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The map label for this gene is pcpB [H]

Identifier: 154246170

GI number: 154246170

Start: 2486229

End: 2487887

Strand: Direct

Name: pcpB [H]

Synonym: Xaut_2227

Alternate gene names: 154246170

Gene position: 2486229-2487887 (Clockwise)

Preceding gene: 154246169

Following gene: 154246171

Centisome position: 46.83

GC content: 71.37

Gene sequence:

>1659_bases
ATGGGGCGGCAGGATTCGGGGCAGAATTCCGCGCAGGCGCCGGCGGATGTGATCGTGGTGGGCGCCGGGCCGGTGGGGCT
CACGCTGGCGGCGGAGCTGGCGCGCCACAAGGTGCGCTGCCGCATCCTGGAGAAGGCCGTCGCCCCGTCCCTCTATTGCC
GCGCGCTGGGCGTCACGCCCCGGACCCTGGAAGTCTATGAGGACATGGGCGTCGTCCGCGCCATGATCGACGCCGGCCTG
TGGTTCGAGGGGCTGTGCCTCGCGGTGGACGGGGCGCCGGCGCGGACCATCGCCCCCGACCTGTCGGACCTGCCTTACGG
CATGATCGGCATCCCTCAGCCGGTGACCGAGCGCATCCTCACCGCGCATCTCGCCGGCTTCGGCATCGATGTGGAGCGGG
GCGTCGCGGTGAACGGCCTCGCGCAGGCGGGAGAGAAGGTGCGGCTCGATCTTGTCGGACCGGACGGGCGGGAGGATTTC
GCCCATGCCCGCTTCGTGGTGGGATGCGACGGCGCCCACAGTTTCGTCCGCCATGCCCTCGGCATCCCGTTCGAGGGCGA
TGCCTTTCCCTATCCCTTCATGCTGGGCGACGTGCATCTCGCTTTCCCCGGCGACCTGAAGCCGGCGCGCGGCATCGCTC
TGCGCCTCATGCGCCTGCGTGAGGACACGGCGCCGGACATGTTCATCGCCATCCCGCTGCCGGAGCACGGGCGATACCGT
GTCTCCATGCTGGCGCCGGAGTATCTGGCTGCTGCGGGATCGGGCGTGGCCCACGGCATCCAGTCCGAGCGGCCGGGGCC
GTCGCTGGGGGACATCCAGGCGGTGGCCGACCGCATCGCGCCCGGGGAGATCGTCGTCGGCGACCTGCGGTGGTCGTCCC
AGTTCCGCATCTCCATGCGGCTCGCGGCCCGCTATCGCGAAGGTCAGGTGTTCCTGGCGGGGGATGCCTGCCACATCCAT
CCGCCCACCGGCGGGCAGGGGATGAACACCGGCATCCAGGATGCCTACAACCTCGCCTGGAAGCTGGCTCTGGTGATGCG
GGGCAAGGCACCCGCATCGCTCCTCGACAGCTACGAGGCCGAGCGCCGGCCGGTGGCGGAAGACGTGATCGCCCGCACCG
TCGCCGAAAGCGTGAACATCGGCGGCTCCGGTGGCGCCCGCGACCGGCTGGCCGACACTCAGATCCGGGTGTCCTATGCG
GGCCTTGCCGGTGCCGCTCTGGTGGGCGGCGCGGCGGCGCCCGAAGGACTGCCGGGCGATAGGCTGCCGGTGGCTGGCGA
TCGGGCTCCGGATGCCTTGGGCCTGAGGCGGAGGGGTGTCGGGGCGCCGTTGAGGATGTTCCAGCTGCTGCGGGGCACGG
ATTTCGTGGTTGTCGTCGCGGTGGAGACGCCCGGCGCCCTTGTGGCGGCGGAGGCCCTCGGTGCGAGGTTGGCCGAAAGC
GGCCTGCCGGTCCGGGTCGTGGCGGTCGCGCCGGCTGGGGCGAGCCTTGCCGATCCCTACAAGGTGAGCCTTGTGGCGGA
CGAGGCGGGGGCCTTTGCCGCCACCTATGGGCTTGGCGCGGGTGAGGCCGTGCTGGTGCGTCCCGATGGATATCTCGCCT
GGCGAGGCCATGCAGAGCAGGTCGTCGCTGCGCTGCGGCAGGTGGCGGGAGCGGGGTAG

Upstream 100 bases:

>100_bases
TGGGATGGCAAGGGGCCGAACAGCGCGCGTGCCTGATCCTGGCGCGGCCTTGCATGTGCGGGCCAAAAGCTGAACCCTGC
CGCAAAAGGGGGAGACGGGC

Downstream 100 bases:

>100_bases
GTTGTCGCAGGTGCTTCTGGCGCCATTTCAGGCTGCAACGAAACGTATCCGGCTTCAGGCCAAGATTTCGAGTGTGATAT
GCCGTCGAAATGGCACCAAT

Product: monooxygenase FAD-binding

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MGRQDSGQNSAQAPADVIVVGAGPVGLTLAAELARHKVRCRILEKAVAPSLYCRALGVTPRTLEVYEDMGVVRAMIDAGL
WFEGLCLAVDGAPARTIAPDLSDLPYGMIGIPQPVTERILTAHLAGFGIDVERGVAVNGLAQAGEKVRLDLVGPDGREDF
AHARFVVGCDGAHSFVRHALGIPFEGDAFPYPFMLGDVHLAFPGDLKPARGIALRLMRLREDTAPDMFIAIPLPEHGRYR
VSMLAPEYLAAAGSGVAHGIQSERPGPSLGDIQAVADRIAPGEIVVGDLRWSSQFRISMRLAARYREGQVFLAGDACHIH
PPTGGQGMNTGIQDAYNLAWKLALVMRGKAPASLLDSYEAERRPVAEDVIARTVAESVNIGGSGGARDRLADTQIRVSYA
GLAGAALVGGAAAPEGLPGDRLPVAGDRAPDALGLRRRGVGAPLRMFQLLRGTDFVVVVAVETPGALVAAEALGARLAES
GLPVRVVAVAPAGASLADPYKVSLVADEAGAFAATYGLGAGEAVLVRPDGYLAWRGHAEQVVAALRQVAGAG

Sequences:

>Translated_552_residues
MGRQDSGQNSAQAPADVIVVGAGPVGLTLAAELARHKVRCRILEKAVAPSLYCRALGVTPRTLEVYEDMGVVRAMIDAGL
WFEGLCLAVDGAPARTIAPDLSDLPYGMIGIPQPVTERILTAHLAGFGIDVERGVAVNGLAQAGEKVRLDLVGPDGREDF
AHARFVVGCDGAHSFVRHALGIPFEGDAFPYPFMLGDVHLAFPGDLKPARGIALRLMRLREDTAPDMFIAIPLPEHGRYR
VSMLAPEYLAAAGSGVAHGIQSERPGPSLGDIQAVADRIAPGEIVVGDLRWSSQFRISMRLAARYREGQVFLAGDACHIH
PPTGGQGMNTGIQDAYNLAWKLALVMRGKAPASLLDSYEAERRPVAEDVIARTVAESVNIGGSGGARDRLADTQIRVSYA
GLAGAALVGGAAAPEGLPGDRLPVAGDRAPDALGLRRRGVGAPLRMFQLLRGTDFVVVVAVETPGALVAAEALGARLAES
GLPVRVVAVAPAGASLADPYKVSLVADEAGAFAATYGLGAGEAVLVRPDGYLAWRGHAEQVVAALRQVAGAG
>Mature_551_residues
GRQDSGQNSAQAPADVIVVGAGPVGLTLAAELARHKVRCRILEKAVAPSLYCRALGVTPRTLEVYEDMGVVRAMIDAGLW
FEGLCLAVDGAPARTIAPDLSDLPYGMIGIPQPVTERILTAHLAGFGIDVERGVAVNGLAQAGEKVRLDLVGPDGREDFA
HARFVVGCDGAHSFVRHALGIPFEGDAFPYPFMLGDVHLAFPGDLKPARGIALRLMRLREDTAPDMFIAIPLPEHGRYRV
SMLAPEYLAAAGSGVAHGIQSERPGPSLGDIQAVADRIAPGEIVVGDLRWSSQFRISMRLAARYREGQVFLAGDACHIHP
PTGGQGMNTGIQDAYNLAWKLALVMRGKAPASLLDSYEAERRPVAEDVIARTVAESVNIGGSGGARDRLADTQIRVSYAG
LAGAALVGGAAAPEGLPGDRLPVAGDRAPDALGLRRRGVGAPLRMFQLLRGTDFVVVVAVETPGALVAAEALGARLAESG
LPVRVVAVAPAGASLADPYKVSLVADEAGAFAATYGLGAGEAVLVRPDGYLAWRGHAEQVVAALRQVAGAG

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=380, Percent_Identity=27.8947368421053, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 57560; Mature: 57429

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQDSGQNSAQAPADVIVVGAGPVGLTLAAELARHKVRCRILEKAVAPSLYCRALGVTP
CCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
RTLEVYEDMGVVRAMIDAGLWFEGLCLAVDGAPARTIAPDLSDLPYGMIGIPQPVTERIL
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHH
TAHLAGFGIDVERGVAVNGLAQAGEKVRLDLVGPDGREDFAHARFVVGCDGAHSFVRHAL
HHHHHHCCCCCCCCCEECHHHHCCCEEEEEEECCCCCCCHHHEEEEEECCCHHHHHHHHH
GIPFEGDAFPYPFMLGDVHLAFPGDLKPARGIALRLMRLREDTAPDMFIAIPLPEHGRYR
CCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
VSMLAPEYLAAAGSGVAHGIQSERPGPSLGDIQAVADRIAPGEIVVGDLRWSSQFRISMR
EEEECHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEE
LAARYREGQVFLAGDACHIHPPTGGQGMNTGIQDAYNLAWKLALVMRGKAPASLLDSYEA
EEEECCCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHH
ERRPVAEDVIARTVAESVNIGGSGGARDRLADTQIRVSYAGLAGAALVGGAAAPEGLPGD
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCC
RLPVAGDRAPDALGLRRRGVGAPLRMFQLLRGTDFVVVVAVETPGALVAAEALGARLAES
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
GLPVRVVAVAPAGASLADPYKVSLVADEAGAFAATYGLGAGEAVLVRPDGYLAWRGHAEQ
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCEEEECCCHHH
VVAALRQVAGAG
HHHHHHHHHCCC
>Mature Secondary Structure 
GRQDSGQNSAQAPADVIVVGAGPVGLTLAAELARHKVRCRILEKAVAPSLYCRALGVTP
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
RTLEVYEDMGVVRAMIDAGLWFEGLCLAVDGAPARTIAPDLSDLPYGMIGIPQPVTERIL
CHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCHHHHHHHH
TAHLAGFGIDVERGVAVNGLAQAGEKVRLDLVGPDGREDFAHARFVVGCDGAHSFVRHAL
HHHHHHCCCCCCCCCEECHHHHCCCEEEEEEECCCCCCCHHHEEEEEECCCHHHHHHHHH
GIPFEGDAFPYPFMLGDVHLAFPGDLKPARGIALRLMRLREDTAPDMFIAIPLPEHGRYR
CCCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
VSMLAPEYLAAAGSGVAHGIQSERPGPSLGDIQAVADRIAPGEIVVGDLRWSSQFRISMR
EEEECHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEE
LAARYREGQVFLAGDACHIHPPTGGQGMNTGIQDAYNLAWKLALVMRGKAPASLLDSYEA
EEEECCCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHH
ERRPVAEDVIARTVAESVNIGGSGGARDRLADTQIRVSYAGLAGAALVGGAAAPEGLPGD
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCC
RLPVAGDRAPDALGLRRRGVGAPLRMFQLLRGTDFVVVVAVETPGALVAAEALGARLAES
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
GLPVRVVAVAPAGASLADPYKVSLVADEAGAFAATYGLGAGEAVLVRPDGYLAWRGHAEQ
CCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCEEEECCCHHH
VVAALRQVAGAG
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]