALRNRWRRQQLDDKLREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
VGKTEISRRLARLAGAPFLKVEATKFTEVGYVGRDVEQIVRDLVEVGIGLVREARRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHLAAENRVLDALVGATASPATRDAFRKRLRAGELDDKEVEIEVQAGGQGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGTQMGAVNLGDMLGKAFGGRSKPRRVNVKEAHPLLLAEEADKLIDQDAIVQDAIHQVE
CCCCCCCCCCHHHHHHHHHCCCCCCCEECHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
NNGIVFLDEIDKIAGREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASG
CCCEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEC
AFHVSKPSDLLPELQGRLPIRVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLTI
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TTDAVEAIADAAVEVNSNVENIGARRLQTVIERVLDDLSFSAPDRSGETVVVDAEYVRSR
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHH
VADLARNADLSRFIL
HHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA